In genetics, shotgun sequencing is a method used for sequencing random DNA strands. It is named by analogy with the rapidly expanding, quasi-random shot grouping of a shotgun.
The chain-termination method of DNA sequencing ("Sanger sequencing") can only be used for short DNA strands of 100 to 1000 base pairs. Due to this size limit, longer sequences are subdivided into smaller fragments that can be sequenced separately, and these sequences are assembled to give the overall sequence.
In shotgun sequencing,[1][2] DNA is broken up randomly into numerous small segments, which are sequenced using the chain termination method to obtain reads. Multiple overlapping reads for the target DNA are obtained by performing several rounds of this fragmentation and sequencing. Computer programs then use the overlapping ends of different reads to assemble them into a continuous sequence.[1]
Shotgun sequencing was one of the precursor technologies that was responsible for enabling whole genome sequencing.
^ abStaden, R. (1979). "A strategy of DNA sequencing employing computer programs". Nucleic Acids Research. 6 (7): 2601–2610. doi:10.1093/nar/6.7.2601. PMC 327874. PMID 461197.
^Anderson, Stephen (1981). "Shotgun DNA sequencing using cloned DNase I-generated fragments". Nucleic Acids Research. 9 (13): 3015–3027. doi:10.1093/nar/9.13.3015. PMC 327328. PMID 6269069.
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In genetics, shotgunsequencing is a method used for sequencing random DNA strands. It is named by analogy with the rapidly expanding, quasi-random shot...
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to be assembled to obtain complete gene or genome sequences. The shotgunsequencing technique (used by The Institute for Genomic Research (TIGR) to sequence...
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but this applies to 16S rRNA amplicon sequencing as well and is a fundamental problem. With shotgunsequencing, it can be resolved by having a high coverage...
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genome was released. This version combined sequences gained from shotgunsequencing with the BAC-based sequence assemblies, which has helped to fill in...
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