Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa).
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Chemical compound
Safranin (Safranin O or basic red 2) is a biological stain used in histology and cytology. Safranin is used as a counterstain in some staining protocols, colouring cell nuclei red. This is the classic counterstain in both Gram stains and endospore staining. It can also be used for the detection of cartilage,[2] mucin and mast cell granules.
Safranin typically has the chemical structure shown at right (sometimes described as dimethyl safranin). There is also trimethyl safranin, which has an added methyl group in the ortho- position (see Arene substitution pattern) of the lower ring. Both compounds behave essentially identically in biological staining applications, and most manufacturers of safranin do not distinguish between the two. Commercial safranin preparations often contain a blend of both types.
Safranin is also used as redox indicator in analytical chemistry.
^ abcd"Safety Data Sheet: Safranin O" (PDF). LabChem. Retrieved 10 March 2016.
^Rosenberg L (1971). "Chemical Basis for the Histological Use of Safranin O in the Study of Articular Cartilage". J Bone Joint Surg Am. 53 (1): 69–82. doi:10.2106/00004623-197153010-00007. PMID 4250366. Archived from the original (abstract) on 2008-04-17.
Safranin (Safranin O or basic red 2) is a biological stain used in histology and cytology. Safranin is used as a counterstain in some staining protocols...
of ethanol. They are stained pink or red by the counterstain, commonly safranin or fuchsine. Lugol's iodine solution is always added after addition of...
alcohol removes the crystal violet colour from gram negative organisms only. Safranin as counterstain is used to colour the gram negative organisms that got...
when safranin is added during the process, they stain red. The Mycoplasmataceae lack a peptidoglycan layer so do not retain crystal violet or safranin, resulting...
endospores could not be stained using simple stains such as methylene blue, safranin, and carbol fuchsin. These scientists, along with a few others, found out...
During the staining process, E. coli picks up the color of the counterstain safranin and stains pink. The outer membrane surrounding the cell wall provides...
Gram stain. Gram staining uses two dyes: Crystal violet and Fuchsin or Safranin (the counterstain) to differentiate between Gram-positive bacteria (large...
Gram staining, crystal violet stains only Gram-positive bacteria, and safranin counterstain is applied which stains all cells, allowing the identification...
selectively stain cells. Other compounds used to color tissue sections include safranin, Oil Red O, congo red, silver salts and artificial dyes. Histochemistry...
since it can directly stain endospores within bacterial cells; here a safranin counterstain is often used. Malachite green is a part of Alexander's pollen...
Bacillus megaterium Bacillus megaterium cells stained with Sudan Black B and safranin. Scientific classification Domain: Bacteria Phylum: Bacillota Class: Bacilli...
is made using a primary stain of crystal violet and a counterstain of safranin. Bacteria that turn purple when stained are termed 'Gram-positive', while...
is required. The bacteria are stained with the dyes crystal violet and safranin. Gram positive cells are purple after staining, while Gram negative cells...
rinse in distilled water and application of a counterstain such as eosin, safranin or neutral red. Potassium ferrocyanide in the staining solution combines...
effectively visualise the organism's shape, a staining technique involving safranin is recommended, followed by observation under oil immersion. Furthermore...
acid +0.26 -0.16 blue colorless Phenosafranin +0.28 -0.25 red colorless Safranin T +0.24 -0.29 red-violet colorless Neutral red +0.24 -0.33 red colorless...
became a commercial dye. Other synthetic dyes followed, such as fuchsin, safranin, and induline. At the time of mauveine's discovery, aniline was expensive...
the Crystal Violet - Iodine complex, but will be counter stained red by Safranin. The optimal growth temperature on a Nutrient Agar plate is 30-35 degree...
acid-Schiff (PAS) can be performed, as the walls stain positively. Heated safranin + methylene blue has been used for staining, as well. PCR amplification...
reactant, oxygen, is consumed by another reactant, benzoin, with the help of safranin as a catalyst. Once the limited amount of oxygen has been used up, the...
paraffin hydrocarbons, the compounds aurin, rosaniline, suberone, and safranin, and topics in the history of chemistry. Towards the end of Schorlemmer's...
independent scientific work was to determine the structure of the dyestuff safranin, for the German chemical company Cassella. Around that time, he also discovered...
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