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Transient expression, more frequently referred to "transient gene expression", is the temporary expression of genes that are expressed for a short time after nucleic acid, most frequently plasmid DNA encoding an expression cassette, has been introduced into eukaryotic cells with a chemical delivery agent like calcium phosphate (CaPi) or polyethyleneimine (PEI).[1] However, unlike "stable expression," the foreign DNA does not fuse with the host cell DNA, resulting in the inevitable loss of the vector after several cell replication cycles.[2] The majority of transient gene expressions are done with cultivated animal cells. The technique is also used in plant cells; however, the transfer of nucleic acids into these cells requires different methods than those with animal cells. In both plants and animals, transient expression should result in a time-limited use of transferred nucleic acids, since any long-term expression would be called "stable expression."
Methodology varies depending on the organism to transform. While plants can be transformed with a construct introduced into Agrobacterium tumefaciens via agroinfiltration or floral dip, most animal cells would require a viral vector. In humans, the field of transient transformation advanced rapidly during the 2020–2021 COVID-19 pandemic with major COVID-19 vaccines using either direct mRNA transfer into human or adenovirus vectors, with the RNA being expressed in the host human to produce spike proteins that induce an immune response.
^Hacker DL (2011-01-01). "2.29 - Recombinant Technology". In Moo-Young M, Wurm FM (eds.). Comprehensive Biotechnology (Second ed.). Burlington: Academic Press. pp. 401–406. doi:10.1016/b978-0-08-088504-9.00120-3. ISBN 978-0-08-088504-9.
^Glick BR, Patten CL (January 2017). Molecular Biotechnology: Principles and Applications of Recombinant DNA (Fifth ed.). doi:10.1128/9781555819378. ISBN 9781555819378.
and 26 Related for: Transient expression information
Transientexpression, more frequently referred to "transient gene expression", is the temporary expression of genes that are expressed for a short time...
the host DNA, causing permanent expression, or not integrated, causing transientexpression. Heterologous expression can be done in many types of host...
plant biology and especially lately in plant biotechnology to induce transientexpression of genes in a plant, or isolated leaves from a plant, or even in...
transformant, transientexpression allows short-term experiments to be done using labeled or modified genes in C. merolae. Transientexpression can be achieved...
engine Sharpe Field (IATA: TGE), a private American airport Transientexpression, a gene expression Transitional Government of Ethiopia This disambiguation...
only cells in immediate contact with the bacteria, and results in transientexpression of plasmid DNA. Agroinfiltration is commonly used to transform tobacco...
Gene expression is the process by which information from a gene is used in the synthesis of a functional gene product that enables it to produce end products...
delivered to the cells, the DNA is used as a template for transcription (transientexpression) and sometimes it integrates into a plant chromosome ('stable' transformation)...
Characteristics". Retrieved 28 November 2017. Aruffo, A (November 2002). "Transientexpression of proteins using COS cells". Curr Protoc Mol Biol. Chapter 16: Unit...
disadvantage depending on whether the application demands persistent or transientexpression). A novel addition to the field are nonviral self-replicating minicircles...
L, Candusso ME, Leiter AB, Rindi G, Merighi A (December 2004). "Transientexpression of secretin in serotoninergic neurons of mouse brain during development"...
Transient receptor potential channels (TRP channels) are a group of ion channels located mostly on the plasma membrane of numerous animal cell types....
PMID 3560174. Orkin SH, Goff SC, Kelley WN, Daddona PE (Apr 1985). "Transientexpression of human adenosine deaminase cDNAs: identification of a nonfunctional...
differences compared to commonly known transientexpression and stable cell line expression systems. Unlike in transientexpression system, QMCF Technology enables...
(July 2013). "Efficient agroinfiltration of plants for high-level transientexpression of recombinant proteins". Journal of Visualized Experiments. 77 (77)...
immuno-contraception, transmissible vaccines, and agricultural field transientexpression systems are not examples of HEGAA approaches, because none currently...
Miller AL (2006). "Transientexpression of apoaequorin in zebrafish embryos: extending the ability to image calcium transients during later stages of...
inhibitor of rice (oryzacystatin). Homology with animal cystatins and transientexpression in the ripening process of rice seeds". The Journal of Biological...
also classified as motor disorders. ICD10 diagnosis codes are: F95.0 Transient tic disorder F95.1 Chronic motor or vocal tic disorder F95.2 Combined...
Gaubert, S.; Leroux-Coyau, M.; Prince, S.; Houdebine, LM. (2004). "Transientexpression in mammalian cells of transgenes transcribed from the Cauliflower...
The transient receptor potential cation channel subfamily V member 1 (TRPV1), also known as the capsaicin receptor and the vanilloid receptor 1, is a...
cardiac myocytes results in apical stunning and transient cardiomyopathy. Microvascular dysfunction/Transient vasospasm: Some of the original researchers...
occurs and it results in the expression of a genetically modified gene this will often be referred to as a transientexpression approach. The capacity to...
P (Apr 2000). "Heparan N-sulfatase gene: two novel mutations and transientexpression of 15 defects". Biochimica et Biophysica Acta (BBA) - Molecular Basis...
interference refers to a family of gene silencing effects by which gene expression is negatively regulated by non-coding RNAs such as microRNAs. RNA silencing...
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