Conditional gene knockout is a technique used to eliminate a specific gene in a certain tissue, such as the liver.[1][2] This technique is useful to study the role of individual genes in living organisms. It differs from traditional gene knockout because it targets specific genes at specific times rather than being deleted from beginning of life. Using the conditional gene knockout technique eliminates many of the side effects from traditional gene knockout. In traditional gene knockout, embryonic death from a gene mutation can occur, and this prevents scientists from studying the gene in adults. Some tissues cannot be studied properly in isolation, so the gene must be inactive in a certain tissue while remaining active in others. With this technology, scientists are able to knockout genes at a specific stage in development and study how the knockout of a gene in one tissue affects the same gene in other tissues.[3][4]
^Varshney, Guarav; Burgess, Shawn (26 October 2013). "Mutagenesis and phenotyping resources in zebrafish for studying development and human disease". Briefings in Functional Genomics. 13 (2): 82–94. doi:10.1093/bfgp/elt042. PMC 3954039. PMID 24162064.
^Skarnes, William; Rosen, Barry; et al. (2011). "A conditional knockout resource for the genome-wide study of mouse gene function". Nature. 474 (7351): 337–342. doi:10.1038/nature10163. PMC 3572410. PMID 21677750.
^Clarke, Alan (21 March 2000). "Manipulating the germline: its impact on the study of carcinogenesis". Carcinogenesis. 21 (3): 435–441. doi:10.1093/carcin/21.3.435. PMID 10688863.
^Zhang, Jian; Zhao, Jing (July 2012). "Conditional gene manipulation: Cre-ating a new biological era". J Zhejiang Univ Sci B. 13 (7): 511–524. doi:10.1631/jzus.b1200042. PMC 3390709. PMID 22761243.
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Conditionalgeneknockout is a technique used to eliminate a specific gene in a certain tissue, such as the liver. This technique is useful to study the...
complete and conditional. A complete geneknockout permanently inactivates the gene, while a conditionalgeneknockout allows for the gene to be turned...
Another limitation is that conventional (i.e. non-conditional) knockout mice develop in the absence of the gene being investigated. At times, loss of activity...
create knockout mice to study. Many of the targeted alleles are designed so that they can generate both complete and conditionalgeneknockout mice. The...
leading to the activation of different target genes. In situ hybridization and conditionalgeneknockout studies have helped to demonstrate the role JAG1...
A knockout rat is a genetically engineered rat with a single gene turned off through a targeted mutation (gene trapping) used for academic and pharmaceutical...
capable of disrupting genes in a manner that is specific to only heart tissue in vivo and allows for the creation of conditionalknockouts of the heart mostly...
mice. This conditionality is a key property of the entire resource, and it allows a more nuanced study of the effects of the gene-knockout. The purpose...
interference (RNAi) do not fully suppress gene function, CRISPR, ZFNs, and TALENs provide full irreversible geneknockout. CRISPR can also target several DNA...
study of 900 mouse genes concluded that 42% of them were essential although the selected genes were not representative. Geneknockout experiments are not...
create null alleles so that the gene is not functional. For example, deletion of a gene by gene targeting (geneknockout) can be done in some organisms...
the primary IMPC phenotyping pipeline and a conditional ready allele that allows tissue restricted knockouts via the Cre-Lox Recombination and FLP-FRT recombination...
Bushell W, Iyer V, et al. (Jun 2011). "A conditionalknockout resource for the genome-wide study of mouse gene function". Nature. 474 (7351): 337–42. doi:10...
shown to have the highest level of SBK3 expression. A novel conditional nebulin knockout mouse model revealed an increase in SBK3 expression in the quadriceps...
geneknockout and other assays, although frontiers of bioinformatics research are making it increasingly possible to predict the function of a gene based...
determined through the phenotype presented in conditional Sec14p mutants. As Sec14p is an essential gene, Sec14p knockouts must be performed in yeast strains with...
gestation. Thus, to study the role of Lim-1 on the mesonephros, a conditionalknockout model was employed in mice whereby Lim-1 was selectively removed...
encoded by the DMRT1 gene. DMRT1 is a dose sensitive transcription factor protein that regulates Sertoli cells and germ cells. The DMRT1 gene is located at the...
peptides need to be experimentally verified. Knockout mice of GPX4 die at embryonic day 8 and conditional inducible deletion in adult mice (neurons) results...
logic. Tests of necessity, among which are methods of lesioning or geneknockout, and tests of sufficiency, among which are methods of isolation or discrete...
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