Global InformationTriparental mating information
Triparental mating is a form of bacterial conjugation where a conjugative plasmid present in one bacterial strain assists the transfer of a mobilizable plasmid present in a second bacterial strain into a third bacterial strain.[1] Plasmids are introduced into bacteria for such purposes as transformation, cloning, or transposon mutagenesis. Triparental matings can help overcome some of the barriers to efficient plasmid mobilization. For instance, if the conjugative plasmid and the mobilizable plasmid are members of the same incompatibility group they do not need to stably coexist in the second bacterial strain for the mobilizable plasmid to be transferred.
- ^ Wise, Arlene A.; Liu, Zhenying; Binns, Andrew N. (2006), Wang, Kan (ed.), "Three Methods for the Introduction of Foreign DNA into Agrobacterium", Agrobacterium Protocols, vol. 343, Totowa, NJ: Humana Press, pp. 43–54, doi:10.1385/1-59745-130-4:43, ISBN 978-1-59745-130-7, PMID 16988332, retrieved 2022-06-13