Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa).
Infobox references
Chemical compound
Carbol fuchsin, carbol-fuchsin, carbolfuchsin, or Castellani's paint (CAS 4197-24-4) is a mixture of phenol and basic fuchsin that is used in bacterial staining procedures. It is commonly used in the staining of mycobacteria because it has an affinity for the mycolic acids found in their cell membranes.
It is a component of Ziehl–Neelsen stain, a differential stain.[2][3]
Carbol fuchsin is used as the primary stain dye to detect acid-fast bacteria because it is more soluble in the cells' wall lipids than in the acid alcohol. If the bacteria is acid-fast the bacteria will retain the initial red color of the dye because they are able to resist the destaining by acid alcohol (0.4–1% HCl in 70% EtOH).[4] Additionally, it can be used for the staining of bacterial spores.
Carbol-fuchsin is also used as a topical antiseptic and antifungal.[citation needed]
^Angra P, Ridderhof J, Smithwick R (July 2003). "Comparison of two different strengths of carbol fuchsin in Ziehl-Neelsen staining for detecting acid-fast bacilli". J. Clin. Microbiol. 41 (7): 3459. doi:10.1128/JCM.41.7.3459.2003. PMC 165351. PMID 12843125.
^Selvakumar N, Rahman F, Rajasekaran S, et al. (August 2002). "Inefficiency of 0.3% carbol fuchsin in ziehl-neelsen staining for detecting acid-fast bacilli". J. Clin. Microbiol. 40 (8): 3041–3. doi:10.1128/JCM.40.8.3041-3043.2002. PMC 120628. PMID 12149374.
^Sokolovská, Ivana; Rozenberg, Raoul; Riez, Christophe; et al. (2003-12-01). "Carbon Source-Induced Modifications in the Mycolic Acid Content and Cell Wall Permeability of Rhodococcus erythropolis E1". Applied and Environmental Microbiology. 69 (12): 7019–7027. Bibcode:2003ApEnM..69.7019S. doi:10.1128/AEM.69.12.7019-7027.2003. ISSN 0099-2240. PMC 309960. PMID 14660344.
Carbolfuchsin, carbol-fuchsin, carbolfuchsin, or Castellani's paint (CAS 4197-24-4) is a mixture of phenol and basic fuchsin that is used in bacterial...
phenol (carbol of carbolfuchsin) acts as the chemical mordant. Since the Kinyoun stain is a cold method (no heat applied), the concentration of carbol fuschin...
counterstain. Through the use of malachite green and a diluted ratio of carbolfuchsin, fixing bacteria in osmic acid was a great way to ensure no blending...
decolorization with ethanol or acetone Counterstaining with safranin. Carbolfuchsin is sometimes substituted for safranin since it more intensely stains...
bacteriologist. He was a professor in Lübeck. Franz Ziehl introduced the carbolfuchsin stain for the tubercle bacillus in 1882. With pathologist Friedrich...
steamed dye reagent in order to increase the stainability of endospores. Carbolfuchsin is the primary stain used in this method. Endospores are stained red...
with phenol (also called carbolic acid) as an accentuator it is called carbolfuchsin and is used for the Ziehl–Neelsen and other similar acid-fast staining...
such as in Whitfield's ointment, Friar's Balsam, and Balsam of Peru Carbolfuchsin (Castellani's paint) Ciclopirox (ciclopirox olamine) – a hydroxypyridone...
method, rather than regular positive stains like methylene blue or carbolfuchsin, is that prior fixation by heat or alcohol is not needed, so the organisms...
be stained using simple stains such as methylene blue, safranin, and carbolfuchsin. These scientists, along with a few others, found out that spores were...
Gram staining, but Mycobacterium leprae was traditionally stained with carbolfuchsin in the Ziehl–Neelsen stain. Because the bacilli are less acid-fast than...
with hemolysin and eosin stains. The clubs could also be stained by carbolfuchsin with decolourisation by weak hydrochloric acid and counter-staining...
of Tropical Medicine. Castellani died in 1971. Castellani's paint (Carbolfuchsin solution) is still occasionally used to treat fungal skin infections...
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