Technique used in bacteriology to identify the presence of endospores in a bacterial sample
Endospore stain on Bacillus subtilis. The spore is stained green and the vegetative cell is stained a pinkish red color.
Endospore staining is a technique used in bacteriology to identify the presence of endospores in a bacterial sample.[1] Within bacteria, endospores are protective structures used to survive extreme conditions, including high temperatures making them highly resistant to chemicals.[2] Endospores contain little or no ATP which indicates how dormant they can be. Endospores contain a tough outer coating made up of keratin which protects them from nucleic DNA as well as other adaptations. Endospores are able to regerminate into vegetative cells, which provides a protective nature that makes them difficult to stain using normal techniques such as simple staining and gram staining. Special techniques for endospore staining include the Schaeffer–Fulton stain and the Moeller stain.
^Microbiology An Introduction Tenth Edition; Tortora Funke Case
^Leboffe, Michael (2015). Microbiology Laboratory Theory and Application. Englewood, CO: Morton Publishing. pp. |page=215. ISBN 978-1-61731-250-2.
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simple staining and gram staining. Special techniques for endosporestaining include the Schaeffer–Fulton stain and the Moeller stain. Endospores were first...
the rest of the cell stains blue. Another staining technique for endospores is the Schaeffer-Fulton stain, which stainsendospores green and bacterial...
Differential staining can also be used to color different organelles within one organism which can be seen in endosporestaining. Gram staining is used to...
counterstain. Malachite green is also used in endosporestaining, since it can directly stainendospores within bacterial cells; here a safranin counterstain...
cell nuclei red. This is the classic counterstain in both Gram stains and endosporestaining. It can also be used for the detection of cartilage, mucin and...
Bacillus, B. subtilis is rod-shaped, and can form a tough, protective endospore, allowing it to tolerate extreme environmental conditions. B. subtilis...
Moeller staining involves the use of a steamed dye reagent in order to increase the stainability of endospores. Carbol fuchsin is the primary stain used...
and like other members of the genus Bacillus, can produce protective endospores. They have a wide range of virulence factors, including phospholipase...
Gram-positive staining common to class Bacilli. For example, E. coli is a rod-shaped bacterium that can be described as "a bacillus", but it stains Gram-negative...
structures called endospores. Endospores develop within the cytoplasm of the cell; generally, a single endospore develops in each cell. Each endospore contains...
crystal violet dye and stains purple in the Gram stain. Bacteria within the Deinococcota group may also exhibit gram-positive staining but contain some cell...
Clostridium are obligate anaerobe and capable of producing endospores. They generally stain gram-positive, but as well as Bacillus, are often described...
Bacillus can reduce themselves to oval endospores and can remain in this dormant state for years. The endospore of one species from Morocco is reported...
Rapid Modified Auramine O Fluorescent staining has specific binding to slowly-growing mycobacteria for yellow staining against a dark background. Newer methods...
responsible for the pathogenicity. It forms a protective layer called endospore by which it can remain inactive for many years and suddenly becomes infective...
surrounded by a thin delicate wall of unaltered cellulose called the endospore or intine, and a tough resistant outer cuticularized wall composed largely...
growing best under anaerobic conditions. Actinomyces species may form endospores, and while individual bacteria are rod-shaped, Actinomyces colonies form...
genus of rod-shaped bacteria that exhibits Gram-positive or Gram-variable staining that belongs in the family Bacillaceae within the order Bacillales. The...
tissue by methods of Papanicolaou or Grocott's methenamine silver staining. These stains can demonstrate spherules and surrounding inflammation.[citation...
pseudo-outer membrane that causes them to stain gram-negative. Many Bacillota (Firmicutes) produce endospores, which are resistant to desiccation and can...
sediment, and the rhizosphere of willow roots. All members can produce endospores and most are motile. Priestia species can grow in temperatures ranging...
Gram-positive, rod-shaped, and facultatively anaerobic, and do not produce endospores. The major human pathogen in the genus Listeria is L. monocytogenes. It...
of gram-positive, heterotrophic, rod-shaped bacteria that may produce endospores. Motile members of this family are characterized by peritrichous flagella...
binds to lipids, consequently staining and fixing them, making the lipids then visible during electron scanning. In the staining of plasma membrane like structures...
surfaces like floors and building premises.[citation needed] Bacterial endospores are most resistant to disinfectants, but some fungi, viruses and bacteria...
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