Separating components of a cell while preserving their functions
In cell biology, cell fractionation is the process used to separate cellular components while preserving individual functions of each component.[1] This is a method that was originally used to demonstrate the cellular location of various biochemical processes. Other uses of subcellular fractionation is to provide an enriched source of a protein for further purification, and facilitate the diagnosis of various disease states.[2]
^Alberts, B; Johnson, A (2002). "Fractionation of Cells". Molecular Biology of the Cell. 4th edition.
^Ninfa, Alexander J. (2010). Fundamental Laboratory Approaches for Biochemistry and Biotechnology. United States of America: John Wiley & Sons, INC. p. 209. ISBN 978-0-470-08766-4.
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In cell biology, cellfractionation is the process used to separate cellular components while preserving individual functions of each component. This is...
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may be seen as structures inside the cell. Different techniques including freeze-fracturing and cellfractionation may be used to overcome the problems...
cellular extracts. Therefore, they tried a more arduous procedure of cellfractionation, by which cellular components are separated based on their sizes using...
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red blood cells from the remaining components. In erythrocytapheresis, centrifugation is the most commonly used red blood cellfractionation method. This...
designate the antibody. Cell populations are usually defined using a '+' or a '−' symbol to indicate whether a certain cellfraction expresses or lacks a...
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discovered that not all cells in a population proliferate at similar rates. A population's "growth fraction" – or the fraction of the population that was...