DNA adenine methyltransferase identification information
Lab technique
DNA adenine methyltransferase identification, often abbreviated DamID,[1] is a molecular biology protocol used to map the binding sites of DNA- and chromatin-binding proteins in eukaryotes. DamID identifies binding sites by expressing the proposed DNA-binding protein as a fusion protein with DNA methyltransferase. Binding of the protein of interest to DNA localizes the methyltransferase in the region of the binding site. Adenine methylation does not occur naturally in eukaryotes and therefore adenine methylation in any region can be concluded to have been caused by the fusion protein, implying the region is located near a binding site. DamID is an alternate method to ChIP-on-chip or ChIP-seq.[2]
^van Steensel B, Henikoff S (April 2000). "Identification of in vivo DNA targets of chromatin proteins using tethered dam methyltransferase". Nature Biotechnology. 18 (4): 424–8. doi:10.1038/74487. PMID 10748524. S2CID 30350384.
DNAadeninemethyltransferaseidentification, often abbreviated DamID, is a molecular biology protocol used to map the binding sites of DNA- and chromatin-binding...
themselves from infection by bacteriophage.[citation needed] E. coli DNAadeninemethyltransferase (Dam) is an enzyme of ~32 kDa that does not belong to a...
include protein methyltransferases, DNA/RNA methyltransferases, natural product methyltransferases, and non-SAM dependent methyltransferases. SAM is the classical...
up foreign DNA in a process called restriction digestion; meanwhile, host DNA is protected by a modification enzyme (a methyltransferase) that modifies...
after DNA repair was allowed.[citation needed] At least four articles report the recruitment of DNAmethyltransferase 1 (DNMT1) to sites of DNA double-strand...
three DNAmethyltransferases in M. tuberculosis, Mycobacterial AdenineMethyltransferases A (MamA), B (MamB), and C (MamC). All three are adenine methyltransferases...
DNA microarray (also commonly known as DNA chip or biochip) is a collection of microscopic DNA spots attached to a solid surface. Scientists use DNA microarrays...
transcription and DNA repair. Additionally, origin sequences commonly have high AT-content across all kingdoms, since repeats of adenine and thymine are...
unique chromosome regions distributed near oriC (the origin of chromosomal DNA replication), and these regions are collectively referred to as the “oriC...
This occurs via phase variation of a single DNAmethyltransferase. Phase variation of methyltransferase expression results in differential methylation...
Nicotinamide N-methyltransferase (NNMT) is an enzyme that in humans is encoded by the NNMT gene. NNMT catalyzes the methylation of nicotinamide and similar...
methionine by the folate-independent enzyme betaine-homocysteine methyltransferase (BHMT).) MTHFR contains a bound flavin cofactor and uses NAD(P)H as...
conventional DNAmethyltransferase; this would suggest that other mechanisms other than DNA methylation are also involved. Within an organism, DNA methylation...
Histone-lysine N-methyltransferase 2A, also known as acute lymphoblastic leukemia 1 (ALL-1), myeloid/lymphoid or mixed-lineage leukemia 1 (MLL1), or zinc...
covalently attached to the C5 position of a cytosine nucleotide by a DNAmethyltransferase (DNMT) enzyme. These dinucleotide repeats where cytosine is followed...
Buchroithner J, Pichler J, et al. (January 2010). "O6-Methylguanine DNAmethyltransferase protein expression in tumor cells predicts outcome of temozolomide...
m5C "writers" have been identified: NOP2/SUN RNA methyltransferase (NSUN) and DNAmethyltransferase-2. It is important to note that DNMT-2 is a protein...
carrier protein KedY2 and subsequently chlorinated by KedY3, an flavin adenine dinucleotide-dependent halogenase. Insight into the biosynthesis of the...
"A human mitochondrial transcription factor is related to RNA adeninemethyltransferases and binds S-adenosylmethionine". Mol Cell Biol. 22 (4): 1116–25...
the methyl group of methyl-M4MPT is transferred to coenzyme M via a methyltransferase-catalyzed reaction. CH 3 − H 4 MPT + HS − CoM ⟶ CH 3 − S − CoM + H...
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