Selectable markers are a genes introduced into a cell, especially a bacterium or to cells in culture, that confer a traits suitable for artificial selection. They are a type of reporter gene used in laboratory microbiology, molecular biology, and genetic engineering to indicate the success of a transfection or other procedure meant to introduce foreign DNA into a cell. Selectable markers are often antibiotic resistance genes: bacteria that have been subjected to a procedure to introduce foreign DNA are grown on a medium containing an antibiotic, and those bacterial colonies that can grow have successfully taken up and expressed the introduced genetic material. Normally, the genes encoding resistance to antibiotics such as ampicillin, chloramphenicol, tetracycline, kanamycin, etc., are considered useful selectable markers for E. coli.
Selectablemarkers are a genes introduced into a cell, especially a bacterium or to cells in culture, that confer a traits suitable for artificial selection...
two sub-types of these marker genes: a selectablemarker and a marker for screening. In metagenomics and phylogenetics, a marker gene is an orthologous...
transgene as well as a plant selectablemarker. Outside of the T-DNA, the binary vector also contains a bacterial selectablemarker and an origin of replication...
encoding a selectablemarker is a common element within DNA constructs and is used to select for properly transformed cells. The selectablemarker chosen...
in two different organisms may also require two selectablemarkers, although some selectablemarkers such as resistance to zeocin and hygromycin B are...
expressing them are easily identified and measured, or because they are selectablemarkers. Reporter genes are often used as an indication of whether a certain...
of a yeast shuttle vector includes an origin of replication and a selectablemarker, e.g. antibiotic resistance, beta lactamase, beta galactosidase. The...
eukaryotic cell lines when used in conjunction with vectors containing a selectablemarker for Zeocin resistance. Zeocin is considerably cheaper than phleomycin...
contain an origin of replication, a multiple cloning site, and a selectablemarker. Certain vectors can carry additional regulatory elements based on...
cerevisiae. Additionally, selectablemarker genes, such as antibiotic resistance and a visible marker, are utilized to select transformed yeast cells....
Gene cassette, certain vectors that are normally used to confer a selectablemarker on an organism Cassette cogset, a set of multiple sprockets on a bicycle...
trapping cassette consisting of a promoterless reporter gene and/or selectable genetic marker, flanked by an upstream 3' splice site (splice acceptor; SA) and...
often accompanied by promoter and terminator regions as well as a selectablemarker gene. The added gene may itself be modified to make it express more...
a gene (i.e. a knockout), indicated by the presence of a positive selectablemarker) is crossed with a mouse of a constant genetic background, the average...
regeneration is not necessary. Selectablemarkers are used to easily differentiate transformed from untransformed cells. These markers are usually present in...
elements, including a promoter and terminator region and often a selectablemarker. A number of techniques are available for inserting the isolated gene...
A genetic marker is a gene or DNA sequence with a known location on a chromosome that can be used to identify individuals or species. It can be described...
features of a suitable gene cassette including but not limited to (1) selectablemarker (2) flanking sequences (3) gene of interest (4) promoter sequences...
spectinomycin and ampicillin antibiotics. Kanamycin resistance is used as a selectablemarker. A. brasilense has a high evolutionary adaptation rate driven by codon...
genetic markers in yeast genetic modification. While URA3 is a powerful selectablemarker, it has a high background. This background is because cells that pick...
containing the selectablemarker gene encoded by the vector are able to survive. When bacterial cells are used as host organisms, the selectablemarker is usually...
of colonies present on the plates. These vectors generally have a selectablemarker allowing the differentiation of clones containing an insert from those...
measures of the capacity an antibiotic to select for transfected (resistant) cells that contain a selectablemarker, while killing untransfected (sensitive)...
Alba. Other features on pGLO, like most other plasmids, include a selectablemarker and an MCS (multiple cloning site) located at the end of the GFP gene...
being able to induce single hit mutations, being able to incorporate selectablemarkers in strain construction, and being able to recover genes after mutagenesis...
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