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Fetal bovine serum
Fetal bovine serum (FBS) is derived from the blood drawn from a bovine fetus via a closed system of collection at the slaughterhouse. Fetal bovine serum is the most widely used serum-supplement for the in vitro cell culture of eukaryotic cells. This is due to it having a very low level of antibodies and containing more growth factors, allowing for versatility in many different cell culture applications.
The globular protein, bovine serum albumin (BSA), is a major component of fetal bovine serum. The rich variety of proteins in fetal bovine serum maintains cultured cells in a medium in which they can survive, grow, and divide.
Because it is a biological product, FBS is not a fully defined media component, and as such varies in composition between batches.[1] As a result of this and in an attempt to minimize the possibility of transfer of adventitious agents, serum-free and chemically defined media (CDM) have been developed. However, the effectiveness of serum-free media is limited as many cell lines still require serum in order to grow, and many serum-free media formulations can only support the growth of narrowly-defined types of cells.[2]
^Dirks WG (2021). "Ethical Challenges Using Human Tumor Cell Lines in Cancer Research". Ethical Challenges in Cancer Diagnosis and Therapy. Recent Results in Cancer Research. Vol. 218. pp. 39–46. doi:10.1007/978-3-030-63749-1_4. ISBN 978-3-030-63748-4. PMID 34019161. S2CID 235072805.
^Yao T, Asayama Y (April 2017). "Animal-cell culture media: History, characteristics, and current issues". Reproductive Medicine and Biology. 16 (2): 99–117. doi:10.1002/rmb2.12024. PMC 5661806. PMID 29259457.
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Bovineserum albumin (BSA or "Fraction V") is a serum albumin protein derived from cows. It is often used as a protein concentration standard in lab experiments...
animal-derived components and cannot contain either fetalbovineserum, bovineserum or human serum. To achieve this, chemically defined media is commonly...
Human platelet lysate (or hPL) is a substitute supplement for fetalbovineserum (FBS) in experimental and clinical cell culture. It is a turbid, light-yellow...
it is commonly supplemented with 10% fetalbovineserum. Properly supplemented with serum or an adequate serum replacement, RPMI 1640 allows the cultivation...
may include saline solution, phosphate-buffered saline (PBS), or fetalbovineserum (FBS). VTM must be sterile to avoid introducing contamination to the...
fetalbovineserum. In the cryogenic freezing of heteroploid cell lines (MDCK, VERO, etc.) a mixture of 10% DMSO with 90% EMEM (70% EMEM + 30% fetal bovine...
embryonic stem (ES) and induced pluripotent stem (iPS) cells than fetalbovineserum, previously the standard for nutritive supplementation of ES and iPS...
the name "fetuin" (from Latin, fetus). Fetalbovineserum contains more fetuin than albumin, while adult serum contains more albumin than fetuin. Human...
animal tissue in culture involves the use of fetalbovineserum (FBS). FBS is a blood product extracted from fetal calves. This product supplies cells with...
(1975). "Bactericidal and hemolytic activity of complement in bovine colostrum and serum: effect of proteolytic enzymes and ethylene glycol tetraacetic...
often derived from the serum of animal blood, such as fetalbovineserum (FBS), bovine calf serum, equine serum, and porcine serum. One complication of...
serum creatinine, which is related to kidney failure, were low in mothers with high levels of male fetal cells. In contrast, women without male fetal...
hydrolysed in Dulbecco's modified Eagle's medium (DMEM) supplemented with fetalbovineserum (FBS) (a solution mimicking body fluid). After 30 days of submersion...
hours. The base medium for this cell line is RPMI-1640 Medium with a fetalbovineserum in a final concentration of 10%. 4T1 cells should not be allowed to...
culturing cells and tissue engineering, this crystal is found in fetalbovineserum, and is an important aspect of the extracellular matrix for culturing...
attenuated rubella virus WI-38 human embryonic cell line MEM (solution containing buffered salts, fetalbovineserum, human serum albumin and neomycin, etc.)...
achieve muscle differentiation in cultured meat without the use of fetalbovineserum (FBS) and without genetically modifying the cells. Mosa Meat is focused...
endometrial cells in monolayer culture: Dependence on a factor in fetalbovineserum". Journal of Cellular Biochemistry. 57 (2): 262–270. doi:10.1002/jcb...
in Dulbecco's modified Eagle's medium (DMEM) supplemented with 15% fetalbovineserum (FBS) in a humid, 5% CO2-enriched atmosphere at 28 °C. Under these...
emerged in recent years[when?], including the availability of GMP grade fetalbovineserum[citation needed]. The upstream part of a bioprocess refers to the...
cells are also used to study viral infections. High-glucose DMEM FBS fetalbovineserum: 5% (15% when freeze) GLU glutamine: 1% PSA regular antibiotics: 1%...
Schools in the West Bank Failed back syndrome Fasting blood sugar Fetalbovineserum Frontiers in Bioscience, an academic journal Function-Behaviour-Structure...
continually grow in culture medium like RPMI-1640 (with antibiotics and fetalbovineserum) and produce antibodies. Multiwell plates are used initially to grow...
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