Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa).
Infobox references
Chemical compound
Ponceau S, Acid Red 112, or C.I. 27195 (systematic name: 3-hydroxy-4-(2-sulfo-4-[4-sulfophenylazo]phenylazo)-2,7-naphthalenedisulfonic acid sodium salt[1]) is a sodium salt of a diazo dye of a light red color, that may be used to prepare a stain for rapid reversible detection of protein bands on nitrocellulose or polyvinylidene fluoride (PVDF) membranes (western blotting), as well as on cellulose acetate membranes.[2] A Ponceau S stain is useful because it does not appear to have a deleterious effect on the sequencing of blotted polypeptides and is therefore one method of choice for locating polypeptides on western blots for blot-sequencing.[2] It is also easily reversed with water washes, facilitating subsequent immunological detection.[2] The stain can be completely removed from the protein bands by continued washing.[2] Common stain formulations include 0.1% (w/v) Ponceau S in 5% acetic acid or 2% (w/v) Ponceau S in 30% trichloroacetic acid and 30% sulfosalicylic acid.[3]
A nitrocellulose membrane stained with Ponceau S dye for protein detection during western blotting. The blue bands on the left are protein markers for various molecular weights.
^ abcdAl-Amoudi, M. S.; Salman, Mahmoud; Al-Majthoub, M. M.; Adam, Abdel Majid A.; Alshanbari, Naif A.; Refat, Moamen S. (9 October 2013). "Spectral studies to increase the efficiency and stability of laser dyes by charge-transfer reactions for using in solar cells: charge-transfer complexes of Ponceau S with p-chloranil, chloranilic and picric acids". Research on Chemical Intermediates. 41 (5): 3089–3108. doi:10.1007/s11164-013-1417-4. S2CID 96999885.
^"PonceauS solution". Sigma-Aldrich. Retrieved 2016-11-24. The following are two common stain formulations…
up ponceau in Wiktionary, the free dictionary. Ponceau (French for "poppy-colored") may refer to: Ponceau 2R (also called Xylidine ponceau or Ponceau G...
Ponceau 4R (known by more than 100 synonyms,: 460–461 including as C.I. 16255, cochineal red A, C.I. acid red 18, brilliant scarlet 3R, brilliant scarlet...
(typically nitrocellulose or PVDF). The membrane is often then stained with PonceauS in order to visualize the proteins on the blot and ensure a proper transfer...
Peter Stephen Du Ponceau (born Pierre-Étienne du Ponceau, June 3, 1760 – April 1, 1844) was a French-American linguist, philosopher, and jurist. After...
Julien Ponceau (born 28 November 2000) is a professional footballer who plays as a midfielder for Ligue 1 club Lorient. Born in Angola, Ponceau is a youth...
PonceauS staining decreases quickly over time, so documentation should be conducted rapidly. A linear range of up to 140 µg is reported for Ponceau S...
Ponceau 6R, or Crystal ponceau 6R, Crystal scarlet, Brilliant crystal scarlet 6R, Acid Red 44, or C.I. 16250, is a red azo dye. It is soluble in water...
voluntarily stop using six food additive colours, tartrazine, allura red, ponceau 4R, quinoline yellow WS, sunset yellow and carmoisine (dubbed the "Southampton...
Ponceau 2R, Xylidine ponceau, Ponceau G, Red R, Acid Red 26, Food Red 5, or C.I. 16150 is a red azo dye used in histology for staining. It is easily soluble...
Agency commissioned a study of six food dyes (tartrazine, Allura red, Ponceau 4R, Quinoline Yellow, sunset yellow, carmoisine (dubbed the "Southampton...
Crimson 4R C.I. Food Red 1 Food red 1 FD & C Red no. 4 Hexacol Ponceau SX Maple Ponceau SX Ponceau SX Purple 4R E125 Identifiers CAS Number 4548-53-2 (Z) N...
Ponceau 3R (C.I. 16155) is an azo dye that once was used as a red food colorant. It is one of a family of Ponceau (French for "poppy-colored") dyes. Sharma...
their verbs.[citation needed] The term was invented by Peter Stephen Du Ponceau, who considered polysynthesis, as characterized by sentence words and noun...
include: E104: Quinoline yellow E122: Carmoisine E124: Ponceau 4R E131: Patent blue V E142: Green S The three synthetic colors Orange B, Citrus Red No. 2...
University of the effect of a mixture of six food dyes (Tartrazine, Allura Red, Ponceau 4R, Quinoline Yellow WS, Sunset Yellow and Carmoisine (dubbed the "Southampton...
1820. Former President Thomas Jefferson’s handwritten letter to Peter S. Du Ponceau, on July 7, 1820, states that a Nottoway Indian vocabulary was obtained...
assess the effect of a mixture of six food dyes (Tartrazine, Allura Red, Ponceau 4R, Quinoline Yellow WS, Sunset Yellow FCF and Carmoisine (dubbed the "Southampton...
(9%), tartrazine (11%), sunset yellow FCF (17%), allura red AC (16%), ponceau 4R (15%), erythrosine (12%) and brilliant blue FCF (14%). Annatto is not...
trichrome staining are: Red Acid fuchsin, xylidine ponceau, chromotrope 2R, Biebrich scarlet, ponceau 6R, phloxine Blue and green Light green SF yellowish...
industries, such as: Methyl orange Direct brown 138 Sunset yellow FCF Ponceau Most drugs and drug candidates contain amine functional groups: Chlorpheniramine...
Pontière, his military secretary, Peter Stephen Du Ponceau (then called Pierre Etienne Du Ponceau), and two other companions, reached Portsmouth, New...
kallas Pensylvania. Stockholm: J.H. Werner for Sal. Wankijfs. Peter S. Du Ponceau. 1834. "A Short Description of the Province of New Sweden, Now Called...
caffeine, antioxidant (ascorbic acid), colour (sunset yellow (E110), Ponceau 4R). After the reformulation to lower sugar in 2017, and another reformulation...
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