Global Information Lookup Global Information

Coomassie brilliant blue information


Coomassie Brilliant Blue R-250
Skeletal formula of Coomassie brilliant blue R-250
Space-filling model of the coomassie brilliant blue R-250 molecule
Names
Other names
C.I. 42660, C.I. Acid Blue 83
Brilliant indocyanine 6B, Brillantindocyanin 6B
Brilliant Cyanine 6B, Serva Blue R
Identifiers
CAS Number
  • 6104-59-2 checkY
3D model (JSmol)
  • Interactive image
ECHA InfoCard 100.025.509 Edit this at Wikidata
PubChem CID
  • 61365
UNII
  • TKL1K0PT9A checkY
CompTox Dashboard (EPA)
  • DTXSID80910613 Edit this at Wikidata
SMILES
  • CCN(CC1=CC(=CC=C1)S(=O)(=O)O)C2=CC=C(C=C2)C(=C3C=CC(=[N+](CC)CC4=CC(=CC=C4)S(=O)(=O)[O-])C=C3)C5=CC=C(C=C5)NC6=CC=C(C=C6)OCC.[Na+]
Properties
Chemical formula
C45H44N3NaO7S2 (Sodium salt)
Molar mass 825.97 g/mol
Solubility in water
Insoluble in cold, slightly soluble in hot (bright red blue)
Solubility in ethanol Slightly soluble
Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa).
checkY verify (what is checkY☒N ?)
Infobox references
Coomassie brilliant blue G-250
Solid Coomassie brilliant blue G
Brilliant blue G in isopropanol solution
Names
Other names
C.I. 42655, C.I. Acid Blue 90
Brilliant indocyanine G, Brillantindocyanin G
Xylene Brilliant Cyanine G, Serva Blue G
Identifiers
CAS Number
  • 6104-58-1
3D model (JSmol)
  • Interactive image
ECHA InfoCard 100.025.509 Edit this at Wikidata
KEGG
  • D10799 checkY
PubChem CID
  • 6324599
CompTox Dashboard (EPA)
  • DTXSID80910613 Edit this at Wikidata
SMILES
  • CCN(CC1=CC(=CC=C1)S(=O)(=O)O)C2=CC(=C(C=C2)/C(=C\3/C=CC(=[N+](CC)CC4=CC(=CC=C4)S(=O)(=O)O)C=C3C)/C5=CC=C(C=C5)NC6=CC=C(C=C6)OCC)C
Properties
Chemical formula
C47H50N3NaO7S2 (Sodium salt)
Molar mass 856.03 g/mol
Solubility in water
Slightly soluble in cold, soluble in hot (bright blue)
Solubility in ethanol Soluble
Pharmacology
Legal status
  • CA: ℞-only[1]
Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa).
Infobox references

Coomassie brilliant blue is the name of two similar triphenylmethane dyes that were developed for use in the textile industry but are now commonly used for staining proteins in analytical biochemistry. Coomassie brilliant blue G-250 differs from Coomassie brilliant blue R-250 by the addition of two methyl groups. The name "Coomassie" is a registered trademark of Imperial Chemical Industries.

  1. ^ "Health product highlights 2021: Annexes of products approved in 2021". Health Canada. 3 August 2022. Retrieved 25 March 2024.

and 27 Related for: Coomassie brilliant blue information

Request time (Page generated in 0.8186 seconds.)

Coomassie brilliant blue

Last Update:

biochemistry. Coomassie brilliant blue G-250 differs from Coomassie brilliant blue R-250 by the addition of two methyl groups. The name "Coomassie" is a registered...

Word Count : 2571

Brilliant blue FCF

Last Update:

that brilliant blue FCF has a similar structure. This led them to test a related dye, brilliant blue G (also known as Coomassie brilliant blue) in rats...

Word Count : 2236

Brilliant blue

Last Update:

cresyl blue, a stain used in microscopy Coomassie brilliant blue, a dye Remazol Brilliant Blue R, a dye "Brilliant Blue", a side B song on the single "Kyū...

Word Count : 122

Coomassie

Last Update:

Coomassie may refer to: Coomassie brilliant blue, a dye Coomassie, an historical name for Kumasi, Ghana, and namesake of the dye Ibrahim Coomassie, Nigerian...

Word Count : 61

Bradford protein assay

Last Update:

absorbance shift of the dye Coomassie brilliant blue G-250. The Coomassie brilliant blue G-250 dye exists in three forms: anionic (blue), neutral (green), and...

Word Count : 3258

Polyacrylamide gel electrophoresis

Last Update:

electrophoresis, the gel may be stained (for proteins, most commonly with Coomassie brilliant blue R-250 or autoradiography; for nucleic acids, ethidium bromide;...

