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Chemical compound
Acridine orange is an organic compound that serves as a nucleic acid-selective fluorescent dye with cationic properties useful for cell cycle determination. Acridine orange is cell-permeable, which allows the dye to interact with DNA by intercalation, or RNA via electrostatic attractions. When bound to DNA, acridine orange is very similar spectrally to an organic compound known as fluorescein. Acridine orange and fluorescein have a maximum excitation at 502nm and 525 nm (green). When acridine orange associates with RNA, the fluorescent dye experiences a maximum excitation shift from 525 nm (green) to 460 nm (blue). The shift in maximum excitation also produces a maximum emission of 650 nm (red). Acridine orange is able to withstand low pH environments, allowing the fluorescent dye to penetrate acidic organelles such as lysosomes and phagolysosomes that are membrane-bound organelles essential for acid hydrolysis or for producing products of phagocytosis of apoptotic cells. Acridine orange is used in epifluorescence microscopy and flow cytometry. The ability to penetrate the cell membranes of acidic organelles and cationic properties of acridine orange allows the dye to differentiate between various types of cells (i.e., bacterial cells and white blood cells). The shift in maximum excitation and emission wavelengths provides a foundation to predict the wavelength at which the cells will stain.[1]
^Yektaeian, Narjes; Mehrabani, Davood; Sepaskhah, Mozhdeh; Zare, Shahrokh; Jamhiri, Iman; Hatam, Gholamreza (December 2019). "Lipophilic tracer Dil and fluorescence labeling of acridine orange used for Leishmania major tracing in the fibroblast cells". Heliyon. 5 (12): e03073. Bibcode:2019Heliy...503073Y. doi:10.1016/j.heliyon.2019.e03073. PMC 6928280. PMID 31890980.
Acridineorange is an organic compound that serves as a nucleic acid-selective fluorescent dye with cationic properties useful for cell cycle determination...
acridines; at one time acridine dyes were popular, but they are now relegated to niche applications, such as with acridineorange. The name is a reference...
tissues; vital dyes (suitable for use with living organisms) are noted. Acridineorange (AO) is a nucleic acid selective fluorescent cationic dye useful for...
special structure, the fluorescent mitochondrial indicator, nonyl acridineorange (NAO) was introduced in 1982, and was later found to target mitochondria...
hydrochloride salt. Acridine yellow damages DNA and is used as a mutagen in microbiology. Acridine yellow is similar to acridineorange. According to a publication...
parasites. The blood is taken in a QBC capillary tube which is coated with acridineorange (a fluorescent dye) and centrifuged; the fluorescing parasitized erythrocytes...
metachromasia. Another example of metachromatic dye (fluorochrome) is acridineorange. Under certain conditions it stains single-stranded nucleic acids fluorescing...
the atomic symbol for the misidentified chemical element Ausenium. Acridineorange, a fluorescent dye Authorship obfuscation, a privacy technique Ao (surname)...
susceptibility to denaturation at low pH using the metachromatic dye acridineorange, reveals the G1Q, G1A, and G1B cell cycle compartments and also makes...
in malaria patients along the Thai-Myanmar border, as revealed by acridineorange staining and PCR-based diagnoses". Tropical Medicine and International...
apoptosis is tested by treating the lysosomal membrane with acridineorange. Acridineorange radiates a red fluorescent color at an increased concentration...
011. PMID 19682624. Hathaway WE, Newby LA, Githens JH (1964). "The AcridineOrange Viability Test Applied to Bone Marrow Cells. I. Correlation with Trypan...
coworkers first produced electroluminescence in crystalline thin films of acridineorange and quinacrine. In 1960, researchers at Dow Chemical developed AC-driven...
Gram stain, Giemsa stain, H&E stain, Warthin-Starry silver stain, acridineorange stain, and phase-contrast microscopy. It is capable of forming biofilms...
benzo[a]pyrene and dibenzo[a,i]pyrene. Chlorophyllin also binds to acridineorange. Chlorophyllin has been validated to exhibit ameliorative effects against...
Observatory New Age Outlaws, a wrestling tag team Sodium oxide (NaO) Nonyl AcridineOrange, a fluorescent die. It can stain cardiolipin content of mitochondria...
They applied high alternating voltages in air to materials such as acridineorange dye, either deposited on or dissolved in cellulose or cellophane thin...
molecular formula C17H19N3 (molar mass : 265.35 g/mol) may refer to: Acridineorange Antazoline Esmirtazapine Mirtazapine This set index page lists chemical...
genetics for agarose gel DNA electrophoresis. GelGreen consists of two acridineorange subunits that are bridged by a linear oxygenated spacer. Its fluorophore...
parasites. The blood is taken in a QBC capillary tube which is coated with acridineorange (a fluorescent dye) and centrifuged; the fluorescing parasites can...
list is uptodate as of January 2024. Acenaphthene Acepyrene Aciclovir Acridineorange Acriflavinium chloride Acrylic acid Acrylic fibres Acrylonitrile-butadiene-styrene...
previously in PDT) include acridineorange, methylene blue, curcumin, and indocyanine green. A study by Suzuki et al. used acridineorange, a fluorescent cationic...
Joshua Lederberg and colleagues) and then in 1985 of the F plasmid by acridineorange curing.[citation needed] Strains derived from MG1655 include DH1, parent...
that is identified through the lens. Fluorescent dyes include DAPI, AcridineOrange, SYBR Green 1, and YO-PRO-1, all of which are capable of staining both...
treatment opens up defective sperm DNA at the sites of damage. Through acridineorange (AO) staining, AO molecules are intercalated into double-stranded DNA...
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