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Ouchterlony double immunodiffusion information


Picture of an Ouchterlony double immunodiffusion plate, after immunodiffusion has taken place. In this, titre value of an antigen is quantified. The central well has an antibody, and the surrounding wells have decreasing concentration of the corresponding antigen.
Ouchterlony patterns showing no identity between upper spots
Ouchterlony patterns showing full identity between upper spots
Ouchterlony patterns showing partial identity between upper spots

Ouchterlony double immunodiffusion (also known as passive double immunodiffusion) is an immunological technique used in the detection, identification and quantification of antibodies and antigens, such as immunoglobulins and extractable nuclear antigens. The technique is named after Örjan Ouchterlony, the Swedish physician who developed the test in 1948 to evaluate the production of diphtheria toxins from isolated bacteria.[1]

  1. ^ Hornbeck, Peter (February 2017). "Double-Immunodiffusion Assay for Detecting Specific Antibodies (Ouchterlony)". Current Protocols in Immunology. 116 (1): 2.3.1–2.3.4. doi:10.1002/cpim.18. PMID 28150863. S2CID 35908711.

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Ouchterlony double immunodiffusion

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Ouchterlony double immunodiffusion (also known as passive double immunodiffusion) is an immunological technique used in the detection, identification and...

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Immunodiffusion

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diffusion in two dimensions (Ouchterlony double immunodiffusion) Coleman, R. M.; Kaufman, L. (1972). "Use of the Immunodiffusion Test in the Serodiagnosis...

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Gel

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chromatography Hydrocolloid Ouchterlony double immunodiffusion Paste (rheology) Polyacrylamide gel electrophoresis Radial immunodiffusion Silicone gel Two-dimensional...

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Ochterlony

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a monument in Kolkata. India Ouchterlony double immunodiffusion, an immunological technique named after Örjan Ouchterlony This disambiguation page lists...

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Agar

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Phytophthora sojae Ouchterlony double immunodiffusion – Biomedical technique R2A agar – Bacterial culture medium Radial immunodiffusion – Laboratory technique...

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Ancient protein

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1980s, utilising different immunological techniques such as Ouchterlony double immunodiffusion (interactions of antibodies and antigens in a gel). Ostrom...

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Auchterlonie

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Mills Edition, 1987 "Ouchterlony Test". Enotes.com. Retrieved April 11, 2010. Ouchterlony O (1970), Handbook of Immunodiffusion and Immunoelectrophoresis...

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Outline of immunology

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Immunoprecipitation Chromatin immunoprecipitation Immunodiffusion Ouchterlony double immunodiffusion Radial immunodiffusion Immunoelectrophoresis Counterimmunoelectrophoresis...

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Extractable nuclear antigen

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detection are Ro, La, Sm, RNP, Scl-70 and Jo1, which are screened for by Ouchterlony double immuno diffusion techniques and confirmed by immunoblotting. On anti-nuclear...

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