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Chemical compound
Ethidium bromide (or homidium bromide,[2] chloride salt homidium chloride)[3][4] is an intercalating agent commonly used as a fluorescent tag (nucleic acid stain) in molecular biology laboratories for techniques such as agarose gel electrophoresis. It is commonly abbreviated as EtBr, which is also an abbreviation for bromoethane. To avoid confusion, some laboratories have used the abbreviation EthBr for this salt. When exposed to ultraviolet light, it will fluoresce with an orange colour, intensifying almost 20-fold after binding to DNA. Under the name homidium, it has been commonly used since the 1950s in veterinary medicine to treat trypanosomiasis in cattle.[5] The high incidence of antimicrobial resistance makes this treatment impractical in some areas, where the related isometamidium chloride is used instead. Despite its reputation as a mutagen, tests have shown it to have low mutagenicity without metabolic activation.[6][7]
^"GESTIS-Stoffdatenbank". gestis.dguv.de (in German). Retrieved 22 November 2021.
^Cite error: The named reference Hom-Br-PubChem was invoked but never defined (see the help page).
^Cite error: The named reference Kinabo-1993 was invoked but never defined (see the help page).
^Cite error: The named reference Hom-Cl-PubChem was invoked but never defined (see the help page).
^Stevenson P, Sones KR, Gicheru MM, Mwangi EK (May 1995). "Comparison of isometamidium chloride and homidium bromide as prophylactic drugs for trypanosomiasis in cattle at Nguruman, Kenya". Acta Tropica. 59 (2): 77–84. doi:10.1016/0001-706X(94)00080-K. PMID 7676909.
^Lowe, Derek (2016-04-18). "The Myth of Ethidium Bromide". In the Pipeline. Retrieved 2019-02-28.
^"Ethidium Bromide: Swap or Not | UCSB Sustainability". sustainability.ucsb.edu. Retrieved 2023-02-08.
Ethidiumbromide (or homidium bromide, chloride salt homidium chloride) is an intercalating agent commonly used as a fluorescent tag (nucleic acid stain)...
Circular DNA are more strongly affected by ethidiumbromide concentration than linear DNA if ethidiumbromide is present in the gel during electrophoresis...
of the buffer, and the concentration of intercalating dye such as ethidiumbromide if used during electrophoresis. Smaller molecules travel faster than...
and its optical properties are essentially identical to those of ethidiumbromide. When exposed to ultraviolet light, it fluoresces with an orange color...
of one or the other is more a matter of preference and tradition. Ethidiumbromide intercalates and stains DNA, providing a fluorescent red-orange stain...
for ethidiumbromide, a potential human mutagen, as both safer to work with and free from the complex waste disposal issues of ethidiumbromide. However...
bromide, is a chemical compound of the haloalkanes group. It is abbreviated by chemists as EtBr (which is also used as an abbreviation for ethidium bromide)...
upregulation after CNS injuries, promoting axonal regeneration. Injection of ethidiumbromide kills all CNS glia (oligodendrocytes and astrocytes), but leaves axons...
owned by Thermo Fisher Scientific SYBR Gold is more sensitive than ethidiumbromide, SYBR Green I, and SYBR Green II for detecting various types of nucleic...
chloride in water. DNA will go to the density that reflects its own, and ethidiumbromide is then added to enhance the visuals the nucleic acid band provides...
marketed as a safer alternative to ethidiumbromide. However, as the molecule itself is quite a bit larger than ethidiumbromide, it does not bind to the column...
begin, UV light is shone on the gel in order to illuminate all the ethidiumbromide-stained DNA. Care must be taken to avoid exposing the DNA to mutagenic...
other applications. The DNA is normally visualized by staining with ethidiumbromide and then viewed under a UV light, but other methods of staining are...
color under UV light. Ethidiumbromide binds nucleic acid chains through the process of Intercalation. While Ethidiumbromide is a popular stain it is...
the RNA to limit secondary structure. The gels can be stained with ethidiumbromide (EtBr) and viewed under UV light to observe the quality and quantity...
detection: the compound ethidiumbromide, in aqueous solution, has very little fluorescence, as it is quenched by water. Ethidiumbromide's fluorescence is greatly...
gel can be stained to make them visible. DNA may be visualized using ethidiumbromide which, when intercalated into DNA, fluoresce under ultraviolet light...
using either ethidiumbromide or SYBR Green dyes.[citation needed] The most common electrophoretic stain in agarose gel is ethidiumbromide, however, SYBR...
a restriction enzyme, running it on an agarose gel, staining with ethidiumbromide (EtBr) or a different stain and comparing the intensity of the DNA...
labeling, protein labeling, and genetic labeling are widely utilized. Ethidiumbromide, fluorescein and green fluorescent protein are common tags. The most...
dimidium bromide and its derivatives were first used in 1948 in animal cases in Africa, and became known as homidium (or as ethidiumbromide in molecular...
Ethidiumbromide-stained PCR products after gel electrophoresis. Two sets of primers were used to amplify a target sequence from three different tissue...
trypanocidal agent used in veterinary medicine. It consists of a single ethidiumbromide like subunit linked to a fragment of the diminazene molecule.[citation...
depleted of their own mitochondrial DNA by prolonged incubation with ethidiumbromide, a chemical which inhibits mitochondrial DNA replication. The rho-zero...
that bind to nucleic acids and selectively fluoresce when bound (e.g. Ethidiumbromide). This method is useful for cases where concentration is too low to...
dispensed circular well in an agarose gel layer, in which DNA stained by ethidiumbromide is uniformly distributed. After the incubation, a circular dark zone...
SAFETY DATA SHEET. kpl.com Ohta T, Tokishita S, Yamagata H (2001). "Ethidiumbromide and SYBR Green I enhance the genotoxicity of UV-irradiation and chemical...
compounds and are known or suspected carcinogens. Examples include ethidiumbromide and acridine.[citation needed] Mismatched base pairs can be generated...
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