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Water blue information


Water blue
Identifiers
CAS Number
  • 28631-66-5 checkY
3D model (JSmol)
  • Interactive image
ChemSpider
  • 21173243
ECHA InfoCard 100.044.634 Edit this at Wikidata
EC Number
  • 249-113-9
PubChem CID
  • 139030972
UNII
  • 44D829LK4D checkY
CompTox Dashboard (EPA)
  • DTXSID9044904 Edit this at Wikidata
InChI
  • InChI=1S/C32H27N3O9S3.2Na/c33-20-26-25(6-5-11-29(26)45(36,37)38)32(21-12-16-23(17-13-21)34-27-7-1-3-9-30(27)46(39,40)41)22-14-18-24(19-15-22)35-28-8-2-4-10-31(28)47(42,43)44;;/h1-19,34H,20,33H2,(H,36,37,38)(H,39,40,41)(H,42,43,44);;/q;2*+1/p-2
    Key: WJHQBPPQVMBLSW-UHFFFAOYSA-L
SMILES
  • [H+].c1ccc(c(c1)Nc2ccc(cc2)C(=C3C=CC(=Nc4ccccc4S(=O)(=O)[O-])C=C3)c5cccc(c5CN)S(=O)(=O)[O-])S(=O)(=O)[O-].[Na+].[Na+]
Properties
Chemical formula
C32H25N3O9S3Na2
Molar mass 737.72374 g/mol
Hazards
GHS labelling:
Pictograms
GHS07: Exclamation mark
Signal word
Warning
Hazard statements
H315, H317, H319, H335
Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa).
checkY verify (what is checkY☒N ?)
Infobox references

Water blue, also known as aniline blue, Acid blue 22, Soluble Blue 3M, Marine Blue V, or C.I. 42755, is a chemical compound used as a stain in histology. Water blue stains collagen blue in tissue sections. It is soluble in water and slightly soluble in ethanol.

Water blue is also available in mixture with methyl blue, under the names Aniline Blue WS, Aniline blue, China blue, or Soluble blue.[1][2]

It can be used in the Mallory's trichrome stain of connective tissue and Gömöri trichrome stain of muscle tissue. It is used in differential staining.[3]

  1. ^ Lillie, R. D. (1969). "H. J. Conn's Biological stains : a handbook on the nature and uses of the dyes employed in the biological laboratory". Trove (National Library of Australia). Retrieved February 8, 2019.
  2. ^ "Aniline Blue WS". StainsFile.
  3. ^ Nowicki, Marcin; et al. (15 May 2013), "A simple dual stain for detailed investigations of plant-fungal pathogen interactions", Vegetable Crops Research Bulletin, 77, Vegetable Crops Research bulleting, InHort & Versita: 61–74, doi:10.2478/v10032-012-0016-z

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