Global InformationNetprimer information
| Developer(s) | Premier Biosoft |
|---|---|
| Stable release | 1.10[citation needed]
/ 6 May 2002[citation needed] |
| Platform | Java applet[1] |
| Type | Bioinformatics |
| License | commercial |
| Website | http://www.premierbiosoft.com/netprimer/ |
NetPrimer is a gratis web-based tool used for analysing primers used in PCR to amplify a DNA sequence.[2] The software predicts the melting temperature of the primers using the nearest neighbor thermodynamic algorithm. The accurate prediction of the melting temperature (Tm) is one of the most important factors that governs the success of a PCR reaction.[3]
NetPrimer also analyzes the thermodynamically important secondary structures such as hairpins, self and cross dimers, runs and repeats.[4] These structures significantly affect the primer efficiency and therefore the success[clarification needed] of a PCR reaction.
NetPrimer can be used to determine the best primer pairs for a given set of experimental conditions. The program assigns a rating to each primer analyzed.[5] The rating is based on the proximity of the thermodynamic parameters to their ideal scores.
In addition to the primer quality, its molecular weight and optical activity (both in nmol/A260 and μg/A260) are also presented for quantitation. Primers are analyzed for their GC% (Guanine-Cytosine content).[6] This important parameter determines their annealing strength.
- ^ http://www.premierbiosoft.com/netprimer/netprlaunch/Help/xnetprlaunch.html (although it says "requires Java plug-in 1.4 or later for Windows", further down the page it also shows they've tested it on Mac OS X)
- ^ "Archived copy" (PDF). Archived from the original (PDF) on 2009-12-14. Retrieved 2009-01-07.
{{cite web}}: CS1 maint: archived copy as title (link) - ^ Dieffenbach, C.W.; Dveksler, G.S. (2003). PCR Primer: A Laboratory Manual, Second Edition (2003) Paperback. Cold Spring Harbor Laboratory Press. p. 61. ISBN 9780879696542. Retrieved 2015-05-17.
- ^ pga.mgh.harvard.edu/primerbank/help.html
- ^ "Archived copy" (PDF). Archived from the original (PDF) on 2009-01-10. Retrieved 2009-01-07.
{{cite web}}: CS1 maint: archived copy as title (link) - ^ Callahan, Johnny D.; Wu, Shuenn-Jue L.; Dion-Schultz, Amanda; Mangold, Beverly E.; Peruski, Leonard F.; Watts, Douglas M.; Porter, Kevin R.; Murphy, Gerald R.; Suharyono, Wuryadi; King, Chwan-Chuen; Hayes, Curtis G.; Temenak, Joseph J. (2001). "Development and Evaluation of Serotype- and Group-Specific Fluorogenic Reverse Transcriptase PCR (Taq Man) Assays for Dengue Virus" (PDF). Journal of Clinical Microbiology. 39 (11): 4119–4124. doi:10.1128/JCM.39.11.4119-4124.2001. PMC 88496. PMID 11682539.