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Gel electrophoresis of proteins information


Proteins separated by SDS-PAGE, Coomassie brilliant blue staining

Protein electrophoresis is a method for analysing the proteins in a fluid or an extract. The electrophoresis may be performed with a small volume of sample in a number of alternative ways with or without a supporting medium, namely agarose or polyacrylamide. Variants of gel electrophoresis include SDS-PAGE, free-flow electrophoresis, electrofocusing, isotachophoresis, affinity electrophoresis, immunoelectrophoresis, counterelectrophoresis, and capillary electrophoresis. Each variant has many subtypes with individual advantages and limitations. Gel electrophoresis is often performed in combination with electroblotting or immunoblotting to give additional information about a specific protein.[1]

  1. ^ Michov, Budin (2022). Electrophoresis Fundamentals: Essential Theory and Practice. De Gruyter. p. 490. doi:10.1515/9783110761641. ISBN 9783110761641. S2CID 247987700.

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Gel electrophoresis of proteins

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(EMSA) or mobility shift electrophoresis, also referred as a gel shift assay, gel mobility shift assay, band shift assay, or gel retardation assay, is a...

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ligand-binding proteins, one-dimensional electrophoresis similar to counter electrophoresis or to "rocket immunoelectrophoresis", affinity electrophoresis may be...

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Immunoelectrophoresis

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gel, but are stained with protein stains like Coomassie brilliant blue in the dried gel. In contrast to SDS-gel electrophoresis, the electrophoresis in...

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or by SDS-PAGE (sodium dodecyl sulfate-polyacrylamide gel electrophoresis) analysis. Proteins are often purified by using 2D-PAGE and are then analysed...

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needed for protein sites with low density. High-abundance proteins are frequently found beside low-abundance proteins on 2DE gels. Because of the fluorescent...

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refers to capillary zone electrophoresis (CZE), but other electrophoretic techniques including capillary gel electrophoresis (CGE), capillary isoelectric...

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Western blot

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preparation, the protein content is ready to be separated by the utilization of gel electrophoresis. The proteins of the sample are separated using gel electrophoresis...

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Agarose

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for the separation of large molecules, especially DNA, by electrophoresis. Slabs of agarose gels (usually 0.7 - 2%) for electrophoresis are readily prepared...

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proteins, sometimes referred to as blood proteins, are proteins present in blood plasma. They serve many different functions, including transport of lipids...

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Electrophoretic color marker

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monitor the progress of agarose gel electrophoresis and polyacrylamide gel electrophoresis (PAGE) since DNA, RNA, and most proteins are colourless. The...

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Electroblotting

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to transfer proteins or nucleic acids onto a membrane by using PVDF or nitrocellulose, after gel electrophoresis. The protein or nucleic acid can...

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proteins are reduced and cysteine amino acids are carbamidomethylated chemically or acrylamidated during the gel electrophoresis. Then the proteins are...

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a type of zone electrophoresis usually performed on proteins in a gel that takes advantage of the fact that overall charge on the molecule of interest...

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