Transposon mutagenesis, or transposition mutagenesis, is a biological process that allows genes to be transferred to a host organism's chromosome, interrupting or modifying the function of an extant gene on the chromosome and causing mutation.[1] Transposon mutagenesis is much more effective than chemical mutagenesis, with a higher mutation frequency and a lower chance of killing the organism. Other advantages include being able to induce single hit mutations, being able to incorporate selectable markers in strain construction, and being able to recover genes after mutagenesis.[2] Disadvantages include the low frequency of transposition in living systems, and the inaccuracy of most transposition systems.
^Seifert, H S; Chen, E Y; So, M; Heffron, F (1986-02-01). "Shuttle mutagenesis: a method of transposon mutagenesis for Saccharomyces cerevisiae". Proceedings of the National Academy of Sciences of the United States of America. 83 (3): 735–739. Bibcode:1986PNAS...83..735S. doi:10.1073/pnas.83.3.735. ISSN 0027-8424. PMC 322939. PMID 3003748.
and 26 Related for: Transposon mutagenesis information
Transposonmutagenesis, or transposition mutagenesis, is a biological process that allows genes to be transferred to a host organism's chromosome, interrupting...
Rosen E, Fraser MJ (September 1989). "Transposonmutagenesis of baculoviruses: analysis of Trichoplusia ni transposon IFP2 insertions within the FP-locus...
into bacteria for such purposes as transformation, cloning, or transposonmutagenesis. Triparental matings can help overcome some of the barriers to efficient...
The Sleeping Beauty transposon system is a synthetic DNA transposon designed to introduce precisely defined DNA sequences into the chromosomes of vertebrate...
Currently transposons can be used in genetic research and recombinant genetic engineering for insertional mutagenesis. Insertional mutagenesis is when transposons...
site-directed mutagenesis to create point mutations and measure relative fitness of each mutant. In Escherichia coli, one study used transposonmutagenesis to directly...
transposase is any of a class of enzymes capable of binding to the end of a transposon and catalysing its movement to another part of a genome, typically by...
A transposable element (TE, transposon, or jumping gene) is a nucleic acid sequence in DNA that can change its position within a genome, sometimes creating...
completely deleted from the genome in a systematic way. In transposon-mediated mutagenesis, transposons are randomly inserted in as many positions in a genome...
pollinator specificity and attraction. Researchers carried out transposonmutagenesis in Antirrhinum majus, and saw that some insertions led to formation...
to find all the essential genes of M. genitalium through global transposonmutagenesis. As a result, they found that 382 out of 482 protein coding genes...
Alternatively, ENU mutagenesis is a common random mutagenesis gene knockout strategy in the mouse that can also be used in the rat. ENU mutagenesis involves using...
and Gene Trap TransposonMutagenesis in Arabidopsis, comprehensive article on the use of Ac/Ds and other transposons for plant mutagenesis Barbara McClintock...
most of its genes in viral replication was characterized by random transposonmutagenesis. Alpha-, beta-, and gammaherpesviruses display a heterodimer composed...
E S (1 September 1990). "Floral homeotic mutations produced by transposon-mutagenesis in Antirrhinum majus". Genes & Development. 4 (9): 1483–1493. doi:10...
Type VI secretion system and putative cognate effectors. Initial transposonmutagenesis screens revealed 195 essential genes, although this number is likely...
natural organism that can be grown in free culture). They used transposonmutagenesis to identify genes that were not essential for the growth of the...
intracellular actinomycete Rhodococcus equi created by in vivo Himar1 transposonmutagenesis". Journal of Bacteriology. 185 (8): 2644–52. doi:10.1128/jb.185...
in Xanthobacter autotrophicus Py2 using a new, highly efficient transposonmutagenesis system that is functional in a wide variety of bacteria". Archives...
can also be generated by insertional mutagenesis. Most often, insertional mutagenesis involves the use of transposons, which introduces dramatic changes...
Host Range Mobilization System for In Vivo Genetic Engineering: TransposonMutagenesis in Gram Negative Bacteria". Nature Biotechnology. 1 (9): 784–791...
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