DNA binding sites are a type of binding site found in DNA where other molecules may bind. DNA binding sites are distinct from other binding sites in that (1) they are part of a DNA sequence (e.g. a genome) and (2) they are bound by DNA-binding proteins. DNA binding sites are often associated with specialized proteins known as transcription factors, and are thus linked to transcriptional regulation. The sum of DNA binding sites of a specific transcription factor is referred to as its cistrome. DNA binding sites also encompasses the targets of other proteins, like restriction enzymes, site-specific recombinases (see site-specific recombination) and methyltransferases.[1]
DNA binding sites can be thus defined as short DNA sequences (typically 4 to 30 base pairs long, but up to 200 bp for recombination sites) that are specifically bound by one or more DNA-binding proteins or protein complexes. It has been reported that some binding sites have potential to undergo fast evolutionary change.[2]
^Halford E.S.; Marko J.F. (2004). "How do site-specific DNA-binding proteins find their targets?". Nucleic Acids Research. 32 (10): 3040–3052. doi:10.1093/nar/gkh624. PMC 434431. PMID 15178741.
^Borneman, A.R.; Gianoulis, T.A.; Zhang, Z.D.; Yu, H.; Rozowsky, J.; Seringhaus, M.R.; Wang, L.Y.; Gerstein, M. & Snyder, M. (2007). "Divergence of transcription factor binding sites across related yeast species". Science. 317 (5839): 815–819. Bibcode:2007Sci...317..815B. doi:10.1126/science.1140748. PMID 17690298. S2CID 21535866.
sequence-specific DNA-binding factor) is a protein that controls the rate of transcription of genetic information from DNA to messenger RNA, by binding to a specific...
to the CpG site. It is used in mammals for antiviral defense. Various protein engineering techniques can be used to alter the DNA-binding specificity...
noncovalent binding of DnaG to the DnaB helicase protein. The zinc-binding domain, the domain responsible for recognizing sequence specific DNAbindingsites, is...
mediator complex must be attached to a DNAbindingsite called a promoter region before RNAP can initiate the DNA unwinding at that position. RNAP not only...
is an enzyme that cleaves DNA into fragments at or near specific recognition sites within molecules known as restriction sites. Restriction enzymes are...
conserved DNA repeats that are specifically recognized by DnaA (called DnaA-boxes), an AT-rich DNA unwinding element (DUE), and bindingsites for proteins...
molecular biology, bacterial DNAbinding proteins are a family of small, usually basic proteins of about 90 residues that bind DNA and are known as histone-like...
about half of EGR1 bindingsites are located in promoters and half in enhancers. The binding of EGR1 to its target DNAbindingsite is insensitive to cytosine...
spoken articles) DNA at Curlie DNAbindingsite prediction on protein DNA the Double Helix Game From the official Nobel Prize web siteDNA under electron...
molecular biology, a bindingsite is a region on a macromolecule such as a protein that binds to another molecule with specificity. The binding partner of the...
create sequence logos, related DNA, RNA or protein sequences, or DNA sequences that have common conserved bindingsites, are aligned so that the most conserved...
about half of EGR1 bindingsites are located in promoters and half in enhancers. The binding of EGR1 to its target DNAbindingsite is insensitive to cytosine...
DNA adenine methyltransferase identification, often abbreviated DamID, is a molecular biology protocol used to map the bindingsites of DNA- and chromatin-binding...
many eukaryotic genomes with the difference being their diverged DNAbindingsites. The most widely studied origin recognition complex is that of Saccharomyces...
genes starts with the binding of the enzyme RNA polymerase (RNAP), a DNA-binding protein, which binds to a specific DNAbindingsite, the promoter, immediately...
regions in DNA, which was achieved thanks to the appearance of methods to analyze transcription factor bindingsites, DNA methylation sites, chromatin...
Whitlock JP (April 1992). "Protein-DNA interactions at a dioxin-responsive enhancer. Mutational analysis of the DNA-bindingsite for the liganded Ah receptor"...
more than one DNAbindingsite, and thus can hold a single strand and double strand together. This feature makes it possible to catalyze a DNA synapsis reaction...
interactions with DNA. ChIP-seq combines chromatin immunoprecipitation (ChIP) with massively parallel DNA sequencing to identify the bindingsites of DNA-associated...