Chromosome passenger complex (CPC) protein INCENP N terminal
Identifiers
Symbol
INCENP_N
Pfam
PF12178
InterPro
IPR022006
Available protein structures:
Pfam
structures / ECOD
PDB
RCSB PDB; PDBe; PDBj
PDBsum
structure summary
Inner centromere protein, ARK binding region
Identifiers
Symbol
INCENP_ARK-bind
Pfam
PF03941
InterPro
IPR005635
Available protein structures:
Pfam
structures / ECOD
PDB
RCSB PDB; PDBe; PDBj
PDBsum
structure summary
Inner centromere protein is a protein that in humans is encoded by the INCENP gene.[5][6][7] It is a regulatory protein in the chromosome passenger complex (CPC). It is involved in regulation of the catalytic proteins Aurora B and Aurora C.[8][9][10] It acts in association with two other proteins - Survivin and Borealin. These proteins form a tight three-helical bundle. The N-terminal domain of INCENP is the domain involved in formation of this three-helical bundle while its C-terminal domain is responsible for the interaction with Aurora B.[11][10]
In mammalian cells, two broad groups of centromere-interacting proteins have been described: constitutively binding centromere proteins and 'passenger' (or transiently interacting) proteins.[12] The constitutive proteins include CENPA (centromere protein A), CENPB, CENPC1, and CENPD.
The term 'passenger proteins' encompasses a broad collection of proteins that localize to the centromere during specific stages of the cell cycle.[13] These include CENPE; MCAK; KID; cytoplasmic dynein (e.g., DYNC1H1); CliPs (e.g. CLIP1); and CENPF/mitosin (CENPF). The inner centromere proteins (INCENPs),[5] the initial members of the passenger protein group, display a broad localization along chromosomes in the early stages of mitosis but gradually become concentrated at centromeres as the cell cycle progresses into mid-metaphase. During telophase, the proteins are located within the midbody in the intercellular bridge, where they are discarded after cytokinesis.[7][14]
^ abcGRCh38: Ensembl release 89: ENSG00000149503 – Ensembl, May 2017
^ abcGRCm38: Ensembl release 89: ENSMUSG00000024660 – Ensembl, May 2017
^"Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^"Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^ abEarnshaw WC, Cooke CA (Sep 1991). "Analysis of the distribution of the INCENPs throughout mitosis reveals the existence of a pathway of structural changes in the chromosomes during metaphase and early events in cleavage furrow formation". J Cell Sci. 98 (4): 443–61. doi:10.1242/jcs.98.4.443. PMID 1860899.
^Adams RR, Eckley DM, Vagnarelli P, Wheatley SP, Gerloff DL, Mackay AM, Svingen PA, Kaufmann SH, Earnshaw WC (Jul 2001). "Human INCENP colocalizes with the Aurora-B/AIRK2 kinase on chromosomes and is overexpressed in tumour cells". Chromosoma. 110 (2): 65–74. doi:10.1007/s004120100130. PMID 11453556. S2CID 13778759.
^ ab"Entrez Gene: INCENP inner centromere protein antigens 135/155kDa".
^Sasai, Kaori; Katayama, Hiroshi; Hawke, David H; Sen, Subrata (2016-01-01). "Aurora-C Interactions with Survivin and INCENP Reveal Shared and Distinct Features Compared with Aurora-B Chromosome Passenger Protein Complex". PLOS ONE. 11 (6): e0157305. Bibcode:2016PLoSO..1157305S. doi:10.1371/journal.pone.0157305. ISSN 1932-6203. PMC 4917241. PMID 27332895.
^Li, Xiangyu; Sakashita, Gyosuke; Matsuzaki, Hideki; Sugimoto, Kenji; Kimura, Keiji; Hanaoka, Fumio; Taniguchi, Hisaaki; Furukawa, Koichi; Urano, Takeshi (2004-11-01). "Direct association with inner centromere protein (INCENP) activates the novel chromosomal passenger protein, Aurora-C". The Journal of Biological Chemistry. 279 (45): 47201–47211. doi:10.1074/jbc.m403029200. ISSN 1083-351X. PMID 15316025.
^ abHonda, Reiko; Körner, Roman; Nigg, Erich A (2003-08-01). "Exploring the functional interactions between Aurora B, INCENP, and survivin in mitosis". Molecular Biology of the Cell. 14 (8): 3325–3341. doi:10.1091/mbc.e02-11-0769. ISSN 1939-4586. PMC 181570. PMID 12925766.
