Main staining patterns on chromogenic immunohistochemistry.Immunofluorescence of human skin using an anti-IgA antibody. The skin is from a patient with Henoch–Schönlein purpura: IgA deposits are found in the walls of small superficial capillaries (yellow arrows). The pale wavy green area on top is the epidermis, the bottom fibrous area is the dermis.
Immunohistochemistry (IHC) is a form of immunostaining. It involves the process of selectively identifying antigens (proteins) in cells and tissue, by exploiting the principle of antibodies binding specifically to antigens in biological tissues. Albert Hewett Coons, Ernest Berliner, Norman Jones and Hugh J Creech was the first to develop immunofluorescence in 1941. This led to the later development of immunohistochemistry.[1][2]
Immunohistochemical staining is widely used in the diagnosis of abnormal cells such as those found in cancerous tumors. In some cancer cells certain tumor antigens are expressed which make it possible to detect. Immunohistochemistry is also widely used in basic research, to understand the distribution and localization of biomarkers and differentially expressed proteins in different parts of a biological tissue.[3]
^Ortiz Hidalgo C (2022), Del Valle L (ed.), "Immunohistochemistry in Historical Perspective: Knowing the Past to Understand the Present", Immunohistochemistry and Immunocytochemistry, Methods in Molecular Biology, vol. 2422, New York, NY: Springer US, pp. 17–31, doi:10.1007/978-1-0716-1948-3_2, ISBN 978-1-0716-1947-6, PMID 34859396, S2CID 244861186, retrieved 2024-02-22
^"Immunohistochemistry: Origins, Tips, and a Look to the Future". The Scientist Magazine®. Retrieved 2024-02-22.
^Duraiyan J, Govindarajan R, Kaliyappan K, Palanisamy M (2012). "Applications of immunohistochemistry". Journal of Pharmacy and Bioallied Sciences. 4 (6): S307-9. doi:10.4103/0975-7406.100281. ISSN 0975-7406. PMC 3467869. PMID 23066277.
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Immunohistochemistry (IHC) is a form of immunostaining. It involves the process of selectively identifying antigens (proteins) in cells and tissue, by...
compartments are expressing the antigen. Immunocytochemistry differs from immunohistochemistry in that the former is performed on samples of intact cells that have...
embedding media, but acrylic resins are also used, particularly where immunohistochemistry is required. For tissues to be cut in a frozen state, tissues are...
needle biopsy, vacuum-assisted breast biopsy, or surgical excision. Immunohistochemistry (IHC) is generally used to measure the amount of HER2 protein present...
and molecular biology that use antibody-based staining methods. Immunohistochemistry or IHC staining of tissue sections (or immunocytochemistry, which...
independent tests. Tests commonly employed in tissue microarray include immunohistochemistry, and fluorescent in situ hybridization. Tissue microarrays are particularly...
The diagnosis of ICCB tumors is based on the histopathology and immunohistochemistry of their hematoxylin and eosin-stained tissues, particularly on identifying...
Methods commonly used to study protein structure and function include immunohistochemistry, site-directed mutagenesis, X-ray crystallography, nuclear magnetic...
The immunohistochemistry (IHC) test is a laboratory method that detects antibodies of prions (mis-shapen proteins thought to transmit bovine spongiform...
and immunohistochemistry. Because BSA is a small, stable, moderately non-reactive protein, it is often used as a blocker in immunohistochemistry. During...
Mammary secretory carcinoma (MSC), also termed secretory carcinoma of the breast, is a rare form of the breast cancers. MSC usually affects women but in...
molecular markers are characteristic of particular cancer types. Immunohistochemistry is also widely used in basic research to understand the distribution...
where nuclei are enlarged, the nucleus/cytoplasm ratio is elevated. Immunohistochemistry using Ki-67 stains the basal 1⁄3 to 1⁄2 of crypts, indicating a proliferative...
Angela Felicia; Tan, Sukmawati Tansil (2017). "The Use of BEREP4 Immunohistochemistry Staining for Detection of Basal Cell Carcinoma". Journal of Skin...
needs to be performed on biopsies, including immunofluorescence, immunohistochemistry, electron microscopy, flow cytometry, and molecular-pathologic analysis...
cells, B cells, and NK cells), megakaryocytes, and osteoclasts. In immunohistochemistry, CD31 is used primarily to demonstrate the presence of endothelial...
dendritic cells (FDCs), this becomes readily apparent only when immunohistochemistry is performed on frozen sections. In more conventional paraffin-embedded...
Pseudomyxoma peritonei (PMP) is a clinical condition caused by cancerous cells (mucinous adenocarcinoma) that produce abundant mucin or gelatinous ascites...
(2011). "Head and neck squamous cell carcinoma: mismatch repair immunohistochemistry and promoter hypermethylation of hMLH1 gene". Am J Otolaryngol. 32...
In cases where a metastasis from colorectal cancer is suspected, immunohistochemistry is used to ascertain correct diagnosis. Some proteins are more specifically...
antibodies associated with typhus. It can also be done with either immunohistochemistry (IHC) or polymerase chain reaction (PCR) tests excluding scrub typhus...
Burkitt lymphoma is a cancer of the lymphatic system, particularly B lymphocytes found in the germinal center. It is named after Denis Parsons Burkitt...
in the tissue (immunohistochemistry); or extraction of the protein and identification of its individual amino acids. Immunohistochemistry can identify AA...
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