In the field of genetic sequencing, genotyping by sequencing, also called GBS, is a method to discover single nucleotide polymorphisms (SNP) in order to perform genotyping studies, such as genome-wide association studies (GWAS).[1] GBS uses restriction enzymes to reduce genome complexity and genotype multiple DNA samples.[2] After digestion, PCR is performed to increase fragments pool and then GBS libraries are sequenced using next generation sequencing technologies, usually resulting in about 100bp single-end reads.[3] It is relatively inexpensive and has been used in plant breeding.[2] Although GBS presents an approach similar to restriction-site-associated DNA sequencing (RAD-seq) method, they differ in some substantial ways.[4][5][6]
^Elshire, Robert J.; Glaubitz, Jeffrey C.; Sun, Qi; Poland, Jesse A.; Kawamoto, Ken; Buckler, Edward S.; Mitchell, Sharon E. (2011-05-04). "A Robust, Simple Genotyping-by-Sequencing (GBS) Approach for High Diversity Species". PLOS ONE. 6 (5): e19379. Bibcode:2011PLoSO...619379E. doi:10.1371/journal.pone.0019379. ISSN 1932-6203. PMC 3087801. PMID 21573248.
^Liu, Hui; Bayer, Micha; Druka, Arnis; Russell, Joanne R.; Hackett, Christine A.; Poland, Jesse; Ramsay, Luke; Hedley, Pete E.; Waugh, Robbie (2014-01-01). "An evaluation of genotyping by sequencing (GBS) to map the Breviaristatum-e (ari-e) locus in cultivated barley". BMC Genomics. 15: 104. doi:10.1186/1471-2164-15-104. ISSN 1471-2164. PMC 3922333. PMID 24498911.
^Davey, John W.; Hohenlohe, Paul A.; Etter, Paul D.; Boone, Jason Q.; Catchen, Julian M.; Blaxter, Mark L. (2011-07-01). "Genome-wide genetic marker discovery and genotyping using next-generation sequencing". Nature Reviews Genetics. 12 (7): 499–510. doi:10.1038/nrg3012. ISSN 1471-0056. PMID 21681211. S2CID 15080731.
^Campbell, Erin O.; Brunet, Byran M.T.; Dupuis, Julian R.; Sperling, Felix A.H. (2018). "Would an RRS by any other name sound as RAD?". Methods in Ecology and Evolution. 9 (9): 1920–1927. doi:10.1111/2041-210X.13038.
^Vaux, Felix; Dutoit, Ludovic; Fraser, Ceridwen I.; Waters, Jonathan M. (2022). "Genotyping-by-sequencing for biogeography". Journal of Biogeography. 50 (2): 262–281. doi:10.1111/jbi.14516.
and 28 Related for: Genotyping by sequencing information
sequencing, genotypingbysequencing, also called GBS, is a method to discover single nucleotide polymorphisms (SNP) in order to perform genotyping studies...
all genotyping is partial. That is, only a small fraction of an individual's genotype is determined, such as with (epi)GBS (Genotypingbysequencing) or...
them a viable option for sequencing small regions to perform SNP genotyping. Compared to other SNP genotyping methods, sequencing is in particular, suited...
and analyzing RAD tags are referred to as RAD markers. Although genotypingbysequencing presents an approach similar to the RAD-seq method, they differ...
chemical labeling of the sample. Nanopore sequencing has the potential to offer relatively low-cost genotyping, high mobility for testing, and rapid processing...
SNP genotyping (< 0.1% of the genome). The DNA sequencing methods used in the 1970s and 1980s were manual; for example, Maxam–Gilbert sequencing and Sanger...
Genotyping refers to the method used to determine an individual's genotype. There are a variety of techniques that can be used to assess genotype. The...
Sabah-based political parties coalition in Malaysia Gbesi language Genotypingbysequencing Gifu Broadcasting System, in Japan Goals breakdown structure, a...
of high throughput sequencing to genotype thousands of genetic markers across hundreds of individuals. This GenotypingbySequencing approach was widely...
addresses some of the present limitations of hybridization genotyping arrays. Although exome sequencing is more expensive than hybridization-based technologies...
D'Aquila RT (1 July 2000). "Comparison of Sequencingby Hybridization and Cycle Sequencing for Genotyping of Human Immunodeficiency Virus Type 1 Reverse...
markers.[clarification needed] Another area that is developing is genotypingbysequencing. To identify genes associated with traits, it is important to measure...
Human Genome Project, through its sequencing of the DNA, can help researchers understand diseases including: genotyping of specific viruses to direct appropriate...
"Classification and Characterization of Species within the Genus Lens Using Genotyping-by-Sequencing (GBS)". PLOS ONE. 10 (3): e0122025. Bibcode:2015PLoSO..1022025W...
Rai, H. S.; Rowe, C. A.; Richardson, B. A.; Mock, K. E. (2014). "Genotyping-by-Sequencing for Populus Population Genomics: An Assessment of Genome Sampling...
Hanna, GJ; et al. (July 2000). "Comparison of Sequencingby Hybridization and Cycle Sequencing for Genotyping of Human Immunodeficiency Virus Type 1 Reverse...
map for pearl millet, Pennisetum glaucum (L.) R. Br., using a genotyping-by-sequencing (GBS) approach" (PDF). Molecular Breeding. 35 (1). doi:10.1007/s11032-015-0212-x...
for harvesting and transporting corn. GBS (genotyping-by-sequencing) technology helps the corn industry by allowing for a better understanding of the...
markers appeared to support this, but more recent genome-wide Genotyping-by-Sequencing (GBS) data has shown that genetic diversity is actually quite high...
of genomics concerned with the sequencing, analysis and interpretation of the genome of an individual. The genotyping stage employs different techniques...
serving as the repository for the detailed information on samples, genotypes, raw sequencing reads and mapped reads, via a dedicated FTP site. "GIAB". Genome...
Development of Dasypyrum villosum Chromosome-Specific Markers via Genotyping-by-Sequencing (GBS)". Plant Disease. 103 (6). American Phytopathological Society:...
a Genetic Map for Onion (Allium cepa L.) Using Reference-Free Genotyping-by-Sequencing and SNP Assays". Frontiers in Plant Science. 8: 1606. doi:10.3389/fpls...
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