Aequorin ribbon diagram from PDB 1ej3 with prosthetic group coelenterazine in blue
Identifiers
Organism
Aequorea victoria (Jellyfish)
Symbol
N/A
UniProt
P07164
Other data
EC number
1.13.12.5
Search for
Structures
Swiss-model
Domains
InterPro
Aequorin is a calcium-activated photoprotein isolated from the hydrozoan Aequorea victoria.[1] Its bioluminescence was studied decades before the protein was isolated from the animal by Osamu Shimomura in 1962.[2] In the animal, the protein occurs together with the green fluorescent protein to produce green light by resonant energy transfer, while aequorin by itself generates blue light.
Discussions of "jellyfish DNA" that can make "glowing" animals often refer to transgenic animals that express the green fluorescent protein, not aequorin, although both originally derive from the same animal.
Apoaequorin, the protein portion of aequorin, is an ingredient in the dietary supplement Prevagen. The US Federal Trade Commission (FTC) has charged the maker with false advertising for its memory improvement claims.
^Shimomura O (1995). "A short story of aequorin". Biol. Bull. 189 (1). Biological Bulletin: 1–5. doi:10.2307/1542194. JSTOR 1542194. PMID 7654844.
^Shimomura O, Johnson FH, Saiga Y (1962). "Extraction, purification and properties of aequorin, a bioluminescent protein from the luminous hydromedusan, Aequorea". J Cell Comp Physiol. 59 (3): 223–39. doi:10.1002/jcp.1030590302. PMID 13911999.
Aequorin is a calcium-activated photoprotein isolated from the hydrozoan Aequorea victoria. Its bioluminescence was studied decades before the protein...
west coast of North America. The species is best known as the source of aequorin (a photoprotein), and green fluorescent protein (GFP); two proteins involved...
protein aequorin from thousands of jellyfish until they gathered enough for a full analysis of the protein. It was through the study of aequorin that Shimomura...
cofactors may be required, such as calcium (Ca2+) for the photoprotein aequorin, or magnesium (Mg2+) ions and ATP for the firefly luciferase. Generically...
the 1960s and 1970s, GFP, along with the separate luminescent protein aequorin (an enzyme that catalyzes the breakdown of luciferin, releasing light)...
proportional to the amount of the photoprotein. For example, the photoprotein aequorin produces a flash of light when luciferin and calcium are added, rather...
occurs when a secondary substrate is added to the bioassay (luxAB, Luc, and aequorin). For other bioreporters, the signal must be activated by an external light...
known for his work to clone and sequence the genes for the photoprotein aequorin and green fluorescent protein (GFP) and for his proposal to use GFP as...
domain – InterPro: IPR000261 Aequorin is a calcium binding protein (CaBP) isolated from the cnidarian Aequorea victoria. Aequorin belongs to the EF-hand family...
chaetognaths, fish, and shrimp. It is the prosthetic group in the protein aequorin responsible for the blue light emission. Dinoflagellate luciferin is a...
green fluorescent protein (GFP) and another bioluminescent protein, called aequorin, from the large and abundant hydromedusa Aequorea victoria, while studying...
scientists to test drugs in the pre-clinical stages cheaply. Another protein, aequorin, found in certain jellyfish, produces blue light in the presence of calcium...
Prendergast FG, Mann KG (August 1978). "Chemical and physical properties of aequorin and the green fluorescent protein isolated from Aequorea forskålea". Biochemistry...
victoria, and consists of a 27-kDa green fluorescent protein and the aequorin complex which consists of a 22-kDa apoaequorin, the luminophore coelenterazine...
luciferase (Rluc), Gaussia luciferase (Gluc), and photoproteins, including aequorin, and obelin. All these proteins catalyze the oxidation of this substance...
neighboring radial canals. Despite containing a bioluminescent protein, aequorin, this species (as well as all other species in the genus) are almost completely...
Washington. In 1962, their work culminated in the discovery of the proteins aequorin and green fluorescent protein (GFP) in A. victoria; for this work, he was...
short-term labeling of cells. Alternatively, Fura-2 , Furaptra , Indo-1 and aequorin may be used. An acetomethoxy group obscures the part of the molecule that...
in addition to higher signal-to-noise ratios. Such systems may rely on aequorin and the luciferin coelenterazine. Ca2+ binding causes a conformational...
fluorescent proteins (GFP), along with the separate luminescent protein aequorin (an enzyme that catalyzes the breakdown of luciferin, releasing light)...
University of Malaga (Emilio Navarro). It is based on yeast cells expressing aequorin protein sensitive to change in intracellular calcium. Upon electrical stimulation...
salina) and rotifers (Brachionus plicatilis). It is bioluminescent due to aequorin and green fluorescent protein. "WoRMS - World Register of Marine Species...
Prendergast FG, Mann KG (1978-08-22). "Chemical and physical properties of aequorin and the green fluorescent protein isolated from Aequorea forskalea". Biochemistry...
Global Information