Piece of DNA or RNA obtained by amplification chain reactions (PCR, LCR)
An amplicon sequence template that has been prepared for amplification. The target sequence to be amplified is colored green.
In molecular biology, an amplicon is a piece of DNA or RNA that is the source and/or product of amplification or replication events. It can be formed artificially, using various methods including polymerase chain reactions (PCR) or ligase chain reactions (LCR), or naturally through gene duplication. In this context, amplification refers to the production of one or more copies of a genetic fragment or target sequence, specifically the amplicon. As it refers to the product of an amplification reaction, amplicon is used interchangeably with common laboratory terms, such as "PCR product."
Artificial amplification is used in research,[1] forensics,[2] and medicine[1] for purposes that include detection and quantification of infectious agents,[3] identification of human remains,[4] and extracting genotypes from human hair.[2]
Natural gene duplication plays a major role in evolution. It is also implicated in several forms of human cancer including primary mediastinal B cell lymphoma and Hodgkin's lymphoma.[5] In this context the term amplicon can refer both to a section of chromosomal DNA that has been excised, amplified, and reinserted elsewhere in the genome, and to a fragment of extrachromosomal DNA known as a double minute, each of which can be composed of one or more genes. Amplification of the genes encoded by these amplicons generally increases transcription of those genes and ultimately the volume of associated proteins.[6]
^ abMeyers, Robert A., ed. (1995). Molecular Biology and Biotechnology: A Comprehensive Desk Reference. New York, NY: VCH Publishers. pp. 53, 585. ISBN 1-56081-925-1.
^ abWalsh, PS; Metzger, DA; Higuchi, R (1991). "Chelex 100 as a medium for simple extraction of DNA for PCR-based typing from forensic material". BioTechniques. 10 (4): 506–13. PMID 1867860.
^Gill, Peter; Ivanov, Pavel L.; Kimpton, Colin; Piercy, Romelle; Benson, Nicola; Tully, Gillian; Evett, Ian; Hagelberg, Erika; Sullivan, Kevin (1994). "Identification of the remains of the Romanov family by DNA analysis". Nature Genetics. 6 (2): 130–5. doi:10.1038/ng0294-130. PMID 8162066. S2CID 33557869.
^Rui, Lixin; Emre, N.C. Tolga; Kruhlak, Michael J.; Chung, Hye-Jung; Steidl, Christian; Slack, Graham; Wright, George W.; Lenz, Georg; et al. (2010). "Cooperative Epigenetic Modulation by Cancer Amplicon Genes". Cancer Cell. 18 (6): 590–605. doi:10.1016/j.ccr.2010.11.013. PMC 3049192. PMID 21156283.
^Bignell, G. R.; Santarius, T.; Pole, J. C.M.; Butler, A. P.; Perry, J.; Pleasance, E.; Greenman, C.; Menzies, A.; et al. (2007). "Architectures of somatic genomic rearrangement in human cancer amplicons at sequence-level resolution". Genome Research. 17 (9): 1296–303. doi:10.1101/gr.6522707. PMC 1950898. PMID 17675364.
In molecular biology, an amplicon is a piece of DNA or RNA that is the source and/or product of amplification or replication events. It can be formed artificially...
An amplicon sequence variant (ASV) is any one of the inferred single DNA sequences recovered from a high-throughput analysis of marker genes. Because these...
sequencing (sometimes called target amplicon sequencing) (TAS) is a next-generation DNA sequencing technique focusing on amplicons and specific genes. It is useful...
Found in the "yellow" amplicon. HSFY1, HSFY2 HSFX1, HSFX2 Found in the "blue" amplicon. PRY, PRY2 Found in the "blue" amplicon. Identified by similarity...
denaturation of the entire dsDNA amplicon. You make primers specific to the site you want to amplify. You "paint" the amplicon with a double-strand specific...
quantitative PCR-based technique initially designed to distinguish SNPs. The PCR amplicons are analyzed directly by temperature ramping and resulting liberation...
consists of multiple primer sets within a single PCR mixture to produce amplicons of varying sizes that are specific to different DNA sequences. By targeting...
Targeted amplicon sequencing relies on having some expectations about the composition of the community that is being studied. In target amplicon sequencing...
Common Name Marker M479 YCC Haplogroup R-M479 Nucleotide change C to T Amplicon size (bp) reference sequence 323 Polymorphism position from 5' end 107...
anticipated DNA target region (also sometimes referred to as the amplimer or amplicon), agarose gel electrophoresis may be employed for size separation of the...
sensitivity of CAPP-Seq is reported to be 2.5 molecules in 1,000,000. Tagged amplicon deep sequencing (TAM-Seq) allows targeted sequencing of entire genes to...
two minor genotypes of varicella-zoster virus strains: a practical two-amplicon approach used to genotype clinical isolates in Australia and New Zealand"...
template for subsequent cycles), MALBAC utilizes special primers that allow amplicons to have complementary ends and therefore to loop, preventing DNA from...
untargeted metagenomics and metatranscriptomics does not need a genetic marker, amplicon sequencing does. It uses a gene that is highly conserved as a genetic marker...
provide very specific melting curves from the single-stranded (ss) probe-to-amplicon hybrid. Idaho Technology and Roche have done much to popularize this use...
differentiation of root-associated microbial communities, Nevertheless, amplicon-based community profiling may not provide the functional characteristics...
referred to as 27F and 1492R; however, for some applications shorter amplicons may be necessary, for example for 454 sequencing with titanium chemistry...
and complex genomes of any size. Finally, the GS Amplicon Variant Analyzer aligns reads from amplicon samples against a reference, identifying variants...
acid sequencing library construction method that stably cross-links cDNA amplicons within biological samples. Sequencing data is then generated through an...
Advantages over 16S rRNA amplicon sequencing are: not being limited to bacteria; strain-level classification where amplicon sequencing only gets the genus;...
Li B, Feng Q, Shen Z, Lee CH, et al. (8 June 2017). "Detection of DNA Amplicons of Polymerase Chain Reaction Using Litmus Test". Scientific Reports. 7...
Pyrosequencing of bisulfite treated DNA. This is the sequencing of an amplicon made by a normal forward primer but a biotinylated reverse primer to PCR...
Microsatellites can be analysed and verified by established PCR amplification and amplicon size determination, sometimes followed by Sanger DNA sequencing. In forensics...
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