Global InformationSurveyor nuclease assay information
Surveyor nuclease assay is an enzyme mismatch cleavage assay used to detect single base mismatches or small insertions or deletions (indels).
Surveyor nuclease is part of a family of mismatch-specific endonucleases that were discovered in celery (CEL nucleases).[1] The enzyme recognizes all base substitutions and insertions/deletions, and cleaves the 3′ side of mismatched sites in both DNA strands with high specificity[2]
This assay has been used to identify and analyze mutations in a variety of organisms and cell types, as well as to confirm genome modifications following genome editing (using CRISPR/TALENs/zinc fingers).
- ^ Yang, B.; Wen, X.; Kodali, N. S.; Oleykowski, C. A.; Miller, C. G.; Kulinski, J.; Besack, D.; Yeung, J. A.; Kowalski, D. (2000-04-04). "Purification, cloning, and characterization of the CEL I nuclease". Biochemistry. 39 (13): 3533–3541. doi:10.1021/bi992376z. ISSN 0006-2960. PMID 10736152.
- ^ Qiu, Peter; Shandilya, Harini; D'Alessio, James M.; O'Connor, Kevin; Durocher, Jeffrey; Gerard, Gary F. (2004-04-01). "Mutation detection using Surveyor nuclease". BioTechniques. 36 (4): 702–707. doi:10.2144/04364PF01. ISSN 0736-6205. PMID 15088388.