Electroblotting is a method in molecular biology/biochemistry/immunogenetics to transfer proteins or nucleic acids onto a membrane by using PVDF or nitrocellulose, after gel electrophoresis.[2][3] The protein or nucleic acid can then be further analyzed using probes such as specific antibodies, ligands like lectins, or stains. This method can be used with all polyacrylamide and agarose gels. An alternative technique for transferring proteins from a gel is capillary blotting.
^Cite error: The named reference Dubey2014 was invoked but never defined (see the help page).
^David Sheehan (30 April 2013). Physical Biochemistry: Principles and Applications. John Wiley & Sons. pp. 206–. ISBN 978-1-118-68748-2.
^M. P. Bansal (1 January 2013). Molecular Biology and Biotechnology: basic experimental protocols. The Energy and Resources Institute (TERI). pp. 141–. ISBN 978-81-7993-379-4.
Electroblotting is a method in molecular biology/biochemistry/immunogenetics to transfer proteins or nucleic acids onto a membrane by using PVDF or nitrocellulose...
limitations. Gel electrophoresis is often performed in combination with electroblotting or immunoblotting to give additional information about a specific protein...
Capillary electrophoresis Dielectrophoresis Free-flow electrophoresis Electroblotting Gel electrophoresis Gel electrophoresis of nucleic acids Gel electrophoresis...
proteins in very complex mixtures, which also employed methods of electroblotting and staining. Then, bands would be extracted from the gel and sequenced...
to the surface for extraction and subsequent analysis. For example, electroblotting and preparative native PAGE are based upon the same principle. Using...
commonly used method for transferring the proteins is called electroblotting. Electroblotting uses an electric current to pull the negatively charged proteins...
amounts of polyclonal antibodies. Today gel electrophoresis followed by electroblotting is the preferred method for protein characterization because its ease...
electrophoresis. Characterization through ligand interaction may be performed by electroblotting or by affinity electrophoresis in agarose or by capillary electrophoresis...
electrophoresis Capillary electrophoresis DNA electrophoresis Eastern blotting Electroblotting Fast parallel proteolysis (FASTpp) History of electrophoresis Isoelectric...
standard diffusion to transfer the proteins from the gel to the filter, electroblotting is commonly used because it removes the denaturing agent SDS thereby...
Vandekerckhove J (July 1990). "Two-dimensional gel electrophoresis, protein electroblotting and microsequencing: a direct link between proteins and genes". Electrophoresis...
Vandekerckhove J (1990). "Two-dimensional gel electrophoresis, protein electroblotting and microsequencing: a direct link between proteins and genes". Electrophoresis...
Bulcke M, et al. (1990). "Two-dimensional gel electrophoresis, protein electroblotting and microsequencing: a direct link between proteins and genes". Electrophoresis...
543–547. The second which was given the name “Mirror Image in vivo electroblotting technique” is designed to detect virus particles or any foreign protein...
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