Word Count : 4717

Gel electrophoresis of proteins

Last Update:

of the protein. BN-PAGE is a native PAGE technique, where the Coomassie brilliant blue dye provides the necessary charges to the protein complexes for...

Word Count : 2351

Electrophoretic color marker

Last Update:

blue color that can be detected visually. Coomassie Brilliant Blue R-250 (red), is typically used for electrophoresis, while Coomassie Brilliant Blue...

Word Count : 1236

Capacitation

Last Update:

the occurrence of the acrosome reaction. A simple method uses Coomassie brilliant blue G250 to stain cells, providing visual evidence of intact or reacted...

Word Count : 2027

Spectrophotometry

Last Update:

compound. This coloring can be accomplished by either a dye such as Coomassie Brilliant Blue G-250 dye measured at 595 nm or by an enzymatic reaction as seen...

Word Count : 3649

Ouchterlony double immunodiffusion

Last Update:

sensitivity of the assay can be increased by using a stain such as Coomassie brilliant blue, this is done by repeated staining and destaining of the assay...

Word Count : 985

Molecular biology

Last Update:

properties of a dye called Coomassie Brilliant Blue G-250. Coomassie Blue undergoes a visible color shift from reddish-brown to bright blue upon binding to protein...

Word Count : 5527

Calibration curve

Last Update:

colorimetric assay that measures protein concentration. The reagent Coomassie brilliant blue turns blue when it binds to arginine and aromatic amino acids present...

Word Count : 1438

Crystal violet

Last Update:

several weeks. Crystal violet can be used as an alternative to Coomassie brilliant blue (CBB) in staining of proteins separated by SDS-PAGE, reportedly...

Word Count : 3334

Oligoclonal band

Last Update:

"SDS-PAGE (sodium dodecyl sulphate polyacrylamide gel electrophoresis)/Coomassie blue staining", and (b) the combination of isoelectric focusing/silver staining...

Word Count : 2556

Gel electrophoresis

Last Update:

ultraviolet light, while protein may be visualised using silver stain or Coomassie brilliant blue dye. Other methods may also be used to visualize the separation...

Word Count : 5057

Biotinylation

Last Update:

seen quickly and quantitatively for biotinylated proteins by Coomassie brilliant blue staining. Lectez, Benoît; Migotti, Rebekka; Lee, So Young; Ramirez...

Word Count : 2922

Proteome

Last Update:

separated by molecular weight using SDS-PAGE. The gel is stained with Coomassie brilliant blue or silver to visualize the proteins. Spots on the gel are proteins...

Word Count : 2993

Electroblotting

Last Update:

being used. Typically the electrophoresis gel is stained with Coomassie brilliant blue following the transfer to ensure that a sufficient quantity of...

Word Count : 395

Silver staining

Last Update:

macromolecules that have been separated in a variety of supports. Classical Coomassie brilliant blue staining can usually detect a 50 ng protein band; silver staining...

Word Count : 1500

CBB

Last Update:

components Connexion by Boeing, a mobile ISP formerly offered by Boeing Coomassie brilliant blue, a dye commonly used to stain proteins in certain gels Internet...

Word Count : 258

Protein methods

Last Update:

Most commonly, Coomassie brilliant blue G-250 dye is used. When free of protein, the dye is red but once bound to protein it turns blue. The dye-protein...

Word Count : 1069

Zymography

Last Update:

zymogram is subsequently stained (commonly with Amido Black or Coomassie brilliant blue), and areas of digestion appear as clear bands against a darkly...

Word Count : 812

Immunoelectrophoresis

Last Update:

the wet agarose gel, but are stained with protein stains like Coomassie brilliant blue in the dried gel. In contrast to SDS-gel electrophoresis, the electrophoresis...

Word Count : 1737

Levinstein Ltd

Last Update:

plant in Ellesmere Port. The firm made the successful Blackley blue or Coomassie brilliant blue, a Manchester Brown, a Manchester Yellow dye and during WWI...

Word Count : 122

Ponceau S

Last Update:

Ponceau S in 30% trichloroacetic acid and 30% sulfosalicylic acid. Coomassie brilliant blue Western blot normalization "Ponceau S solution". Sigma-Aldrich...

Word Count : 267

List of dyes

Last Update:

red Cotton red Direct red 28 22120 diazo 573-58-0 Coomassie brilliant blue Brilliant blue R Acid blue 83 42660 triarylmethane 6104-59-2 Cotton scarlet...

Word Count : 67

PDF Search Engine © AllGlobal.net