Introduction of foreign genetic material into host cells
Gene delivery is the process of introducing foreign genetic material, such as DNA or RNA, into host cells.[1] Gene delivery must reach the genome of the host cell to induce gene expression.[2] Successful gene delivery requires the foreign gene delivery to remain stable within the host cell and can either integrate into the genome or replicate independently of it.[3] This requires foreign DNA to be synthesized as part of a vector, which is designed to enter the desired host cell and deliver the transgene to that cell's genome.[4] Vectors utilized as the method for gene delivery can be divided into two categories, recombinant viruses and synthetic vectors (viral and non-viral).[2][5]
In complex multicellular eukaryotes (more specifically Weissmanists), if the transgene is incorporated into the host's germline cells, the resulting host cell can pass the transgene to its progeny. If the transgene is incorporated into somatic cells, the transgene will stay with the somatic cell line, and thus its host organism.[6]
Gene delivery is a necessary step in gene therapy for the introduction or silencing of a gene to promote a therapeutic outcome in patients and also has applications in the genetic modification of crops. There are many different methods of gene delivery for various types of cells and tissues.[6]
^Jones CH, Chen CK, Ravikrishnan A, Rane S, Pfeifer BA (November 2013). "Overcoming nonviral gene delivery barriers: perspective and future". Molecular Pharmaceutics. 10 (11): 4082–98. doi:10.1021/mp400467x. PMC 5232591. PMID 24093932.
^ abKamimura K, Suda T, Zhang G, Liu D (October 2011). "Advances in Gene Delivery Systems". Pharmaceutical Medicine. 25 (5): 293–306. doi:10.1007/bf03256872. PMC 3245684. PMID 22200988.
^Mali S (January 2013). "Delivery systems for gene therapy". Indian Journal of Human Genetics. 19 (1): 3–8. doi:10.4103/0971-6866.112870. PMC 3722627. PMID 23901186.
^Gibson G, Muse SV (2009). A Primer of Genome Science (Third ed.). 23 Plumtree Rd, Sunderland, MA 01375: Sinauer Associates. pp. 304–305. ISBN 978-0-87893-236-8.{{cite book}}: CS1 maint: location (link)
^Pack DW, Hoffman AS, Pun S, Stayton PS (July 2005). "Design and development of polymers for gene delivery". Nature Reviews. Drug Discovery. 4 (7): 581–93. doi:10.1038/nrd1775. PMID 16052241. S2CID 20972049.
^ abNayerossadat N, Maedeh T, Ali PA (6 July 2012). "Viral and nonviral delivery systems for gene delivery". Advanced Biomedical Research. 1: 27. doi:10.4103/2277-9175.98152. PMC 3507026. PMID 23210086.
Genedelivery is the process of introducing foreign genetic material, such as DNA or RNA, into host cells. Genedelivery must reach the genome of the host...
Look up delivery or deliver in Wiktionary, the free dictionary. Delivery may refer to: Childbirth Drug deliveryGenedeliveryDelivery (commerce), of goods...
In genetic engineering, a gene gun or biolistic particle delivery system is a device used to deliver exogenous DNA (transgenes), RNA, or protein to cells...
January 2021). "Delivery of CRISPR/Cas systems for cancer gene therapy and immunotherapy". Advanced Drug Delivery Reviews. Delivery of Biomacromolecules...
A tumor suppressor gene (TSG), or anti-oncogene, is a gene that regulates a cell during cell division and replication. If the cell grows uncontrollably...
nondividing cells, so they are one of the most efficient methods of genedelivery. They can become endogenous, integrating their genome into the host...
gene therapy has gained significant attention from the medical community, especially for cancer therapy, a lack of safe and efficient genedelivery vectors...
Gene therapy utilizes the delivery of DNA into cells, which can be accomplished by several methods, summarized below. The two major classes of methods...
(viruses) that are used as carriers. There are many different methods of genedelivery developed for various types of cells and tissues, from bacterial to...
investigate the application of SBF in genedelivery. Calcium phosphate nanoparticles, required for the delivery of plasmid DNA (pDNA) into the nucleus...
efficient genedelivery vehicle. Likewise, the HIV-1 Tat peptide, a cell-permeable peptide, has been successfully used for intracellular genedelivery. Tetraethylenepentamine...
This article contains a list of commercially available gene therapies. Alipogene tiparvovec (Glybera): AAV-based treatment for lipoprotein lipase deficiency...
vectors have been developed to carry therapeutic genes to cells. There are two broad categories of genedelivery vectors: Viral vectors, involving viruses as...
Nayerossadat N, Maedeh T, Ali PA (6 July 2012). "Viral and nonviral delivery systems for genedelivery". Advanced Biomedical Research. 1: 27. doi:10.4103/2277-9175...
Lentiviral vectors in gene therapy is a method by which genes can be inserted, modified, or deleted in organisms using lentiviruses. Lentiviruses are...
bulk electroporation has many benefits over physical delivery methods such as microinjections and gene guns, it still has limitations, including low cell...
(June 2008). "Microbubbles in ultrasound-triggered drug and genedelivery". Advanced Drug Delivery Reviews. 60 (10): 1153–1166. doi:10.1016/j.addr.2008.03...
Hydrodynamic Delivery (HD) is a method of DNA insertion in rodent models. Genes are delivered via injection into the bloodstream of the animal, and are...
is produced. Generally speaking, expression of a foreign gene requires restructuring the gene to include sequences that are required for producing an mRNA...
drastic structural change can be used for programmed drug or genedelivery, in which the drug or gene can be released with the rings when the stoppers are cut...
and suicide genedelivery. Gene overexpression adds genetic sequences that compensate for low to zero levels of needed gene expression. Gene knockout uses...
which put the therapeutic genes under the control of the cancer cell specific promoters. The delivery of the suicidal genes to the cancer cells involves...
"Adenovirus-Mediated GeneDelivery: Potential Applications for Gene and Cell-Based Therapies in the New Era of Personalized Medicine". Genes & Diseases. 4 (2):...
tools in molecular biology, and they have been used successfully in genedelivery systems. Evidence from endogenous retroviruses (inherited provirus DNA...
Cationic liposomes are increasingly being researched for use as delivery vectors in gene therapy due to their capability to efficiently transfect cells...
tumor necrosis factor-related apoptosis-induced ligand) for effective genedelivery and tracking, with positive results. This is a TRAIL ligand that has...
An environment's plasmidome refers to the plasmids present in it. The term is a portmanteau of the two English words Plasmid and Kingdom. In biological...
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