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Chemical compound
Propidium iodide (or PI) is a fluorescent intercalating agent that can be used to stain cells and nucleic acids. PI binds to DNA by intercalating between the bases with little or no sequence preference. When in an aqueous solution, PI has a fluorescent excitation maximum of 493 nm (blue-green), and an emission maximum of 636 nm (red). After binding DNA, the quantum yield of PI is enhanced 20-30 fold, and the excitation/emission maximum of PI is shifted to 535 nm (green) / 617 nm (orange-red).[1] Propidium iodide is used as a DNA stain in flow cytometry to evaluate cell viability or DNA content in cell cycle analysis, [2] or in microscopy to visualize the nucleus and other DNA-containing organelles. Propidium Iodide is not membrane-permeable, making it useful to differentiate necrotic, apoptotic and healthy cells based on membrane integrity.[3][4] PI also binds to RNA, necessitating treatment with nucleases to distinguish between RNA and DNA staining.[5] PI is widely used in fluorescence staining and visualization of the plant cell wall.[6]
Propidiumiodide (or PI) is a fluorescent intercalating agent that can be used to stain cells and nucleic acids. PI binds to DNA by intercalating between...
the already dead cells are called vital stains (e.g. trypan blue or propidiumiodide for eukaryotic cells). Those that enter and stain living cells are...
compromise cell membrane integrity. Vital dyes, such as trypan blue or propidiumiodide are normally excluded from the inside of healthy cells; however, if...
stained positively. (Vital stains include dyes like trypan blue and propidiumiodide, which are either too bulky or too charged to cross the cell membrane...
treated with a fluorescent dye that stains DNA quantitatively, such as propidiumiodide (PI) or 4,6-diamidino-2-phenylindole (DAPI). The fluorescence intensity...
membranes are no longer intact. Examples of this type of assay include propidiumiodide, trypan blue, and 7-Aminoactinomycin D (7-AAD). Mitochondrial activity...
fluorescences compared). With this technique, a probe for viability such as propidiumiodide (PI) could also be included in order to exclude dead cells from the...
of the head adheres to the surface of the head prior to deployment. Propidiumiodide staining revealed that only the basal surface of the hook is of nuclear...
through intercalation. Examples of such dyes are ethidium bromide and propidiumiodide. It is an isomer of acridine. Phenanthridine was discovered by Amé...
and GelGreen, marketed as safer and more intense DNA stains Propidiumiodide and propidium monoazide, related dyes "GESTIS-Stoffdatenbank". gestis.dguv...
distinct subsets (e.g. viable, dead, dying etc.). Eosin dye exclusion Propidiumiodide, DNA stain that can differentiate necrotic, apoptotic and normal cells...
probe, and counterstained with 4',6-Diamidino-2-phenylindole (DAPI) or propidiumiodide. Analysis of FISH specimens is done by fluorescence microscopy by a...
detection of zinc increase and the well accepted cell death indicator propidiumiodide also occurred in kidney cells. Using the principles of fluorescent...
index, a measure of leanness of a person (similar to body mass index) Propidiumiodide, a chemical used as a DNA stain Primary immunodeficiency Protease inhibitor...
Zeta toxin induces reversible protective dormancy and permeation to propidiumiodide (PI). This protein family entry consists of several bacterial zeta...
Acridine orange can be used in conjunction with ethidium bromide or propidiumiodide to differentiate between viable, apoptotic, and necrotic cells. Additionally...
fish genomes resulted in significantly different genome sizes when propidiumiodide (PI) and DAPI were used, respectively. For instance, the genome of...
membrane events with the staining of DNA in the cell nucleus with propidiumiodide (PI) or 7-Aminoactinomycin D (AAD-7), distinguishing viable cells from...
1991). "A rapid and simple method for measuring thymocyte apoptosis by propidiumiodide staining and flow cytometry". Journal of Immunological Methods. 139...
cleave the fluorescein first. It is also used in combination with propidiumiodide (PI) to determine viability in eukaryotic cells. Living cells will...
well as surface-expression of proteins. For example, in dead cells, propidiumiodide (PI) can penetrate the nucleus where it binds to DNA. The fluorescent...
nuclear staining with propidiumiodide the maintrac method can distinguish between dead and living cells. Only vital, propidium excluding EpCAM positive...
as N-methyl-D-glucamine (NMDG+) and nucleotide binding dyes such as propidiumiodide (YO-PRO-1). Whether this change in permeability is due to a widening...
stage the membrane becomes abnormally permeable to trypan blue and propidiumiodide, indicating membrane compromise. The final stage is cell death and...
the more of a particular protein is present. Staining with DNA dyes propidiumiodide or 4',6'-diamidino-2-phenylindole (DAPI) allows delineation or sorting...
D384-cells and lung carcinoma A549-cells, using the MTT assay and calcein/propidiumiodide (PI) staining. Both the as-received and the modified nanotubes were...
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