Figure 1: Application of bisulfite treatment in whole genome bisulfite sequencing to convert unmethylated cytosine, not 5-methylcytosine, to uracil. During amplification by polymerase chain reaction, uracil is converted to thymine.[1]
Whole genome bisulfite sequencing is a next-generation sequencing technology used to determine the DNA methylation status of single cytosines by treating the DNA with sodium bisulfite before high-throughput DNA sequencing. The DNA methylation status at various genes can reveal information regarding gene regulation and transcriptional activities.[1] This technique was developed in 2009 along with reduced representation bisulfite sequencing after bisulfite sequencing became the gold standard for DNA methylation analysis.[2][3]
Whole genome bisulfite sequencing measures single-cytosine methylation levels genome-wide and directly estimates the ratio of molecules methylated rather than enrichment levels. Currently, this technique has recognized and tested approximately 95% of all cytosines in known genomes.[4] With the improvement of library preparation methods and next-generation sequencing technology over the past decade, whole genome bisulfite sequencing has become an increasingly widespread and informative method for analyzing DNA methylation in epigenomic-wide studies.[5]
^ abKawakatsu, Taiji (2020), Vaschetto, Luis M. (ed.), "Whole-Genome Bisulfite Sequencing and Epigenetic Variation in Cereal Methylomes", Cereal Genomics: Methods and Protocols, Methods in Molecular Biology, vol. 2072, New York, NY: Springer US, pp. 119–128, doi:10.1007/978-1-4939-9865-4_10, ISBN 978-1-4939-9865-4, PMID 31541442, S2CID 202711452, retrieved 2021-11-14
^Stirzaker, Clare; Taberlay, Phillippa C.; Statham, Aaron L.; Clark, Susan J. (2014-02-01). "Mining cancer methylomes: prospects and challenges". Trends in Genetics. 30 (2): 75–84. doi:10.1016/j.tig.2013.11.004. ISSN 0168-9525. PMID 24368016.
^Olova, Nelly; Krueger, Felix; Andrews, Simon; Oxley, David; Berrens, Rebecca V.; Branco, Miguel R.; Reik, Wolf (2018-03-15). "Comparison of whole-genome bisulfite sequencing library preparation strategies identifies sources of biases affecting DNA methylation data". Genome Biology. 19 (1): 33. doi:10.1186/s13059-018-1408-2. ISSN 1474-760X. PMC 5856372. PMID 29544553.
^Lister, Ryan; Pelizzola, Mattia; Dowen, Robert H.; Hawkins, R. David; Hon, Gary; Tonti-Filippini, Julian; Nery, Joseph R.; Lee, Leonard; Ye, Zhen; Ngo, Que-Minh; Edsall, Lee (2009-10-14). "Human DNA methylomes at base resolution show widespread epigenomic differences". Nature. 462 (7271): 315–322. Bibcode:2009Natur.462..315L. doi:10.1038/nature08514. ISSN 1476-4687. PMC 2857523. PMID 19829295.
^Zhou, Li; Ng, Hong Kiat; Drautz-Moses, Daniela I.; Schuster, Stephan C.; Beck, Stephan; Kim, Changhoon; Chambers, John Campbell; Loh, Marie (2019-07-17). "Systematic evaluation of library preparation methods and sequencing platforms for high-throughput whole genome bisulfite sequencing". Scientific Reports. 9 (1): 10383. Bibcode:2019NatSR...910383Z. doi:10.1038/s41598-019-46875-5. ISSN 2045-2322. PMC 6637168. PMID 31316107.
and 24 Related for: Whole genome bisulfite sequencing information
Wholegenomebisulfitesequencing is a next-generation sequencing technology used to determine the DNA methylation status of single cytosines by treating...
Bisulfitesequencing (also known as bisulphite sequencing) is the use of bisulfite treatment of DNA before routine sequencing to determine the pattern...
as methylated or unmethylated. Wholegenomebisulfitesequencing, also known as BS-Seq, which is a high-throughput genome-wide analysis of DNA methylation...
methylome of B cells along their differentiation cycle, using whole-genomebisulfitesequencing (WGBS), showed that there is a hypomethylation from the earliest...
loci, until wholegenomesequencing technologies became available. However, unlike traditional restriction based methods, bisulfitesequencing provided resolution...
least 50 million read pairs (100 million reads) per sample. Whole-GenomeBisulfiteSequencing (WGBS) to examine DNA methylation in cellular subpopulations...
normal cells using a wholegenome approach.[citation needed] Wholegenome or exome sequencing typically use high throughput DNA sequencing technologies. Limiting...
cell sequencing. Since 2013, methods have been created including whole-genome single-cell bisulfitesequencing to measure DNA methylation, whole-genome ChIP-sequencing...
needed] to generate a comprehensive genome-wide profiling of human DNA methylation. Similar to bisulfitesequencing and pyrosequencing, this method quantifies...
Illumina Infinium MethylationEPIC (850K) array) or with wholegenomebisulfitesequencing (WGBS) to collect DNA methylation data. In addition to collecting...
sites in genome that can be targets for the restriction enzyme; typically, a maximum of ~4100 landmarks can be assessed. Bisulfitesequencing-based methods...
time and will not be applicable for wholegenomesequencing approaches. However, the application of duplex sequencing for larger DNA targets will be more...
School for Boys, in Watford, Hertfordshire, United Kingdom Wholegenomebisulfitesequencing, a NGS technology to determine DNA methylation This disambiguation...
PCR. Bisulfitesequencing is another way to evaluate DNA methylation. Cytosine will be changed to uracil from being treated with sodium bisulfite, whereas...
been adapted and optimized for mRNA molecules, except for modified bisulfitesequencing for profiling 5-methylcytidine which was optimized for tRNAs and...
other sequencing methods combined with proteomic fractionations, and sequencing methods that find chemical modification of cytosines, like bisulfite sequencing...
a genome is present in a particular clone. The Human Genome Project found this application vital to mapping the cosmid clones they were sequencing, and...
to the difference in DNA sequence between the chromosomes. Whole-genomebisulfitesequencing (WGBS) is used to explore sequence-dependent allele-specific...
to amplify the target fragments. This method is deployed for DNA sequencing, genome walking, and DNA footprinting. A related technique is amplified fragment...
DNA (which can be a promoter) can be achieved through a method called bisulfite mapping. Methylated cytosine residues are unchanged by the treatment,...
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