E. coli K-12 substr. MG1655, Bacillus stearothermophilus
Symbol
dnaG
Alt. symbols
dnaP
Entrez
947570
PDB
1D0Q, 1DD9, 1DDE, 1EQ9, 2R6A, 2R6C
RefSeq (Prot)
NP_417538
UniProt
P0ABS5
Other data
EC number
2.7.7.7
Chromosome
chromosome: 3.21 - 3.21 Mb
Search for
Structures
Swiss-model
Domains
InterPro
DnaG is a bacterial DNA primase and is encoded by the dnaG gene. The enzyme DnaG, and any other DNA primase, synthesizes short strands of RNA known as oligonucleotides during DNA replication. These oligonucleotides are known as primers because they act as a starting point for DNA synthesis. DnaG catalyzes the synthesis of oligonucleotides that are 10 to 60 nucleotides (the fundamental unit of DNA and RNA) long, however most of the oligonucleotides synthesized are 11 nucleotides.[1] These RNA oligonucleotides serve as primers, or starting points, for DNA synthesis by bacterial DNA polymerase III (Pol III). DnaG is important in bacterial DNA replication because DNA polymerase cannot initiate the synthesis of a DNA strand, but can only add nucleotides to a preexisting strand.[2] DnaG synthesizes a single RNA primer at the origin of replication. This primer serves to prime leading strand DNA synthesis. For the other parental strand, the lagging strand, DnaG synthesizes an RNA primer every few kilobases (kb). These primers serve as substrates for the synthesis of Okazaki fragments.[3]
In E. coli DnaG associates through noncovalent interactions with bacterial replicative helicase DnaB to perform its primase activity, with three DnaG primase proteins associating with each DnaB helicase to form the primosome.[4] Primases tend to initiate synthesis at specific three nucleotide sequences on single-stranded DNA (ssDNA) templates and for E. coli DnaG the sequence is 5'-CTG-3'.[1]
DnaG contains three separate protein domains: a zinc binding domain, an RNA polymerase domain, and a DnaB helicase binding domain. There are several bacteria that use the DNA primase DnaG. A few organisms that have DnaG as their DNA primase are Escherichia coli (E. coli), Bacillus stearothermophilus, and Mycobacterium tuberculosis (MTB). E. coli DnaG has a molecular weight of 60 kilodaltons (kDa) and contains 581 amino acids.
^ abCite error: The named reference DNA Primase was invoked but never defined (see the help page).
^Russell P (2009). iGenetics: A Molecular Approach (3rd ed.). Benjamin Cummings. pp. 42–43. ISBN 978-0321772886.
^Nelson D, Cox M (2008). Lehninger Principles of Biochemistry (5th ed.). New York: W.H. Freeman and Company. pp. 986–989. ISBN 978-0716771081.
^Cite error: The named reference Bailey et al was invoked but never defined (see the help page).
DnaG is a bacterial DNA primase and is encoded by the dnaG gene. The enzyme DnaG, and any other DNA primase, synthesizes short strands of RNA known as...
which interacts with DnaB-ID. The Toprim fold is also found in topoisomerase and mitochrondrial Twinkle primase/helicase. Some DnaG-like (bacteria-like;...
according to base pairing rules (A with T and C with G), with hydrogen bonds to make double-stranded DNA. The complementary nitrogenous bases are divided...
single stranded DNA during DNA replication. The primosome consists of seven proteins: DnaG primase, DnaB helicase, DnaC helicase assistant, DnaT, PriA, Pri...
other dnaB-dnaC complex will complex with dnaA from the dnaC. After the assembly of dnaG onto the N-terminus of dnaB, dnaC is released and dnaB will be...
bacteria and archaea/eukaryotes. Bacteria use a primase belonging to the DnaG protein superfamily which contains a catalytic domain of the TOPRIM fold...
with DnaG (a primase) to form the only primer for the leading strand and to add RNA primers on the lagging strand. The interaction between DnaG and DnaB is...
DNA methylation is a biological process by which methyl groups are added to the DNA molecule. Methylation can change the activity of a DNA segment without...
cycle. Initially when DnaB binds to dnaA, it is associated with dnaC, a negative regulator. After DnaC dissociates, DnaB binds dnaG. The N-terminal has...
DNA profiling (also called DNA fingerprinting and genetic fingerprinting) is the process of determining an individual's deoxyribonucleic acid (DNA) characteristics...
"Toprim--a conserved catalytic domain in type IA and II topoisomerases, DnaG-type primases, OLD family nucleases and RecR proteins". Nucleic Acids Research...
interacts with the DnaA bound to the single-stranded DNA to recruit the DnaB helicase, which will continue to unwind the DNA as the DnaG primase lays down...
Mitochondrial DNA (mtDNA or mDNA) is the DNA located in mitochondria, cellular organelles within eukaryotic cells that convert chemical energy from food...
a T-segment of DNA (T- from transferring) in a cavity between GyrB subunits. On a next step the enzyme cleaves a G-segment of DNA (G- from gate) making...
II topoisomerases, type IA topoisomerases, and bacterial primase (DnaG), a central DNA-binding core (which structurally forms a heart-shaped structure)...
material between generations requires timely and accurate duplication of DNA by semiconservative replication prior to cell division to ensure each daughter...
DNA contains the nucleobase thymine instead of uracil. DUT, the human version of this gene dnaA dnaB dnaC dnaE dnaGdnaH dnaI dnaN dnaP dnaQ dnaX dnaZ...
DNA repair is a collection of processes by which a cell identifies and corrects damage to the DNA molecules that encode its genome. In human cells, both...
of DNA in their genome, called double-stranded DNA (dsDNA) viruses, and those that have one strand of DNA in their genome, called single-stranded DNA (ssDNA)...
of DNA. These enzymes are essential for DNA replication and usually work in groups to create two identical DNA duplexes from a single original DNA duplex...
The CpG sites or CG sites are regions of DNA where a cytosine nucleotide is followed by a guanine nucleotide in the linear sequence of bases along its...
(a CpG site). In mammals, DNA methyltransferases (which add methyl groups to DNA bases) exhibit a strong sequence preference for cytosines at CpG sites...