Flow cytometry (FC) is a technique used to detect and measure the physical and chemical characteristics of a population of cells or particles.[1][2][3][4]
In this process, a sample containing cells or particles is suspended in a fluid and injected into the flow cytometer instrument. The sample is focused to ideally flow one cell at a time through a laser beam, where the light scattered is characteristic to the cells and their components. Cells are often labeled with fluorescent markers so light is absorbed and then emitted in a band of wavelengths. Tens of thousands of cells can be quickly examined and the data gathered are processed by a computer.[5]
Flow cytometry is routinely used in basic research, clinical practice, and clinical trials. Uses for flow cytometry include:
Cell counting
Cell sorting
Determining cell characteristics and function
Detecting microorganisms
Biomarker detection
Protein engineering detection
Diagnosis of health disorders such as blood cancers
Measuring genome size
A flow cytometry analyzer is an instrument that provides quantifiable data from a sample. Other instruments using flow cytometry include cell sorters which physically separate and thereby purify cells of interest based on their optical properties.
^Picot J, Guerin CL, Le Van Kim C, Boulanger CM (March 2012). "Flow cytometry: retrospective, fundamentals and recent instrumentation". Cytotechnology. 64 (2): 109–30. doi:10.1007/s10616-011-9415-0. PMC 3279584. PMID 22271369.
^Shapiro HM (2003). Practical flow cytometry (4th ed.). New York: Wiley-Liss. ISBN 978-0-471-41125-3.
^Givan AL (2011). "Flow Cytometry: An Introduction". In Hawley T, Hawley R (eds.). Flow Cytometry Protocols. Methods in Molecular Biology. Vol. 699. Humana Press. pp. 1–29. doi:10.1007/978-1-61737-950-5_1. ISBN 978-1-61737-949-9. PMID 21116976.
Flowcytometry (FC) is a technique used to detect and measure the physical and chemical characteristics of a population of cells or particles. In this...
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arrest with a G2 or M phase DNA content, which can be verified with flowcytometry. From microscopy it has been determined they do enter mitosis but they...
enumeration (cell counting) by direct and individual (microscopic, flowcytometry), direct and bulk (biomass), indirect and individual (colony counting)...
studies have attempted to develop systems for real-time detection via flowcytometry or microscopy of airborne sporangia collected in air samplers. Whilst...
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