Crystal structure of a tetramer of thymidine kinase from U. urealyticum (where the monomers are color cyan, green, red, and magenta respectively) in complex with thymidine (space-filling model, carbon = white, oxygen = red, nitrogen = blue).[1]
Identifiers
EC no.
2.7.1.21
CAS no.
9002-06-6
Databases
IntEnz
IntEnz view
BRENDA
BRENDA entry
ExPASy
NiceZyme view
KEGG
KEGG entry
MetaCyc
metabolic pathway
PRIAM
profile
PDB structures
RCSB PDB PDBe PDBsum
Gene Ontology
AmiGO / QuickGO
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articles
PubMed
articles
NCBI
proteins
Thymidine kinase
Identifiers
Symbol
TK
Pfam
PF00265
Pfam clan
CL0023
InterPro
IPR001267
PROSITE
PDOC00524
Available protein structures:
Pfam
structures / ECOD
PDB
RCSB PDB; PDBe; PDBj
PDBsum
structure summary
PDB
1W4R, 1XBT, 1XX6, 2B8T
Thymidine kinase 1, soluble
Identifiers
Symbol
TK1
NCBI gene
7083
HGNC
11830
OMIM
188300
RefSeq
NM_003258
UniProt
P04183
Other data
EC number
2.7.1.21
Locus
Chr. 17 q23.2-25.3
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Structures
Swiss-model
Domains
InterPro
Thymidine kinase 2, mitochondrial
Identifiers
Symbol
TK2
NCBI gene
7084
HGNC
11831
OMIM
188250
RefSeq
NM_004614
UniProt
O00142
Other data
EC number
2.7.1.21
Locus
Chr. 16 [1]
Search for
Structures
Swiss-model
Domains
InterPro
Thymidine kinase is an enzyme, a phosphotransferase (a kinase): 2'-deoxythymidine kinase, ATP-thymidine 5'-phosphotransferase, EC 2.7.1.21.[2][3] It can be found in most living cells. It is present in two forms in mammalian cells, TK1 and TK2. Certain viruses also have genetic information for expression of viral thymidine kinases.
Thymidine kinase catalyzes the reaction:
Thd + ATP → TMP + ADP
where Thd is (deoxy)thymidine, ATP is adenosine triphosphate, TMP is (deoxy)thymidine monophosphate and ADP is adenosine diphosphate.
Thymidine kinases have a key function in the synthesis of DNA and therefore in cell division, as they are part of the unique reaction chain to introduce thymidine into the DNA. Thymidine is present in the body fluids as a result of degradation of DNA from food and from dead cells. Thymidine kinase is required for the action of many antiviral drugs. It is used to select hybridoma cell lines in production of monoclonal antibodies. In clinical chemistry it is used as a proliferation marker in the diagnosis, control of treatment and follow-up of malignant disease, mainly of hematological malignancies.
^Cite error: The named reference PDB_2B8T was invoked but never defined (see the help page).
^Cite error: The named reference Kit_1985 was invoked but never defined (see the help page).
^Cite error: The named reference Wintersberger_1997 was invoked but never defined (see the help page).
Thymidinekinase is an enzyme, a phosphotransferase (a kinase): 2'-deoxythymidine kinase, ATP-thymidine 5'-phosphotransferase, EC 2.7.1.21. It can be...
Thymidinekinase is an enzyme, a phosphotransferase (a kinase): 2'-deoxythymidine kinase, ATP-thymidine 5'-phosphotransferase, EC 2.7.1.21 that catalyzes...
Phosphotransferase Signal transduction ThymidinekinaseThymidinekinase in clinical chemistry Thymidylate kinase Wall-associated kinase Siebold C, Arnold I, Garcia-Alles...
Thymidinekinase 1, soluble (gene name TK1), is a human thymidinekinase. Two forms of this protein have been identified in animal cells, one in cytosol...
Thymidinekinase from herpesvirus is a sub-family of thymidinekinases that catalyses the transfer of phospho group of ATP to thymidine to generate thymidine...
monophosphate form by viral thymidinekinase, which is more effective (3000 times) in phosphorylation of aciclovir than cellular thymidinekinase. Subsequently, the...
of resistance in HSV include deficient viral thymidinekinase; and mutations to viral thymidinekinase or DNA polymerase, altering substrate sensitivity...
Thymidylate kinase (EC 2.7.4.9; dTMP kinase) catalyzes the phosphorylation of thymidine 5'-monophosphate (dTMP) to form thymidine 5'-diphosphate (dTDP)...
alternative source is the thymidine present in the HAT medium that can be absorbed by the cells and phosphorylated by thymidinekinase (TK) into TMP. The synthesis...
deoxynucleoside thymidine. Thymidinekinase can then phosphorylate the 5’-carbon of this compound into thymidine monophosphate (TMP). Thymidylate kinase can phosphorylate...
first functional promoters in eukaryotes was discovered in HSV (of the thymidinekinase gene) and the virion protein VP16 is one of the most-studied transcriptional...
Hallek, M.; Wanders, L.; Strohmeyer, S.; Emmerich, B. (July 1992). "Thymidinekinase: a tumor marker with prognostic value for non-Hodgkin's lymphoma and...
inactive in its initial form. Within a virally infected cell a viral thymidinekinase adds a phosphate group to the penciclovir molecule; this is the rate-limiting...
Bacchetti S, Graham FL (April 1977). "Transfer of the gene for thymidinekinase to thymidinekinase-deficient human cells by purified herpes simplex viral DNA"...
virus, encoding a thymidinekinase suicide gene, has progressed to phase III clinical trials. The herpes simplex virus thymidinekinase phosphorylates the...
play a role in the 'bystander effect' of the herpes simplex virus thymidinekinase/ganciclovir system in vitro". Gene Ther. 3 (1): 85–92. PMID 8929915...
mediated by mutation(s) of the viral UL23 gene, which encodes the viral thymidinekinase (TK), then the pyrophosphate analogue foscarnet may be effective as...
inhibit growth of the host organism upon selection. An example would be thymidinekinase, which makes the host sensitive to ganciclovir selection.[citation...
expanded, and compounds were tested in both TK+ (thymidinekinase competent) and TK- (thymidinekinase deficient) cell lines. None of the phosphoramidates...
proteins involved in DNA replication (for example DNA polymerase, thymidinekinase, dihydrofolate reductase and cdc6), and chromosomal replication (replication...
toolkit Telecine, a process of transferring motion picture film to video Thymidinekinase, a protein Transketolase, an enzyme in the pentose phosphate pathway...
Lavie A (February 2009). "Extending thymidinekinase activity to the catalytic repertoire of human deoxycytidine kinase". Biochemistry. 48 (6): 1256–63....
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