To study binding properties, specificity and functionality and kinetics of protein-peptide or protein-protein interactions
A peptide microarray (also commonly known as peptide chip or peptide epitope microarray) is a collection of peptides displayed on a solid surface, usually a glass or plastic chip. Peptide chips are used by scientists in biology, medicine and pharmacology to study binding properties and functionality and kinetics of protein-protein interactions in general. In basic research, peptide microarrays are often used to profile an enzyme (like kinase, phosphatase, protease, acetyltransferase, histone deacetylase etc.), to map an antibody epitope or to find key residues for protein binding. Practical applications are seromarker discovery, profiling of changing humoral immune responses of individual patients during disease progression, monitoring of therapeutic interventions, patient stratification and development of diagnostic tools and vaccines.
An example of a peptide array used for epitope identification and specificity mapping.[1]
^Hansen, Lajla Bruntse; Buus, Soren; Schafer-Nielsen, Claus (2013-07-23). "Identification and Mapping of Linear Antibody Epitopes in Human Serum Albumin Using High-Density Peptide Arrays". PLOS ONE. 8 (7): e68902. Bibcode:2013PLoSO...868902H. doi:10.1371/journal.pone.0068902. ISSN 1932-6203. PMC 3720873. PMID 23894373.
and 24 Related for: Peptide microarray information
A peptidemicroarray (also commonly known as peptide chip or peptide epitope microarray) is a collection of peptides displayed on a solid surface, usually...
use of protein, peptide and carbohydrate microarrays is expanding. Types of microarrays include: DNA microarrays, such as cDNA microarrays, oligonucleotide...
A protein microarray (or protein chip) is a high-throughput method used to track the interactions and activities of proteins, and to determine their function...
chemistry, peptide synthesis is the production of peptides, compounds where multiple amino acids are linked via amide bonds, also known as peptide bonds....
PMHC cellular microarrays are a type of cellular microarray that has been spotted with pMHC complexes peptide-MHC class I or peptide-MHC class II. These...
Jerini's subsidiaries Jerini US Inc. and JPT Peptide Technologies GmbH, provider of peptides and peptidemicroarrays, were acquired by Theracode GmbH, Mainz...
A cellular microarray (or cell microarray) is a laboratory tool that allows for the multiplex interrogation of living cells on the surface of a solid support...
concentrations for which the antibody probes are specific. Peptidemicroarrays use antigen peptides as probes and serum antibodies as targets. These can be...
identification of proteins and sequencing of peptides (most often after in-gel digestion), protein microarrays, which allow the detection of the relative...
of c-Src tyrosine kinase substrates using mass spectrometry and peptidemicroarrays". Journal of Proteome Research. 7 (9): 3900–10. doi:10.1021/pr800198w...
A reverse phase protein lysate microarray (RPMA) is a protein microarray designed as a dot-blot platform that allows measurement of protein expression...
biomarker identification, and many more. Mass spectrometry and microarray produce peptide fragmentation information but do not give identification of specific...
numbers per cell. A drawback that separates it from microarray analysis is its limited scope: a microarray can handle parallel analysis of multiple genes while...
in immunoproteomics is carried out by techniques including gel based, microarray based, and DNA based techniques, with mass spectroscopy typically being...
used to determine the genes implicated in a disorder: one might compare microarray data from cancerous epithelial cells to data from non-cancerous cells...
Combinatorial chemistry can be used for the synthesis of small molecules and for peptides. Strategies that allow identification of useful components of the libraries...
important in certain molecular biology applications. For example, in microarray expression technologies, it is important to know which strand is "viewed"...
like phycoerythrin are used in a number of modern immunoassays. Protein microarrays are a type of immunoassay that often employ fluorogenic reporters. Some...
melanin-activating peptide receptor, or melanotropin receptor, is a G protein–coupled receptor that binds to a class of pituitary peptide hormones known as...
cancer therapy to target specific chemotherapy. (See RNA-Seq and DNA_microarray for details.) Similarly, the analysis of the location of protein expression...
Global Information