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Primer walking is a technique used to clone a gene (e.g., disease gene) from its known closest markers (e.g., known gene). As a result, it is employed in cloning and sequencing efforts in plants, fungi, and mammals with minor alterations. This technique, also known as "directed sequencing," employs a series of Sanger sequencing reactions to either confirm the reference sequence of a known plasmid or PCR product based on the reference sequence (sequence confirmation service) or to discover the unknown sequence of a full plasmid or PCR product by designing primers to sequence overlapping sections (sequence discovery service).[1]
^Company, Azenta Life Sciences. "Primer Walking FAQ". web.genewiz.com. Retrieved 2021-11-20.
Primerwalking is a technique used to clone a gene (e.g., disease gene) from its known closest markers (e.g., known gene). As a result, it is employed...
Ferro-Luzzi Ames G (1993). "Single Specific Primer-Polymerase Chain Reaction (SSP-PCR) and Genome Walking". PCR Protocols. Methods in Molecular Biology...
"from the beginning". Gaps in the assembled sequence may be filled by primerwalking. The different strategies have different tradeoffs in speed and accuracy;...
major genes and genes underlying quantitative traits in plant species. Primerwalking Tanksley, Steven D.; Ganal, Martin W.; Martin, Gregory B. (February...
Ballet!". Pitchfork. February 8, 2010. Retrieved April 1, 2023. "A Chroma Primer". The Australian Ballet. March 27, 2014. "Winners of the 2007 Laurence Olivier...
is formed by the self-glucosylation of the glycogenin, forming a short primer chain. From the C-chain grows out B-chains, and from B-chains branch out...
attention. She said it would have been unprofessional to fix her dress while walking, so she continued with her walk and posture. Her fan club commended her...
Ahora by Ilia Calderón, veteran anchor of the network's daily newsmagazine Primer Impacto, who will make history as the first person of Afro-Latina heritage...
ambiguous, contigs of candidate sequence can be used as templates for primerwalking in primary infected tissue to generate the complete pathogen genome...
syndrome". EurekAlert!. Retrieved 2021-01-09. uoftmedgen (2020-04-17). "PrimerWalking to Personal Medicine: A shift in Medical Genomics". The MedGen Project...
"James Cameron's Story Of Science Fiction is a solid, albeit navel-gazing, primer". The A.V. Club. 30 April 2018. Archived from the original on 2 May 2018...
to Mayhem is the major label debut album by the American nu metal band Primer 55. Released on January 25, 2000, it is a reissue of the band's 1999 EP...
held in Lublin since 2005. Henio Zytomirski is one of the heroes of "The Primer" permanent exhibition at barrack 53 of the Majdanek Museum, an exhibition...
concentration. From a known sequence, a primer is designed to sequence across the circularized junction. This primer is used to jump 100 kb-300 kb intervals:...
obtaining the sequence desired for amplification from an already known primer site. While PCR needs information of already known sequences at both ends...
movement of the foot that occurs during foot landing while running or walking. Composed of three cardinal plane components: subtalar eversion, ankle...
material] Brian Eno, en vista de que David llevaba meses sin responder a su primer envío, cedió una de las piezas a Chris Martin, el cantante de Coldplay (Eno...
Simon & Schuster. ISBN 9780684838694. Edzard Ernst (15 January 2001). "A primer of complementary and alternative medicine commonly used by cancer patients...
Mark N. (2010). "Iran and Russia". In Wright, Robin B. (ed.). The Iran Primer: Power, Politics, and U.S. Policy. United States Institute of Peace. p. 186...
OCLC 1249433671. ISBN 978-3-931473-20-1 Miguel Ángel García-Villafuerte (2018). "Primer registro fósil de un lapsino (Araneae, Salticidae) en el ámbar de Chiapas...
Time-Life Books. pp. 106–07. Hugo O'Donnell, 7th Duke of Tetuan (2004). El primer Marqués de la Victoria, personaje silenciado en la reforma dieciochesca...
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