This article is missing information about other gels with other molecules. Please expand the article to include this information. Further details may exist on the talk page.(January 2022)
In molecular biology, gel extraction or gel isolation is a technique used to isolate a desired fragment of intact DNA from an agarose gel following agarose gel electrophoresis. After extraction, fragments of interest can be mixed, precipitated, and enzymatically ligated together in several simple steps. This process, usually performed on plasmids, is the basis for rudimentary genetic engineering.
After DNA samples are run on an agarose gel, extraction involves four basic steps: identifying the fragments of interest, isolating the corresponding bands, isolating the DNA from those bands, and removing the accompanying salts and stain.
To begin, UV light is shone on the gel in order to illuminate all the ethidium bromide-stained DNA. Care must be taken to avoid exposing the DNA to mutagenic radiation for longer than absolutely necessary. The desired band is identified and physically removed with a cover slip or razor blade. The removed slice of gel should contain the desired DNA inside. An alternative method, utilizing SYBR Safe DNA gel stain and blue-light illumination, avoids the DNA damage associated with ethidium bromide and UV light.[1]
Several strategies for isolating and cleaning the DNA fragment of interest exist.
^Quest: An Invitrogen Publication for Discovery Vol. 4, Issue 2, pp. 44–45 (2007).
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bit larger than ethidium bromide, it does not bind to the column of a gelextraction as easily, making it less efficient when trying to clone a DNA fragment...
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chromatography (or gel filtration), affinity chromatography Protein extraction and solubilization Concentrating protein solutions Gel electrophoresis Gel electrophoresis...
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