A vital stain in a casual usage may mean a stain that can be applied on living cells without killing them. Vital stains have been useful for diagnostic and surgical techniques in a variety of medical specialties.[1] In supravital staining, living cells have been removed from an organism, whereas intravital staining is done by injecting or otherwise introducing the stain into the body. The term vital stain is used by some authors to refer to an intravital stain, and by others interchangeably with a supravital stain, the core concept being that the cell being examined is still alive. In a more strict sense, the term vital staining has a meaning contrasting with supravital staining. While in supravital staining the living cells take up the stain, in "vital staining" – the most accepted but apparently paradoxical meaning of this term, the living cells exclude the stain i.e. stain negatively and only the dead cells stain positively and thus viability can be assessed by counting the percentage of total cells that stain negatively. Very bulky or highly charged stains that don't cross live plasma membrane are used as vital stains and supravital stains are those that are either small or are pumped actively into live cells. Since supravital and intravital nature of the staining depends on the dye, a combination of supravital and vital dyes can also be used in a sophisticated way to better classify cells into distinct subsets (e.g. viable, dead, dying etc.).
^Rodrigues EB, Costa EF, Penha FM, Melo GB, Bottós J, Dib E, Furlani B, Lima VC, Maia M, Meyer CH, Höfling-Lima AL, Farah ME (2009). "The use of vital dyes in ocular surgery". Survey of Ophthalmology. 54 (5): 576–617. doi:10.1016/j.survophthal.2009.04.011. PMID 19682624.
A vitalstain in a casual usage may mean a stain that can be applied on living cells without killing them. Vitalstains have been useful for diagnostic...
domain structures of block copolymers. In vivo staining (also called vitalstaining or intravital staining) is the process of dyeing living tissues. By...
term "vitalstain" is used by some authors to refer specifically to an intravital stain, and by others interchangeably with a supravital stain, the core...
the term "vitalstaining" means the polar opposite of "supravital staining." If living cells absorb the stain during supravital staining, they exclude...
of specialised cells that effectively clear colloidal vitalstains (so called because they stain living cells) from the blood circulation. The term is...
is a direct dye for cotton textiles. In biosciences, it is used as a vitalstain to selectively colour dead tissues or cells blue. Live cells or tissues...
differentiate bacteria for lactose fermentation. Neutral red can be used as a vitalstain. The Neutral Red Cytotoxicity Assay was first developed by Ellen Borenfreund...
Janus Green B is a basic dye and vitalstain used in histology. It is also used to stain mitochondria supravitally, as was introduced by Leonor Michaelis...
essential to an individual's life Vital signs, medical signs that indicate life-sustaining bodily functions Vitalstain, a stain that can be applied on living...
(oxygen) and can be fixed by immersion of the stained specimen in an aqueous solution of ammonium molybdate. Vital methylene blue was formerly much used for...
manufactured from aniline. Aniline Yellow is used in microscopy for vitalstaining, in pyrotechnics for yellow colored smokes, in yellow pigments and inks...
in the EU and Australia and New Zealand. Green S is a vital dye, meaning it can be used to stain living cells. It is used in ophthalmology, along with...
(antibodies, peptides, nucleic acids). Fluorophores are notably used to stain tissues, cells, or materials in a variety of analytical methods, such as...
basic red 2) is a biological stain used in histology and cytology. Safranin is used as a counterstain in some staining protocols, colouring cell nuclei...
organic compound of the thiazine class of heterocycles. It is used as a stain and as an antimicrobial agent. It is classified as an azine dye, and the...
Nile blue (or Nile blue A) is a stain used in biology and histology. It may be used with live or fixed cells, and imparts a blue colour to cell nuclei...
between RNA and DNA staining. PI is widely used in fluorescence staining and visualization of the plant cell wall. Viability assay Vitalstain SYBR Green I Ethidium...
is vital that non-staining drinks or foods are eaten during this time as enamel is prone to adhere to stains. Drink fluids that may cause staining through...
Nile red (also known as Nile blue oxazone) is a lipophilic stain. Nile red stains intracellular lipid droplets yellow. In most polar solvents, Nile red...
Acridine orange staining has to be performed at an acidic pH to obtain the differential staining, which allows bacterial cells to stain orange and tissue...
(vitalstain) Propidium iodide, DNA stain that can differentiate necrotic, apoptotic and normal cells. Resazurin TUNEL assay Cytotoxicity Vitalstain Welch...
"Die vitale Farbung, eine Darstellungsmethode der Zellgranula" [The vitalstain: a method of imaging cell granules]. Archiv für Mikroskopische Anatomie...
acid-Schiff stain to stain cellulose, and in Feulgen stain to stain DNA. It was more common in the past; today it is partially replaced by other stains. It has...
re-investigation of the original vitalstain experiments carried out 100–140 years ago it was concluded that the vitalstain accumulated mainly in LSECs....
ulcers show a typical dendritic pattern of staining. Rose-Bengal dye is also used for supra-vitalstaining purposes, but it may be very irritating to...
red blood cells'. Giemsa stain is used to distinguish all three types of blood smears. The young cells will generally stain gray or blue in the cytoplasm...
covalently binds to lysine residues and other amine sources like CFSE. If a stained cell divides, the dye is divided equally between the two daughter cells...
(3,3′-dihexyloxacarbocyanine iodide) is a fluorescent dye used for the staining of a cell's endoplasmic reticulum, vesicle membranes and mitochondria....
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