Pseudotyping is the process of producing viruses or viral vectors in combination with foreign viral envelope proteins. The result is a pseudotyped virus particle, also called a pseudovirus.[1] With this method, the foreign viral envelope proteins can be used to alter host tropism or increase or decrease the stability of the virus particles. Pseudotyped particles do not carry the genetic material to produce additional viral envelope proteins, so the phenotypic changes cannot be passed on to progeny viral particles. In some cases, the inability to produce viral envelope proteins renders the pseudovirus replication incompetent. In this way, the properties of dangerous viruses can be studied in a lower risk setting.[2]
Pseudotyping allows one to control the expression of envelope proteins. A frequently used protein is the glycoprotein G (VSV-G) from the Vesicular stomatitis virus (VSV) which mediates entry via the LDL receptor. Envelope proteins incorporated into the pseudovirus allow the virus to readily enter different cell types with the corresponding host receptor.
^Example for the development of pseudotype retroviral vectors in a work group of MHH Archived 2009-11-08 at the Wayback Machine
^Nie, Jianhui; Liu, Lin; Wang, Qing; Chen, Ruifeng; Ning, Tingting; Liu, Qiang; Huang, Weijin; Wang, Youchun (2019-02-19). "Nipah pseudovirus system enables evaluation of vaccines in vitro and in vivo using non-BSL-4 facilities". Emerging Microbes & Infections. 8 (1): 272–281. doi:10.1080/22221751.2019.1571871. ISSN 2222-1751. PMC 6455126. PMID 30866781.
Pseudotyping is the process of producing viruses or viral vectors in combination with foreign viral envelope proteins. The result is a pseudotyped virus...
Pseudovirus can refer to a virus artificially created by pseudotyping to contain envelope proteins from a different virus Pseudovirus (genus), a genus...
B, Rohr K, Kräusslich HG, Lehmann MJ (2009). "Visualizing fusion of pseudotyped HIV-1 particles in real time by live cell microscopy". Retrovirology...
J (August 2005). "Altering the tropism of lentiviral vectors through pseudotyping". Current Gene Therapy. 5 (4): 387–98. doi:10.2174/1566523054546224....
envelope proteins have been replaced as described are referred to as pseudotyped viruses. For example, the most popular retroviral vector for use in gene...
commonly, this is done using a lentivirus that encodes the transgene. Pseudotyped, self-inactivating lentiviruses are an effective method for the stable...
allow study of the later stages of the viral life cycle. RVPs formed by pseudotyping contain the native form of the viral Envelope (or Spike) protein, but...
diagnosis is based on the presence of the Albright hereditary osteodystrophy pseudotype but without the PTH resistance. Blood tests including calcium, phosphate...
specific affinity for VSV-G, a viral envelope glycoprotein often used to pseudotype retroviral and lentiviral vectors for gene therapy, allows it to be used...
and retroviral capsids. Such chimeras are termed pseudotyped viruses. Alphaviral envelope pseudotypes of retroviruses or lentiviruses are able to integrate...
where receptor is present on the cell. Conversely, using a surrogate or pseudotyped virus, is also a method of understanding cell tropism. In brief, these...
mildly immunogenic, lasting expression in non-dividing cells, capsid pseudotyping/engineering facilitates specific cell-targeting Lentivirus Up to 13.5...
Glycosaminoglycans, but This Binding Does Not Lead to Infection in a Pseudotype System". Journal of Virology. 78 (9): 4478–4486. doi:10.1128/JVI.78.9...
specific kind of cell. Viruses modified in this manner are said to be pseudotyped. Identification: Viral vectors are often given certain genes that help...
self-inactivating plasmids and creating a more universal tropism by pseudotyping a vesicular stomatitis virus glycoprotein. In chronic granulomatous disease...
inside the host cell but has not yet been activated by it. provirus pseudotyping Contents: A B C D E G H I K L M N O P Q R S T U V Z See also References...
immunodeficiency virus (HIV) type 1 can superinfect HIV-2-infected cells: pseudotype virions produced with expanded cellular host range". Proceedings of the...
K. Proc Natl Acad Sci U S A. 1994 Jan 18; 91(2):654-8. PMID 8290577 Pseudotyping of murine leukemia virus with the envelope glycoproteins of HIV generates...
can be limited to monosynaptic transmission to a neuron of origin by pseudotyping the G protein and putting the gene under Cre-control. This viral spread...
tropism assays using replication-competent virus and plasma-derived pseudotyped virions. He demonstrated the impact of low-dose ritonavir on the pharmacokinetics...
Characterization of the Bas-Congo virus glycoprotein and its function in pseudotyped viruses. J Virol. 2013;87(17):9558–9568. doi:10.1128/JVI.01183-13 Viralzone:...
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