Discontinuous electrophoresis (colloquially disc electrophoresis[a]) is a type of polyacrylamide gel electrophoresis. It was developed by Ornstein and Davis.[2][1] This method produces high resolution and good band definition. It is widely used technique for separating proteins according to size and charge.[3]
^ abOrnstein, Leonard (2006-12-16). "Disc Electrophoresis-I Background and Theory". Annals of the New York Academy of Sciences. 121 (2). Wiley: 321–349. doi:10.1111/j.1749-6632.1964.tb14207.x. ISSN 0077-8923. PMID 14240533. S2CID 28591995.
^Williams, Donald E. & Reisfeld, Ralph A. (1964). "DISC Electrophoresis IN POLYACRYLAMIDE GELS". Annals of the New York Academy of Sciences. 121 (2): 372–381. Bibcode:1964NYASA.121..373W. doi:10.1111/j.1749-6632.1964.tb14210.x. PMID 14240536. S2CID 46152998.
^PolyAcrylamide Gel Electrophoresis(PDF). davidson.edu.
Cite error: There are <ref group=lower-alpha> tags or {{efn}} templates on this page, but the references will not show without a {{reflist|group=lower-alpha}} template or {{notelist}} template (see the help page).
and 22 Related for: Discontinuous electrophoresis information
Discontinuouselectrophoresis (colloquially disc electrophoresis) is a type of polyacrylamide gel electrophoresis. It was developed by Ornstein and Davis...
large to sieve proteins. Gel electrophoresis can also be used for the separation of nanoparticles. Gel electrophoresis uses a gel as an anticonvective...
Protein electrophoresis is a method for analysing the proteins in a fluid or an extract. The electrophoresis may be performed with a small volume of sample...
of zone electrophoresis. tITP is nowadays more widespread than conventional ITP because it is easily implemented in capillary electrophoresis (CE) separations...
Utrecht, Netherlands Alma mater University of Leiden Known for Discontinuouselectrophoresis Ornstein-Zernike equation Ornstein-Uhlenbeck process Scientific...
PMID 5942950. Thomas, J; Hodes, ME (1981). "A new discontinuous buffer system for the electrophoresis of cationic proteins at near-neutral pH". Analytical...
isolated genetic material, and for denaturing proteins in preparation for electrophoresis in the SDS-PAGE technique. In the case of SDS-PAGE, the compound works...
example is electrophoresis, where a charged particle suspended in a media will move as a result of an applied electrical field. Electrophoresis is widely...
been amplified, they are resolved either through gel electrophoresis or capillary electrophoresis, which will allow the analyst to determine how many repeats...
electrospray-LCMS spectra and/or nanoESI-MS spectra. have been studied. Capillary electrophoresis-mass spectrometry was enabled by an ESI interface that was developed...
protein levels. In thalassemia, an additional, contiguous length or a discontinuous fragment of non-coding instructions is included in the mRNA. This happens...
method having its own advantages and disadvantages:[citation needed] Discontinuous flow centrifugation: One venous catheter line is required. Typically...
trimers, etc.), and to bind effector molecules more easily. In an electrophoresis test of blood proteins, antibodies mostly migrate to the last, gamma...
These give a characteristic "laddered" appearance on agar gel after electrophoresis. Tests for DNA laddering differentiate apoptosis from ischemic or toxic...
acridine orange shows that DNA fragmentation within individual cells is discontinuous likely reflecting different levels of restriction in accessibility of...
useful in visualising the location of DNA fragments in agarose gel electrophoresis. Intercalated ethidium is in a hydrophobic environment when it is between...
separation tools like gas chromatography, liquid chromatography or electrophoresis to reduce the effect of the matrix or background and improve the selectivity...
flow cytometry. Density gradient centrifugation (in a continuous or discontinuous gradient) can concentrate semen samples with low concentration of sperm...
53 (4): 1367–1400. doi:10.1021/ja01355a027. Perrin, Jean, B. (1926). Discontinuous Structure of Matter, Nobel Lecture, December 11. Mitch Jacoby, "Atomic...
mitochondrial DNA, but must be imported. Development of ethidium-agarose electrophoresis (5) (for the separation of DNA topoisomers); proof that linear DNA...
(thermo)forming process is used to fabricate film microchips for capillary electrophoresis (CE) [3–5] and for three-dimensional cell cultivation [6–8]. The process...
1999). "Enhanced protein electrophoresis technique for separating human skeletal muscle myosin heavy chain isoforms". Electrophoresis. 20 (3): 466–8. doi:10...