^Jeyaprakash, A. Arockia; Klein, Ulf R.; Lindner, Doris; Ebert, Judith; Nigg, Erich A.; Conti, Elena (2007-10-19). "Structure of a Survivin–Borealin–INCENP Core Complex Reveals How Chromosomal Passengers Travel Together". Cell. 131 (2): 271–285. doi:10.1016/j.cell.2007.07.045. ISSN 0092-8674. PMID 17956729. S2CID 15199822.
^Choo, K. H. Andy (1997). The centromere. Oxford [Oxfordshire]: Oxford University Press. ISBN 978-0-19-857780-5.
^Earnshaw WC, Mackay AM (September 1994). "Role of nonhistone proteins in the chromosomal events of mitosis". FASEB J. 8 (12): 947–56. doi:10.1096/fasebj.8.12.8088460. PMID 8088460. S2CID 14062246.
^Cutts SM, Fowler KJ, Kile BT, Hii LL, O'Dowd RA, Hudson DF, Saffery R, Kalitsis P, Earle E, Choo KH (July 1999). "Defective chromosome segregation, microtubule bundling and nuclear bridging in inner centromere protein gene (Incenp)-disrupted mice". Hum. Mol. Genet. 8 (7): 1145–55. doi:10.1093/hmg/8.7.1145. PMID 10369859.
Inner centromere protein is a protein that in humans is encoded by the INCENP gene. It is a regulatory protein in the chromosome passenger complex (CPC)...
inner centromere protein (INCENP) from the C-terminal to the conserved IN box domain, but Aurora B preferentially binds INCENP. The chromosomal passenger...
Borealin and INCENP. Each of the four components of the complex is required for the proper localization and function of the other three. INCENP stimulates...
in a dividing human cancer cell. DNA is stained blue, a protein called INCENP is green, and the microtubules are red. Each fluorophore is imaged separately...
includes the kinase protein Aurora B, its target and activating subunit INCENP and two other subunits, Survivin and Borealin/Dasra B (reviewed by Adams...
DL, et al. (November 2000). "Survivin and the inner centromere protein INCENP show similar cell-cycle localization and gene knockout phenotype". Current...
W (July 2005). "Recruitment of MKLP1 to the spindle midzone/midbody by INCENP is essential for midbody formation and completion of cytokinesis in human...
stability of the bipolar mitotic spindle. CDCA8 has been shown to interact with INCENP, Survivin and Aurora B kinase. GRCh38: Ensembl release 89: ENSG00000134690...
PT; Musacchio, A (Apr 29, 2005). "Mechanism of Aurora B activation by INCENP and inhibition by hesperadin". Molecular Cell. 18 (3): 379–91. doi:10.1016/j...
passenger complex (CPC) like Aurora B kinase, Survivin and INCENP. Direct phosphorylation of INCENP by Bub1 has been observed. RNAi mediated depletion of human...
kinetochore assembly. He discovered the "chromosomal passenger complex" of INCENP, Aurora B kinase, Survivin and Borealin – a major regulator of mitosis and...
K, Ranalli TA, Cowell JK (Jul 2006). "EVI5 protein associates with the INCENP-aurora B kinase-survivin chromosomal passenger complex and is involved in...
FJ, Vaux DL, et al. (2001). "Survivin and the inner centromere protein INCENP show similar cell-cycle localization and gene knockout phenotype". Curr...
W (July 2005). "Recruitment of MKLP1 to the spindle midzone/midbody by INCENP is essential for midbody formation and completion of cytokinesis in human...
Martineau-Thuillier S, Andreassen PR, Margolis RL (1999). "Colocalization of TD-60 and INCENP throughout G2 and mitosis: evidence for their possible interaction in signalling...
"Recapitulation of the Roberts syndrome cellular phenotype by inhibition of INCENP, ZWINT-1 and ZW10 genes". Gene. 331: 33–40. doi:10.1016/j.gene.2004.01.028...
"Recapitulation of the Roberts syndrome cellular phenotype by inhibition of INCENP, ZWINT-1 and ZW10 genes". Gene. 331: 33–40. doi:10.1016/j.gene.2004.01.028...
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