Global Information Lookup Global Information

Discontinuous electrophoresis information


DNA bands after electrophoresis

Discontinuous electrophoresis (colloquially disc electrophoresis[a]) is a type of polyacrylamide gel electrophoresis. It was developed by Ornstein and Davis.[2][1] This method produces high resolution and good band definition. It is widely used technique for separating proteins according to size and charge.[3]

  1. ^ a b Ornstein, Leonard (2006-12-16). "Disc Electrophoresis-I Background and Theory". Annals of the New York Academy of Sciences. 121 (2). Wiley: 321–349. doi:10.1111/j.1749-6632.1964.tb14207.x. ISSN 0077-8923. PMID 14240533. S2CID 28591995.
  2. ^ Williams, Donald E. & Reisfeld, Ralph A. (1964). "DISC Electrophoresis IN POLYACRYLAMIDE GELS". Annals of the New York Academy of Sciences. 121 (2): 372–381. Bibcode:1964NYASA.121..373W. doi:10.1111/j.1749-6632.1964.tb14210.x. PMID 14240536. S2CID 46152998.
  3. ^ PolyAcrylamide Gel Electrophoresis (PDF). davidson.edu.


Cite error: There are <ref group=lower-alpha> tags or {{efn}} templates on this page, but the references will not show without a {{reflist|group=lower-alpha}} template or {{notelist}} template (see the help page).

and 22 Related for: Discontinuous electrophoresis information

Request time (Page generated in 0.8249 seconds.)

Discontinuous electrophoresis

Last Update:

Discontinuous electrophoresis (colloquially disc electrophoresis) is a type of polyacrylamide gel electrophoresis. It was developed by Ornstein and Davis...

Word Count : 417

Gel electrophoresis

Last Update:

large to sieve proteins. Gel electrophoresis can also be used for the separation of nanoparticles. Gel electrophoresis uses a gel as an anticonvective...

Word Count : 5057

Gel electrophoresis of proteins

Last Update:

Protein electrophoresis is a method for analysing the proteins in a fluid or an extract. The electrophoresis may be performed with a small volume of sample...

Word Count : 2351

Isotachophoresis

Last Update:

of zone electrophoresis. tITP is nowadays more widespread than conventional ITP because it is easily implemented in capillary electrophoresis (CE) separations...

Word Count : 580

Leonard Ornstein

Last Update:

Utrecht, Netherlands Alma mater University of Leiden Known for Discontinuous electrophoresis Ornstein-Zernike equation Ornstein-Uhlenbeck process Scientific...

Word Count : 704

MOPS

Last Update:

PMID 5942950. Thomas, J; Hodes, ME (1981). "A new discontinuous buffer system for the electrophoresis of cationic proteins at near-neutral pH". Analytical...

Word Count : 268

Sodium dodecyl sulfate

Last Update:

isolated genetic material, and for denaturing proteins in preparation for electrophoresis in the SDS-PAGE technique. In the case of SDS-PAGE, the compound works...

Word Count : 3064

Surface charge

Last Update:

example is electrophoresis, where a charged particle suspended in a media will move as a result of an applied electrical field. Electrophoresis is widely...

Word Count : 2327

Microsatellite

Last Update:

been amplified, they are resolved either through gel electrophoresis or capillary electrophoresis, which will allow the analyst to determine how many repeats...

Word Count : 7384

Electrospray ionization

Last Update:

electrospray-LCMS spectra and/or nanoESI-MS spectra. have been studied. Capillary electrophoresis-mass spectrometry was enabled by an ESI interface that was developed...

Word Count : 5340

Beta thalassemia

Last Update:

protein levels. In thalassemia, an additional, contiguous length or a discontinuous fragment of non-coding instructions is included in the mRNA. This happens...

Word Count : 4880

Plasmapheresis

Last Update:

method having its own advantages and disadvantages:[citation needed] Discontinuous flow centrifugation: One venous catheter line is required. Typically...

Word Count : 3057

Antibody

Last Update:

trimers, etc.), and to bind effector molecules more easily. In an electrophoresis test of blood proteins, antibodies mostly migrate to the last, gamma...

Word Count : 13287

Apoptosis

Last Update:

These give a characteristic "laddered" appearance on agar gel after electrophoresis. Tests for DNA laddering differentiate apoptosis from ischemic or toxic...

Word Count : 10629

Apoptotic DNA fragmentation

Last Update:

acridine orange shows that DNA fragmentation within individual cells is discontinuous likely reflecting different levels of restriction in accessibility of...

Word Count : 2222

Fluorescence

Last Update:

useful in visualising the location of DNA fragments in agarose gel electrophoresis. Intercalated ethidium is in a hydrophobic environment when it is between...

Word Count : 10883

Miniature mass spectrometer

Last Update:

separation tools like gas chromatography, liquid chromatography or electrophoresis to reduce the effect of the matrix or background and improve the selectivity...

Word Count : 3321

Sperm sorting

Last Update:

flow cytometry. Density gradient centrifugation (in a continuous or discontinuous gradient) can concentrate semen samples with low concentration of sperm...

Word Count : 2187

History of molecular theory

Last Update:

53 (4): 1367–1400. doi:10.1021/ja01355a027. Perrin, Jean, B. (1926). Discontinuous Structure of Matter, Nobel Lecture, December 11. Mitch Jacoby, "Atomic...

Word Count : 4474

Piet Borst

Last Update:

mitochondrial DNA, but must be imported. Development of ethidium-agarose electrophoresis (5) (for the separation of DNA topoisomers); proof that linear DNA...

Word Count : 3526

Microthermoforming

Last Update:

(thermo)forming process is used to fabricate film microchips for capillary electrophoresis (CE) [3–5] and for three-dimensional cell cultivation [6–8]. The process...

Word Count : 1193

Physiological effects in space

Last Update:

1999). "Enhanced protein electrophoresis technique for separating human skeletal muscle myosin heavy chain isoforms". Electrophoresis. 20 (3): 466–8. doi:10...

Word Count : 21808

PDF Search Engine © AllGlobal.net