Method for separation and analysis of biomolecules using agarose gel
Digital image of 3 plasmid restriction digests run on a 1% w/v agarose gel, 3 volt/cm, stained with ethidium bromide. The DNA size marker is a commercial 1 kbp ladder. The position of the wells and direction of DNA migration is noted.
Agarose gel electrophoresis is a method of gel electrophoresis used in biochemistry, molecular biology, genetics, and clinical chemistry to separate a mixed population of macromolecules such as DNA or proteins in a matrix of agarose, one of the two main components of agar. The proteins may be separated by charge and/or size (isoelectric focusing agarose electrophoresis is essentially size independent), and the DNA and RNA fragments by length.[1] Biomolecules are separated by applying an electric field to move the charged molecules through an agarose matrix, and the biomolecules are separated by size in the agarose gel matrix.[2]
Agarose gel is easy to cast, has relatively fewer charged groups, and is particularly suitable for separating DNA of size range most often encountered in laboratories, which accounts for the popularity of its use. The separated DNA may be viewed with stain, most commonly under UV light, and the DNA fragments can be extracted from the gel with relative ease. Most agarose gels used are between 0.7–2% dissolved in a suitable electrophoresis buffer.
^Kryndushkin DS, Alexandrov IM, Ter-Avanesyan MD, Kushnirov VV (December 2003). "Yeast [PSI+] prion aggregates are formed by small Sup35 polymers fragmented by Hsp104". The Journal of Biological Chemistry. 278 (49): 49636–43. doi:10.1074/jbc.M307996200. PMID 14507919.
^Sambrook J, Russel DW (2001). Molecular Cloning: A Laboratory Manual 3rd Ed. Cold Spring Harbor Laboratory Press. Cold Spring Harbor, NY.
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them to behave as long rods again. Gelelectrophoresis of large DNA or RNA is usually done by agarosegelelectrophoresis. See the "chain termination method"...
large molecules, especially DNA, by electrophoresis. Slabs of agarosegels (usually 0.7 - 2%) for electrophoresis are readily prepared by pouring the...
Temperature gradient gelelectrophoresis (TGGE) and denaturing gradient gelelectrophoresis (DGGE) are forms of electrophoresis which use either a temperature...
possible to separate them using standard agarosegelelectrophoresis. The limit of resolution depends on gel composition and field strength. and the mobility...
supporting medium, namely agarose or polyacrylamide. Variants of gelelectrophoresis include SDS-PAGE, free-flow electrophoresis, electrofocusing, isotachophoresis...
stain) in molecular biology laboratories for techniques such as agarosegelelectrophoresis. It is commonly abbreviated as EtBr, which is also an abbreviation...
technique of rapid agarosegelelectrophoresis are gel thickness, and the percentage of agarose within the gel. Boronate affinity electrophoresis utilizes boronic...
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either an acetate membrane soaked in a liquid buffer, or to a buffered agarosegel matrix, or into liquid in a capillary tube, and exposed to an electric...
chemical used to monitor the progress of agarosegelelectrophoresis and polyacrylamide gelelectrophoresis (PAGE) since DNA, RNA, and most proteins are...
biology, gel extraction or gel isolation is a technique used to isolate a desired fragment of intact DNA from an agarosegel following agarosegel electrophoresis...
a colour marker to monitor the process of agarosegelelectrophoresis and polyacrylamide gelelectrophoresis. Since bromophenol blue carries a slight negative...
(PCR), making it more suitable for analytical techniques such as agarosegelelectrophoresis, and chromatography. It is used in genetic fingerprinting, plasmid...
or tracking dye, to monitor the process of agarosegelelectrophoresis and polyacrylamide gelelectrophoresis. Bromophenol blue and orange G can also be...
through a matrix, usually an agarosegel, with the smaller molecules being pushed farther, faster. Capillary electrophoresis is a technique whereby small...
or RNA molecules. Gelelectrophoresis is a technique which separates molecules by their size using an agarose or polyacrylamide gel. This technique is...
digest are then separated by length through a process known as agarosegelelectrophoresis and transferred to a membrane via the Southern blot procedure...
DNA migration through the electrophoresisgel, which often resembles a comet. The comet assay (single-cell gelelectrophoresis) is a simple method for measuring...
hemoglobin from the red cells, it is introduced into a porous gel (usually made of agarose or cellulose acetate) and subjected to an electrical field, most...
amplified fragments are separated and visualized on denaturing on agarosegelelectrophoresis, either through autoradiography or fluorescence methodologies...
GelRed is an intercalating nucleic acid stain used in molecular genetics for agarosegel DNA electrophoresis. GelRed structurally consists of two ethidium...
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