Biotechnological modification process to attach Biotin to a molecule
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In biochemistry, biotinylation is the process of covalently attaching biotin to a protein, nucleic acid or other molecule. Biotinylation is rapid, specific and is unlikely to disturb the natural function of the molecule due to the small size of biotin (MW = 244.31 g/mol). Biotin binds to streptavidin and avidin with an extremely high affinity, fast on-rate, and high specificity, and these interactions are exploited in many areas of biotechnology to isolate biotinylated molecules of interest. Biotin-binding to streptavidin and avidin is resistant to extremes of heat, pH and proteolysis, making capture of biotinylated molecules possible in a wide variety of environments. Also, multiple biotin molecules can be conjugated to a protein of interest, which allows binding of multiple streptavidin, avidin or neutravidin protein molecules and increases the sensitivity of detection of the protein of interest. There is a large number of biotinylation reagents available that exploit the wide range of possible labelling methods. Due to the strong affinity between biotin and streptavidin, the purification of biotinylated proteins has been a widely used approach to identify protein-protein interactions and post-translational events such as ubiquitylation[1] in molecular biology.
^Lectez, Benoît; Migotti, Rebekka; Lee, So Young; Ramirez, Juanma; Beraza, Naiara; Mansfield, Bill; Sutherland, James D.; Martinez-Chantar, Maria L.; Dittmar, Gunnar (2014-06-06). "Ubiquitin Profiling in Liver Using a Transgenic Mouse with Biotinylated Ubiquitin". Journal of Proteome Research. 13 (6): 3016–3026. doi:10.1021/pr5001913. ISSN 1535-3893. PMID 24730562.
biochemistry, biotinylation is the process of covalently attaching biotin to a protein, nucleic acid or other molecule. Biotinylation is rapid, specific...
acids and fatty acids, synthesis of fatty acids, and gluconeogenesis. Biotinylation of histone proteins in nuclear chromatin plays a role in chromatin stability...
assays. Luciferase can act as an ATP sensor protein through biotinylation. Biotinylation will immobilize luciferase on the cell-surface by binding to...
not). Protein tags may allow specific enzymatic modification (such as biotinylation by biotin ligase) or chemical modification (such as coupling to other...
rigorous optimisation of tissue fixation and processing methods. Protein biotinylation in vivo was proposed to alleviate the problems caused by frequent incompatibility...
ISBN 978-1-118-91840-1. Chapman-Smith A, Cronan JE (September 1999). "The enzymatic biotinylation of proteins: a post-translational modification of exceptional specificity"...
K. An epitope-tagged version of the protein of interest, or in vivo biotinylation can be used instead of antibodies to the native protein of interest...
(August 2015). "A Cell-Permeable ATP Analogue for Kinase-Catalyzed Biotinylation". Angewandte Chemie. 54 (33): 9618–9621. doi:10.1002/anie.201503041...
used will likely occur by treatment with a phosphorylation enzyme and biotinylation and reaction of the biotinylated substance with the dye. It is possible...
that the antibody can bind to the new streptavidin-enzyme conjugate. Biotinylation basically creates a strong affinity between the biotin on the cytokine-specific...
nonspecific labeling of proteins, DNA and RNA probes or other molecules. Biotinylation of DNA and RNA with photoactivatable biotin is easier and less expensive...
30 October 2007. Chapman-Smith A, Cronan JE (1999). "The enzymatic biotinylation of proteins: a post-translational modification of exceptional specificity"...
has been to attach the exonuclease to the nanopore, perhaps through biotinylation to the beta barrel hemolysin. The central pore of the protein may be...
Citrulline residues can be chemically modified with butanedione or by biotinylation prior to analysis, leading to a different mass shift, and this strategy...
Michael R; Gruenbaum, Yosef; Collins, Francis S (February 2018). "Biotinylation by antibody recognition - A method for proximity labeling". Nature Methods...
accessible tumor antigens in human colon cancer by ex vivo protein biotinylation and comparative mass spectrometry analysis". Int. J. Cancer. 123 (12):...
readily isolated from cells attached to glass. The advent of proximity biotinylation by birA* has facilitated the first proteomics-based studies of the cadherin...
Biotinylated primers designed to bind to the tags are used to amplify the DNA. Biotinylation allows the amplified DNA to bind to the magnetic beads, which are coated...
PMID 11106165. Chapman-Smith A, Cronan JE (September 1999). "The enzymatic biotinylation of proteins: a post-translational modification of exceptional specificity"...
proteolysis products. Acetylation of amines followed by tryptic digestion and biotinylation of free N-terminal peptides uses chemical (acetylation) to label free...
antiparallel β-strands that wrap around a central hydrophobic core. The biotinylation motif Met-Lys-Met is located at the tip of the β-hairpin structure....
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