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Cell fractionation information


In cell biology, cell fractionation is the process used to separate cellular components while preserving individual functions of each component.[1] This is a method that was originally used to demonstrate the cellular location of various biochemical processes. Other uses of subcellular fractionation is to provide an enriched source of a protein for further purification, and facilitate the diagnosis of various disease states.[2]

  1. ^ Alberts, B; Johnson, A (2002). "Fractionation of Cells". Molecular Biology of the Cell. 4th edition.
  2. ^ Ninfa, Alexander J. (2010). Fundamental Laboratory Approaches for Biochemistry and Biotechnology. United States of America: John Wiley & Sons, INC. p. 209. ISBN 978-0-470-08766-4.

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Cell fractionation

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In cell biology, cell fractionation is the process used to separate cellular components while preserving individual functions of each component. This is...

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Fractionation

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proteins fraction. Copurification List of purification methods in chemistry Transposition cipher#Fractionation Gold, Victor, ed. (2019). 'fractionation' - The...

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Cell biology

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culture, and cell fractionation. These have allowed for and are currently being used for discoveries and research pertaining to how cells function, ultimately...

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Mitochondrion

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technical snag in cell fractionation techniques, the alleged ER vesicle contaminants that invariably appeared in the mitochondrial fraction have been re-identified...

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Organelle

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microscopy, and can also be purified by cell fractionation. There are many types of organelles, particularly in eukaryotic cells. They include structures that make...

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Dose fractionation

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effects on healthy cells. This maximizes the effect of radiation on cancer and minimizes the negative side effects. A typical fractionation scheme divides...

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Homogenizer

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including Cell Lysor, Disperser, High Shear Mixer, Homogenizer, Polytron, Rotor Stator Homogenizer, Sonicator or Tissue Tearor. Cell fractionation is done...

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George Emil Palade

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innovations in electron microscopy and cell fractionation which together laid the foundations of modern molecular cell biology, the most notable discovery...

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Albert Claude

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in cell biology. In 1930 he developed the technique of cell fractionation, by which he discovered the agent of the Rous sarcoma, components of cell organelles...

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Centrifugation

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purification of mammalian cells, fractionation of subcellular organelles, fractionation of membrane vesicles, fractionation of macromolecules and macromolecular...

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Christian de Duve

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An unrelated enzyme (of the cell fractionation procedure) had come from membranous fractions that were known to be cell organelles. In 1955, de Duve...

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Field flow fractionation

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Field-flow fractionation, abbreviated FFF, is a separation technique invented by J. Calvin Giddings. The technique is based on separation of colloidal...

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Blood fractionation

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separated into its own fractions, see Blood plasma fractionation), the buffy coat, which is a thin layer of leukocytes (white blood cells) mixed with platelets...

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Hematocrit

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for the hematocrit, such as packed cell volume (PCV), volume of packed red cells (VPRC), or erythrocyte volume fraction (EVF). The term hematocrit (or haematocrit...

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Isotope fractionation

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Isotope fractionation describes fractionation processes that affect the relative abundance of isotopes, phenomena which are taken advantage of in isotope...

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Fractionation of carbon isotopes in oxygenic photosynthesis

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Isotope fractionation due to Rubisco (form I) carboxylation alone is predicted to be a 28‰ depletion, on average. However, fractionation values vary...

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Visual artifact

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may be seen as structures inside the cell. Different techniques including freeze-fracturing and cell fractionation may be used to overcome the problems...

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Lysosome

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cellular extracts. Therefore, they tried a more arduous procedure of cell fractionation, by which cellular components are separated based on their sizes using...

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Eukaryote

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-⁠əts) constitute the domain of Eukarya or Eukaryota, organisms whose cells have a membrane-bound nucleus. All animals, plants, fungi, and many unicellular...

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Assay

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hybridization, combined to PCR, Computational genomics, and Transfection; Cell fractionation or Immunoprecipitation Migration assays, Chemotaxis assay Secretion...

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Cell survival curve

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the total survival fraction being the product of the two functions, the model is aptly called linear-quadratic. Dose fractionation Dose–response relationship...

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Secondary cell wall

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The secondary cell wall is a structure found in many plant cells, located between the primary cell wall and the plasma membrane. The cell starts producing...

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Erythrocytapheresis

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red blood cells from the remaining components. In erythrocytapheresis, centrifugation is the most commonly used red blood cell fractionation method. This...

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Cluster of differentiation

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designate the antibody. Cell populations are usually defined using a '+' or a '−' symbol to indicate whether a certain cell fraction expresses or lacks a...

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Peripheral blood mononuclear cell

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mononuclear cell (PBMC) is any peripheral blood cell having a round nucleus. These cells consist of lymphocytes (T cells, B cells, NK cells) and monocytes...

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Nature Protocols

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PET) Immunological techniques Isolation and Purification (includes cell fractionation and others) Microbiology and virology Molecular biology (includes...

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Outline of cell biology

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releasing molecules from cells. Cell fractionation – Separation of homogeneous sets from a larger population of cells. Cell incubator – The device used...

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G0 phase

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discovered that not all cells in a population proliferate at similar rates. A population's "growth fraction" – or the fraction of the population that was...

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