Differential staining is a staining process which uses more than one chemical stain.[1] Using multiple stains can better differentiate between different microorganisms or structures/cellular components of a single organism.
Differential staining is used to detect abnormalities in the proportion of different white blood cells in the blood. The process or results are called a WBC differential. This test is useful because many diseases alter the proportion of certain white blood cells. By analyzing these differences in combination with a clinical exam and other lab tests, medical professionals can diagnose disease.
One commonly recognizable use of differential staining is the Gram stain. Gram staining uses two dyes: Crystal violet and Fuchsin or Safranin (the counterstain) to differentiate between Gram-positive bacteria (large Peptidoglycan layer on outer surface of cell) and Gram-negative bacteria.
Acid-fast stains are also differential stains.
^Krueger, Woodrow B. (1986). Laboratory procedures for general microbiology. Kendall/Hunt Pub. Co. p. 25. ISBN 9780840338044. Retrieved 21 December 2021.
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Differentialstaining is a staining process which uses more than one chemical stain. Using multiple stains can better differentiate between different microorganisms...
the presence of a specific compound. Staining and fluorescent tagging can serve similar purposes. Biological staining is also used to mark cells in flow...
stain using normal techniques such as simple staining and gram staining. Special techniques for endospore staining include the Schaeffer–Fulton stain...
automated differential counts: digital image processing of microscope slides and flow cytometry techniques using light scattering and cell staining. These...
Gram stain (Gram staining or Gram's method), is a method of staining used to classify bacterial species into two large groups: gram-positive bacteria...
Trichrome staining is a histological staining method that uses two or more acid dyes in conjunction with a polyacid. Staining differentiates tissues by...
C37H27N3Na2O9S3. It is used as a stain in histology, and stains collagen blue in tissue sections. It can be used in some differentialstaining techniques such as Mallory's...
ISBN 978-0816039494. Alexander, M. P. (1 January 1969). "DifferentialStaining of Aborted and Nonaborted Pollen". Stain Technology. 44 (3): 117–122. doi:10.3109/10520296909063335...
Acridine orange staining has to be performed at an acidic pH to obtain the differentialstaining, which allows bacterial cells to stain orange and tissue...
needed] Giemsa stain is also a differentialstain, such as when it is combined with Wright stain to form Wright-Giemsa stain. It can be used to study the...
Romanowsky staining is a prototypical staining technique that was the forerunner of several distinct but similar stains widely used in hematology (the...
connective tissue stains, such as Mallory's stain, Gömöri trichrome stain, and Carstair's Method. It is used in differentialstaining. "Stainsfile - Aniline...
Leishman stain, also known as Leishman's stain, is used in microscopy for staining blood smears. It is generally used to differentiate between and identify...
"eosinophilic" or "sudanophilic"). Alcian blue staining can be combined with H&E staining, PAS staining and van Gieson staining methods. Alcian blue can be used to...
phenol and basic fuchsin that is used in bacterial staining procedures. It is commonly used in the staining of mycobacteria because it has an affinity for...
Mucicarmine stain is a staining procedure used for different purposes. In microbiology the stain aids in the identification of a variety of microorganisms...
cell scattergrams using cytochemical staining techniques. Leonard Ornstein, who had helped to develop the staining system on the Rapid Cell Spectrophotometer...
the final staining of the tissue that can cause a variety of problems. It can be strong background staining, weak target antigen staining and presence...
In healthcare, a differential diagnosis (DDx) is a method of analysis that distinguishes a particular disease or condition from others that present with...
Ziehl–Neelsen stain (Z-N stain), the Kinyoun method of staining does not require heating. In the Ziehl–Neelsen stain, heat acts as a physical mordant while phenol...
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