Thermostable form of DNA polymerase I used in polymerase chain reaction
DNA polymerase I, thermostable
Large (Klenow) fragment of Taq polA, containing the polA and vestigial domains
Identifiers
Organism
Thermus aquaticus
Symbol
polA
UniProt
P19821
Search for
Structures
Swiss-model
Domains
InterPro
Taq polymerase is a thermostable DNA polymerase I named after the thermophilic eubacterial microorganism Thermus aquaticus, from which it was originally isolated by Chien et al. in 1976.[1] Its name is often abbreviated to Taq or Taq pol. It is frequently used in the polymerase chain reaction (PCR), a method for greatly amplifying the quantity of short segments of DNA.
T. aquaticus is a bacterium that lives in hot springs and hydrothermal vents, and Taq polymerase was identified[1] as an enzyme able to withstand the protein-denaturing conditions (high temperature) required during PCR.[2] Therefore, it replaced the DNA polymerase from E. coli originally used in PCR.[3]
^ abChien A, Edgar DB, Trela JM (September 1976). "Deoxyribonucleic acid polymerase from the extreme thermophile Thermus aquaticus". Journal of Bacteriology. 127 (3): 1550–7. doi:10.1128/jb.127.3.1550-1557.1976. PMC 232952. PMID 8432.
^Saiki RK, Gelfand DH, Stoffel S, Scharf SJ, Higuchi R, Horn GT, et al. (January 1988). "Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase". Science. 239 (4839): 487–91. Bibcode:1988Sci...239..487S. doi:10.1126/science.2448875. PMID 2448875.
^Saiki RK, Scharf S, Faloona F, Mullis KB, Horn GT, Erlich HA, Arnheim N (December 1985). "Enzymatic amplification of beta-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia". Science. 230 (4732): 1350–4. Bibcode:1985Sci...230.1350S. doi:10.1126/science.2999980. PMID 2999980. Archived from the original on 2008-12-19.
Taqpolymerase is a thermostable DNA polymerase I named after the thermophilic eubacterial microorganism Thermus aquaticus, from which it was originally...
heat-stable DNA polymerase, such as Taqpolymerase, an enzyme originally isolated from the thermophilic bacterium Thermus aquaticus. If the polymerase used was...
such as inactivating or inhibiting the binding of Taqpolymerase and late addition of Taqpolymerase, to increase product yield as well as provide a higher...
and nature. Taqpolymerase is a heat-stable enzyme of this family that lacks proofreading ability. DNA polymerase II is a family B polymerase encoded by...
the TaqMan probe significantly increases the specificity of the detection. TaqMan probes were named after the videogame Pac-Man (TaqPolymerase + PacMan...
replication. A DNA polymerase from the thermophilic bacterium, Thermus aquaticus (Taq) (PDB 1BGX, EC 2.7.7.7) is used in the polymerase chain reaction, an...
and proofreading properties compared with Taq DNA polymerase. Unlike Taq DNA polymerase, Pfu DNA polymerase possesses 3' to 5' exonuclease proofreading...
December 22, 1989, the journal Science awarded TaqPolymerase (and PCR) its first "Molecule of the Year". The 'Taq PCR' paper became for several years the most...
heat-resistant enzyme Taq DNA polymerase, one of the most important enzymes in molecular biology because of its use in the polymerase chain reaction (PCR)...
Taq or TAQ may refer to: Taq, Iran, a village in Semnan Province, Iran Taqpolymerase, a heat-stable enzyme used in polymerase chain reaction, or Thermus...
one-step RT-PCR kit with a mix of reverse transcriptase, Taq DNA polymerase, and a proofreading polymerase is selected and all necessary materials and equipment...
cloned into specific vectors without the requirement for DNA ligases. Taqpolymerase has a nontemplate-dependent terminal transferase activity that adds...
first protocols of polymerase chain reaction (PCR) amplification until Thermus aquaticus, the source of a heat-tolerant TaqPolymerase I, was discovered...
produce an alternative DNA polymerase to the commonly used Taqpolymerase. The T. litoralis polymerase, dubbed the vent polymerase, has been shown to have...
fidelity amplification compared with Taq. Magnesium is required as a co-factor for thermostable DNA polymerase. Taqpolymerase is a magnesium-dependent enzyme...
complementary 3' thymine overhangs. The insert is created by PCR using Taqpolymerase. This polymerase lacks 3' to 5' proofreading activity and, with a high probability...
Saiki started to use Thermophilus aquaticus (Taq) DNA polymerase to amplify segments of DNA. The Taqpolymerase was heat resistant and needed to be added...
segments of DNA in the polymerase chain reaction (PCR) process, before being replaced by thermostable enzymes such as Taqpolymerase. Just as the 5' → 3'...
Some of these enzymes are used in molecular biology, for example the Taqpolymerase used in PCR. "Thermophile" is derived from the Greek: θερμότητα (thermotita)...
various biotechnical processes. A good example of this would be how TaqPolymerase was isolated from the bacteria Thermus aquaticus and was then used to...
ligase, to join probe molecules together, and a thermostable polymerase (e.g., Taqpolymerase) to amplify those molecules involved in successful ligation...
In molecular biology, RNA polymerase (abbreviated RNAP or RNApol), or more specifically DNA-directed/dependent RNA polymerase (DdRP), is an enzyme that...
that complements the type having a dideoxynucleotide present. That the taqpolymerase used in PCR favors the ddGNTP, is a pattern observed in various research...
functional cloning. TOPO Cloning is a cloning method that uses Taqpolymerase. This is because Taq leaves a single adenosine overhang on the 3’ end of PCR reaction...
microbe scientists isolated the enzyme Taqpolymerase, which is now used in the powerful experimental technique, Polymerase chain reaction(PCR). Additionally...
processed through cycles of: (a) hybridization at 60 °C; (b) elongation via Taqpolymerase and a standard ligase; and (c) denaturation at 95 °C, forming progressively...
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