Thymidine kinase from herpesvirus is a sub-family of thymidine kinases that catalyses the transfer of phospho group of ATP to thymidine to generate thymidine monophosphate, which serves as a substrate during viral DNA replication.[2][3]
Its presence in herpesvirus-infected cells is used to activate a range of antivirals against herpes infection, and thus specifically target the therapy towards infected cells only.
Such antivirals include:
Purine analogues of guanine: Aciclovir, Famciclovir, Ganciclovir, Penciclovir, Valaciclovir, Valganciclovir
Vidarabine
Pyrimidine analogues of uridine: Idoxuridine, Trifluridine
Brivudine
Mutations in the gene coding thymidine kinase in herpes viruses can endow the virus with resistance to aciclovir. In these situations, alternative medications that are of use include other guanine analogues such as famciclovir, valaciclovir and penciclovir.[4][5]
^Champness JN, Bennett MS, Wien F, Visse R, Summers WC, Herdewijn P, et al. (August 1998). "Exploring the active site of herpes simplex virus type-1 thymidine kinase by X-ray crystallography of complexes with aciclovir and other ligands". Proteins. 32 (3): 350–361. doi:10.1002/(SICI)1097-0134(19980815)32:3<350::AID-PROT10>3.0.CO;2-8. PMID 9715911. S2CID 20167407.
^Vogt J, Perozzo R, Pautsch A, Prota A, Schelling P, Pilger B, et al. (December 2000). "Nucleoside binding site of herpes simplex type 1 thymidine kinase analyzed by X-ray crystallography". Proteins. 41 (4): 545–553. doi:10.1002/1097-0134(20001201)41:4<545::AID-PROT110>3.0.CO;2-8. PMID 11056041. S2CID 2829079.
^Brown DG, Visse R, Sandhu G, Davies A, Rizkallah PJ, Melitz C, et al. (October 1995). "Crystal structures of the thymidine kinase from herpes simplex virus type-1 in complex with deoxythymidine and ganciclovir". Nature Structural Biology. 2 (10): 876–881. doi:10.1038/nsb1095-876. PMID 7552712. S2CID 3223579.
^Frobert E, Ooka T, Cortay JC, Lina B, Thouvenot D, Morfin F (March 2005). "Herpes simplex virus thymidine kinase mutations associated with resistance to acyclovir: a site-directed mutagenesis study". Antimicrobial Agents and Chemotherapy. 49 (3): 1055–1059. doi:10.1128/AAC.49.3.1055-1059.2005. PMC 549244. PMID 15728902.
^Suzuki M, Okuda T, Shiraki K (November 2006). "Synergistic antiviral activity of acyclovir and vidarabine against herpes simplex virus types 1 and 2 and varicella-zoster virus". Antiviral Research. 72 (2): 157–161. doi:10.1016/j.antiviral.2006.05.001. PMID 16797734.
and 18 Related for: Thymidine kinase from herpesvirus information
Thymidinekinasefromherpesvirus is a sub-family of thymidinekinases that catalyses the transfer of phospho group of ATP to thymidine to generate thymidine...
Thymidinekinase is an enzyme, a phosphotransferase (a kinase): 2'-deoxythymidine kinase, ATP-thymidine 5'-phosphotransferase, EC 2.7.1.21. It can be...
Thymidinekinase is an enzyme, a phosphotransferase (a kinase): 2'-deoxythymidine kinase, ATP-thymidine 5'-phosphotransferase, EC 2.7.1.21 that catalyzes...
monophosphate form by viral thymidinekinase, which is more effective (3000 times) in phosphorylation of aciclovir than cellular thymidinekinase. Subsequently, the...
first functional promoters in eukaryotes was discovered in HSV (of the thymidinekinase gene) and the virion protein VP16 is one of the most-studied transcriptional...
Phosphotransferase Signal transduction ThymidinekinaseThymidinekinase in clinical chemistry Thymidylate kinase Wall-associated kinase Siebold C, Arnold I, Garcia-Alles...
alphaherpesvirus 1; McHV-1; formerly Macacine herpesvirus 1, Cercopithecine herpesvirus 1, CHV-1), Herpesvirus simiae, or Herpes virus B is the Simplexvirus...
studies of basic processes during lytic herpesvirus infection, and for unravelling molecular mechanisms of herpesvirus neurotropism. In 1902, a Hungarian veterinarian...
Marschall M. (2006). "Endotheliotropic elephant herpesvirus, the first betaherpesvirus with a thymidinekinase gene". Journal of General Virology. 87 (Pt 10):...
of resistance in HSV include deficient viral thymidinekinase; and mutations to viral thymidinekinase or DNA polymerase, altering substrate sensitivity...
inactive in its initial form. Within a virally infected cell a viral thymidinekinase adds a phosphate group to the penciclovir molecule; this is the rate-limiting...
degrading without being excreted in urine. Sorivudine is phosphorylated by thymidinekinase activity in the body and is absorbed into the virus's DNA instead of...
the expression of several lytic gene products, including ICP4 and ThymidineKinase. LAT expression results in changes to Histones, thus converting portions...
which specifically target herpes virus-infected cells using viral thymidinekinase (TK). Viral TK phosphorylates aciclovir into its monophosphate form...
case the study uses the transfer of the Herpes simplex virus type I thymidinekinase (HSVtk) as the cytotoxic gene. Hela cells were used in these studies...
The HSV virus used as an oncolytic virus has retained their native thymidinekinase gene which allows it to be targeted with antiretroviral therapy in...
Global Information