"PBMC" redirects here. For the internet slang that expands to "problems between monitor and chair", see User error. For the 2009 film, see Paul Blart: Mall Cop.
A peripheral blood mononuclear cell (PBMC) is any peripheral blood cell having a round nucleus.[1] These cells consist of lymphocytes (T cells, B cells, NK cells) and monocytes, whereas erythrocytes and platelets have no nuclei, and granulocytes (neutrophils, basophils, and eosinophils) have multi-lobed nuclei. In humans, lymphocytes make up the majority of the PBMC population, followed by monocytes, and only a small percentage of dendritic cells.[2]
These cells can be extracted from whole blood using ficoll, a hydrophilic polysaccharide that separates layers of blood, and gradient centrifugation,[3] which will separate the blood into a top layer of plasma, followed by a layer of PBMCs (buffy coat) and a bottom fraction of polymorphonuclear cells (such as neutrophils and eosinophils) and erythrocytes. The polymorphonuclear cells can be further isolated by lysing the red blood cells. Basophils are sometimes found in both the denser and the PBMC fractions.[3]
^Delves, Peter, et al. Roitt's Essential Immunology, 11th Ed. ISBN 978-1-4051-3603-7
^The impact of food bioactives on gut health : in vitro and ex vivo models. Verhoeckx, Kitty, 1970-, Cotter, Paul (Paul D.),, European Cooperation in the Field of Scientific and Technical Research (Organization). Cham. 2015-05-19. ISBN 9783319157917. OCLC 908392100.{{cite book}}: CS1 maint: location missing publisher (link) CS1 maint: others (link)
^ abMiyahira, Andrea (22 Nov 2012). "Types of immune cells present in human PBMC". sanguinebio.com. Archived from the original on 22 July 2016. Retrieved 23 Sep 2014.
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diameter of 15–22 μm, monocytes are the largest cell type in peripheralblood. Monocytes are mononuclearcells and the ellipsoidal nucleus is often lobulated/indented...
White bloodcells (scientific name leukocytes), also called immune cells or immunocytes, are cells of the immune system that are involved in protecting...
formed from stem cells in the bone marrow. Monocyte-derived dendritic cells can be generated in vitro from peripheralbloodmononuclearcell (PBMCs). Plating...
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latency. Incubation of peripheralbloodmononuclearcells with novel potent TLR-9 ligand MGN1703 have resulted in enhancement of NK cell effector functions...
Microchimerism in PeripheralBloodMononuclearCell Subsets in Healthy Women and Women With Scleroderma". Blood. 93 (6): 2033–2037. doi:10.1182/blood.V93.6.2033...
information about the cells in a person's blood. The CBC indicates the counts of white bloodcells, red bloodcells and platelets, the concentration of hemoglobin...
nongranulocytes or mononuclear leukocytes) are one of the two types of leukocytes (white bloodcells), the other type being granulocytes. Agranular cells are noted...
Red bloodcells (RBCs), scientific name erythrocytes (from ancient Greek erythros 'red' and kytos 'hollow vessel', with -cyte translated as 'cell' in...
suggest that this virus may latently infect the human sera and peripheralbloodmononuclearcells. Most MCV viruses found in MCC tumors, however, have at least...
interleukin 12 (IL-12) secretion by peripheralbloodmononuclearcells (PBMC) in response to Leishmania antigens. T cells isolated from both cured and asymptomatic...
type of white bloodcell) that kills cancer cells, cells that are infected by intracellular pathogens (such as viruses or bacteria), or cells that are damaged...
Kurloff cells (also known as Foà-Kurloff cells,) were described as mononuclearcells in the peripheralblood and organs of the guinea pig, capybara, paca...
apheresis. Peripheralbloodmononuclearcells (PBMCs) are then separated and collected. The products of leukocyte apheresis are then transferred to a cell-processing...
have developed critically poor blood flow to the legs, the benefit of autotransplantation of autologous mononuclearcells is unclear. Only one randomized...
of cytokine-induced myeloid-derived suppressor cells from normal human peripheralbloodmononuclearcells". Journal of Immunology. 185 (4): 2273–84. doi:10...
descriptions of the presence of papillomavirus DNA in the blood and in the peripheralbloodmononuclearcells. Papillomaviruses were first identified in the early...
formed by plating peripheralbloodmononuclearcells on fibronectin-coated dishes, allowing adhesion and depleting non-adherent cells, and isolating discrete...
evaluated in detecting human disease, such as breast cancer. Peripheralbloodmononuclearcell (PBMC) miRNA expression has been studied as potential biomarker...
By comparison, the mononuclearcells in muscles are much smaller. Some of the mononuclearcells in muscles are endothelial cells (which are about 50–70...
system. They are derived from the earliest wave of mononuclearcells that originate in yolk sac blood islands early in development, and colonize the brain...
"Association between polycyclic aromatic hydrocarbon exposure and peripheralbloodmononuclearcell DNA damage in human volunteers during fire extinction exercises"...
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