A primer dimer (PD) is a potential by-product in the polymerase chain reaction (PCR), a common biotechnological method. As its name implies, a PD consists of two primer molecules that have attached (hybridized) to each other because of strings of complementary bases in the primers. As a result, the DNA polymerase amplifies the PD, leading to competition for PCR reagents, thus potentially inhibiting amplification of the DNA sequence targeted for PCR amplification. In quantitative PCR, PDs may interfere with accurate quantification.
A primerdimer (PD) is a potential by-product in the polymerase chain reaction (PCR), a common biotechnological method. As its name implies, a PD consists...
bands on PCR gels. Primerdimer formation often competes with formation of the DNA fragment of interest, and may be avoided using primers that are designed...
reaction (PCR) that reduces the presence of undesired products and primerdimers due to non-specific DNA amplification at room (or colder) temperatures...
that utilizes primers with a 3' extension block that can be removed by a thermostable RNase HII enzyme. This system reduces primer-dimers and allows for...
facilitates high specificity of the primers and reduces the production of nonspecific products, such as primerdimer. Also, use of a thermostable polymerase...
PCR (rhPCR) can reduce primer-dimer formation, and increase the number of assays in multiplex PCR. The method utilizes primers with a cleavable block...
using DNA binding dyes such as SYBR Green since the elimination of primer-dimers can be achieved through a simple change in the melting temperature....
which uses only forward primers, while the second reaction uses only reverse primers. This avoids the formation of primerdimer formation.[page needed]...
John Primer (born March 5, 1945, Camden, Mississippi, United States) is an American Chicago blues and electric blues singer and guitarist who played behind...
PCR artifacts (e.g. primer-dimers or truncated products) can increase the background noise and obscure the signal. Primer-dimers can also inhibit the...
news. 2020-09-09. "HostDime obtiene el primer certificado ISO 27701 emitido en Brasil". datacenterdynamics.com. 2020-12-15. "HostDime begins work on Colombian...
PCR reaction. NetPrimer also analyzes the thermodynamically important secondary structures such as hairpins, self and cross dimers, runs and repeats...
balance, resulting in inefficient ligation and an excess amount of primerdimers, respectively. Therefore, it is important to keep the molar concentration...
asymmetric dimer with DNA polymerase epsilon.) This polymerase synthesizes leading strand DNA in eukaryotes. (Thought to form an asymmetric dimer with DNA...
template strand. To begin synthesis, a short fragment of RNA, called a primer, must be created and paired with the template DNA strand. DNA polymerase...
The Oxford Chemistry Primers are a series of short texts providing accounts of a range of essential topics in chemistry and chemical engineering written...
originally linked the two nucleotides of the dimer. Subsequent steps in the repair process involve removal of the dimer remnants and repair synthesis to fill...
replication, where it is then processed by RNaseH to produce the 3' OH primer that DNA polymerase I uses to initiate leading strand DNA synthesis. RNA...
stem and the 3D domains (VPg domain) contacting the lower stem. The 3C dimer opens the RNA stem by forming a more stable interaction with single strands...
structure of DnaB is really a trimer of dimers. Both Ile119 and Ile125 are buried in the N-terminal domain dimer interface of DnaB and mutation of these...
such as I2, phenols, trimethyltin chloride, metalloporphyrins, and the dimer Rh2Cl2(CO)4. The donor properties are discussed in the ECW model. The relative...
deserts, are counties that lack maternity care resources. The March of Dimes defines a maternity care desert as a county that has no hospitals or birth...
pathways in which dimer or other oligomers are the reactive species have also been discovered, and for lithium amides such as LDA, dimer-based reactions...
Findings may include low platelets, low fibrinogen, high INR, or high D-dimer. Treatment is mainly directed towards the underlying condition. Other measures...
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