Sanger sequencing is a method of DNA sequencing that involves electrophoresis and is based on the random incorporation of chain-terminating dideoxynucleotides by DNA polymerase during in vitro DNA replication. After first being developed by Frederick Sanger and colleagues in 1977, it became the most widely used sequencing method for approximately 40 years. It was first commercialized by Applied Biosystems in 1986. More recently, higher volume Sanger sequencing has been replaced by next generation sequencing methods, especially for large-scale, automated genome analyses. However, the Sanger method remains in wide use for smaller-scale projects and for validation of deep sequencing results. It still has the advantage over short-read sequencing technologies (like Illumina) in that it can produce DNA sequence reads of > 500 nucleotides and maintains a very low error rate with accuracies around 99.99%.[1] Sanger sequencing is still actively being used in efforts for public health initiatives such as sequencing the spike protein from SARS-CoV-2[2] as well as for the surveillance of norovirus outbreaks through the Center for Disease Control and Prevention's (CDC) CaliciNet surveillance network.[3]
The Sanger (chain-termination) method for DNA sequencing.
^Shendure J, Ji H (October 2008). "Next-generation DNA sequencing". Nature Biotechnology. 26 (10): 1135–1145. doi:10.1038/nbt1486. PMID 18846087. S2CID 6384349.
^Daniels RS, Harvey R, Ermetal B, Xiang Z, Galiano M, Adams L, McCauley JW (November 2021). "A Sanger sequencing protocol for SARS-CoV-2 S-gene". Influenza and Other Respiratory Viruses. 15 (6): 707–710. doi:10.1111/irv.12892. PMC 8447197. PMID 34346163.
^Vega E, Barclay L, Gregoricus N, Williams K, Lee D, Vinjé J (August 2011). "Novel surveillance network for norovirus gastroenteritis outbreaks, United States". Emerging Infectious Diseases. 17 (8): 1389–1395. doi:10.3201/eid1708.101837. PMC 3381557. PMID 21801614.
Sangersequencing has been replaced by next generation sequencing methods, especially for large-scale, automated genome analyses. However, the Sanger...
comparative ease, the Sanger method was soon automated and was the method used in the first generation of DNA sequencers. Sangersequencing is the method which...
increasing share of the sequencing market. More genome data are now being produced by pyrosequencing than Sanger DNA sequencing. Pyrosequencing has enabled...
grouping of a shotgun. The chain-termination method of DNA sequencing ("Sangersequencing") can only be used for short DNA strands of 100 to 1000 base...
Frederick Sanger was born on 13 August 1918 in Rendcomb, a small village in Gloucestershire, England, the second son of Frederick Sanger, a general practitioner...
design is very different from that of Sangersequencing—also known as capillary sequencing or first-generation sequencing—which is based on electrophoretic...
Smith, was introduced by Applied Biosystems in 1987. It used the Sangersequencing method, a technology which formed the basis of the "first generation"...
offers several advantages over traditional sequencing methods such as Sangersequencing. Sangersequencing requires two reactions, one for the forward...
technique, also known as "directed sequencing," employs a series of Sangersequencing reactions to either confirm the reference sequence of a known plasmid...
chain-elongating inhibitors of DNA polymerase, used in the Sanger method for DNA sequencing. They are also known as 2',3' because both the 2' and 3' positions...
acid sequence of insulin, Frederick Sanger and his colleagues played a key role in the development of DNA sequencing techniques that enabled the establishment...
cloning and sequencing of the suspect gene. The amino acid sequence encoded by the gene is then predicted. NA Sequencing: Sangersequencing or Next-Generation...
methods to conduct DNA sequencing, Whole Exome Sequencing (WES) and Whole Genome Sequencing (WGS). Formal way of sequencing, the sanger technique had some...
The Wellcome Sanger Institute, previously known as The Sanger Centre and Wellcome Trust Sanger Institute, is a non-profit British genomics and genetics...
etc. There are many Next Generation Sequencingsequencing platforms available, postdating classical Sangersequencing methodologies. Other platforms include...
a number of large sequencing centers including those at Whitehead Institute, the Wellcome Sanger Institute (then called The Sanger Centre) based at the...
scientist. He is best known for his work on developing DNA sequencing technologies with Frederick Sanger and his contributions to the Caenorhabditis elegans...
genome). The DNA sequencing methods used in the 1970s and 1980s were manual; for example, Maxam–Gilbert sequencing and Sangersequencing. Several whole...
advancing the study of genetics, and replacing older methods such as Sangersequencing. This technology is starting to become more common in healthcare and...
third-generation sequencing was based in the headquarters in Constance, Germany. The fully automated NGS laboratories were certified under ISO 17025. The Sanger sequencing...
Biosystems SOLiD system. These techniques for sequencing DNA generate shorter fragments than Sangersequencing; Ion Torrent PGM System and 454 pyrosequencing...
produce a large amount of data at a fraction of the cost involved in Sangersequencing. TAS is also useful in DNA studies because it allows for amplification...
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