Substitute supplement for fetal bovine serum (FBS)
Human platelet lysate (or hPL) is a substitute supplement for fetal bovine serum (FBS) in experimental and clinical cell culture. It is a turbid, light-yellow liquid that is obtained from human blood platelets after freeze/thaw cycle(s). The freeze/thaw cycle causes the platelets to lyse, releasing a large quantity of growth factors necessary for cell expansion. hPL has the highest concentration of growth factors of any serum suppliments.[1]
FBS-free cell culture media, e.g. with platelet lysate or chemically defined/ animal component free, are used for cell therapy or regenerative medicine. They are commercially available in GMP (good manufacturing practice)-quality which is generally basis for regulatory approval.[2]
^Doucet, C. et al. Platelet lysates promote mesenchymal stem cell expansion: A safety substitute for animal serum in cell-based therapy applications. Journal of Cellular Physiology 205, 228–236 (2005).
^Laner‑Plamberger et al. J Transl Med (2015) 13:354
Human plateletlysate (or hPL) is a substitute supplement for fetal bovine serum (FBS) in experimental and clinical cell culture. It is a turbid, light-yellow...
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Re-implantation of Culture-Expanded Mesenchymal Stem Cells using Autologous PlateletLysate Technique". Current Stem Cell Research & Therapy. 5 (1): 81–93. doi:10...
re-implantation of culture-expanded mesenchymal stem cells using autologous plateletlysate technique". Current Stem Cell Research & Therapy. 5 (1): 81–93. doi:10...
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organ (e.g. a segment of an intestine). tissue (e.g. limulus lysate) cell (e.g. platelets) Ligand binding assay when a ligand (usually a small molecule)...
basis of detection of bacterial endotoxin through the Limulus amebocyte lysate test for parenteral medications. Coagulogen contains a single 175-residue...
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size of the protein in addition to its identity. A sample (often cellular lysate) is separated on a polyacrylamide gel, transferred to a membrane and then...
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