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Antibody microarray information


Samples of antibody microarray creations and detections.

An antibody microarray (also known as antibody array) is a specific form of protein microarray. In this technology, a collection of captured antibodies are spotted and fixed on a solid surface such as glass, plastic, membrane, or silicon chip, and the interaction between the antibody and its target antigen is detected. Antibody microarrays are often used for detecting protein expression from various biofluids including serum, plasma and cell or tissue lysates. Antibody arrays may be used for both basic research and medical and diagnostic applications.[1][2][3][4]

  1. ^ Rivas LA, García-Villadangos M, Moreno-Paz M, Cruz-Gil P, Gómez-Elvira J, Parro V (November 2008). "A 200-antibody microarray biochip for environmental monitoring: searching for universal microbial biomarkers through immunoprofiling". Anal. Chem. 80 (21): 7970–9. doi:10.1021/ac8008093. PMID 18837515.
  2. ^ Chaga GS (2008). "Antibody Arrays for Determination of Relative Protein Abundances". Tissue Proteomics. Methods in Molecular Biology. Vol. 441. pp. 129–51. doi:10.1007/978-1-60327-047-2_9. ISBN 978-1-58829-679-5. PMID 18370316.
  3. ^ Wilson J. J.; Burgess R.; Mao Y. Q.; Luo S.; Tang H.; Jones V. S.; et al. (2015). Chapter Seven-Antibody Arrays in Biomarker Discovery. Vol. 69. pp. 255–324. doi:10.1016/bs.acc.2015.01.002. ISBN 9780128022658. PMID 25934364. {{cite book}}: |journal= ignored (help)
  4. ^ Lin Y., Huang R.C., Cao X., Wang S.-M., Shi Q., Huang R.-P. (2003). "Detection of multiple cytokines by protein arrays from cell lysate and tissue lysate". Clin Chem Lab Med. 41 (2): 139–145. doi:10.1515/cclm.2003.023. PMID 12666998. S2CID 34616684.{{cite journal}}: CS1 maint: multiple names: authors list (link)

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Antibody microarray

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are immobilized on individual spots on a microarray that is then incubated with a single specific antibody to detect expression of the target protein...

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microarrays are often used to profile an enzyme (like kinase, phosphatase, protease, acetyltransferase, histone deacetylase etc.), to map an antibody...

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This microarray format is very similar to DNA microarray, protein microarray and antibody microarray. In chemical genetics research, they are routinely...

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secreted by human chondrocytes in culture and serum contaminants. An antibody microarray is a highly sensitive and high-throughput method for protein detection...

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antigens (proteins) in cells and tissue, by exploiting the principle of antibodies binding specifically to antigens in biological tissues. Albert Hewett...

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Cellular microarray

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A cellular microarray (or cell microarray) is a laboratory tool that allows for the multiplex interrogation of living cells on the surface of a solid support...

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Immunoassay

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macromolecule or a small molecule in a solution through the use of an antibody (usually) or an antigen (sometimes). The molecule detected by the immunoassay...

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types of microarrays have been created to specifically observe the immune system response and interactions. Antibody microarrays use antibodies as probes...

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Pérez-Mercader, Juan; Gómez-Elvira, Javier (2011). "SOLID3: A Multiplex Antibody Microarray-Based Optical Sensor Instrument forIn SituLife Detection in Planetary...

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and replication of DNA. In addition, other kinds of proteins include antibodies that protect an organism from infection, and hormones that send important...

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the human genome, with these fragments being used as probes on the DNA microarray. Now probes of various origins such as cDNA, genomic PCR products and...

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Gómez-Elvira (2012). "THE SOLID3 ("SIGNS OF LIFE DETECTOR") INSTRUMENT: AN ANTIBODY MICROARRAY-BASED BIOSENSOR FOR PLANETARY EXPLORATION" (PDF). Concepts and Approaches...

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Oligonucleotide

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DNA microarrays are a useful analytical application of oligonucleotides. Compared to standard cDNA microarrays, oligonucleotide based microarrays have...

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Western blot

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the primary antibody, before excess antibody is washed off. A secondary antibody is added which recognizes and binds to the primary antibody. The secondary...

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Tanox

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pioneer in the antibody field. “Antibody matrix” methodology and applications, which focused on antibody microarrays and laid out the microarray concept generally...

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protein microarrays with automated systems that print the slides and record results. Microarrays of cell analytes, arrays of cell lysate, antibody microarrays...

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industry. His early research involving the Immunoglobulin E (IgE) pathway and antibody-based therapeutics lead to the development of omalizumab (also known as...

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influenza pandemic. In 2011, researchers reported the discovery of an antibody effective against all types of the influenza A virus. Influenza A virus...

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